Levels of interleukin-6, superoxide dismutase and malondialdehyde in the lung tissue of a rat model of hypoxia-induced acute pulmonary edema

Abstract

Abstract. The present study aimed to investigate the levels of malondialdehyde (MDA), superoxide dismutase (SOD) and interleukin (IL)-6 in the lung tissue of a rat model of acute pulmonary edema induced by acute hypoxia, and its pathophysiological significance. A total of 48 adult Wistar rats were randomly divided into group A, a normal group; group B, a model of acute pulmonary edema induced by hypoxia for 24 h; group C, a model of acute pulmonary edema induced by hypoxia for 48 h; and group D, a model of acute pulmonary edema induced by hypoxia for 72 h. The rats in groups B-D were intraperitoneally injected with 6% ammonium chloride to establish the model of acute pulmonary edema, and were subsequently sacrificed following successful modeling for 24, 48 and 72 h. The plasma of rats was isolated and the lungs of the rats were removed. Subsequently, a 10% lung homogenate was prepared and the contents and the activities of MDA, SOD and IL-6 in the lung tissue and IL-6 in the plasma were detected by enzyme-linked immunosorbent assay. MDA and IL-6 expression levels increased and SOD activity decreased in the lung tissue in group B as compared with group A; however the difference did not reach significance (P>0.05). MDA, IL-6 and SOD levels in the lung tissue of rats were significantly altered following the increased duration of pulmonary edema in groups C and D, as compared group A (P<0.05). The plasma IL-6 levels of the rats in groups B-D significantly increased, as compared with those in group A (P<0.05). In conclusion, the results of the present study demonstrated that the incidence of acute pulmonary edema may be associated with oxidative stress. Furthermore, decreased antioxidant capacity and increased free radical levels may be associated with pulmonary edema, as in the present study the levels of IL-6, SOD and MDA in the lung tissue were observed to be associated with the pathological changes of the disease.

Angiotensin-(1–7) inhibits inflammation and oxidative stress to relieve lung injury induced by chronic intermittent hypoxia in rats

“…IL-8 functions include chemotaxis and activation of neutrophils, as well as chemotaxis of basophils, T lymphocytes, and other inflammatory cells ( 4 ). Decreased antioxidant capacity and increased free radical levels have been implicated in the pathological changes associated with pulmonary diseases ( 5 ). Superoxide dismutase (SOD) is a critical antioxidant that can effectively eliminate superoxide anions and protect cells from oxidative damage.…”

“…Proof of the efficacy of angiotensin-( 1 – 7 ) [Ang-(1–7)] as a novel therapeutic agent for many diseases has been revealed in preclinical studies. Angiotensin-converting enzyme 2 (ACE-2) degrades Ang II by removal of a single amino acid to generate the heptapeptide Ang-(1–7).…”

Sinomenine Attenuates Chronic Intermittent Hypoxia-Induced Lung Injury by Inhibiting Inflammation and Oxidative Stress

“…Antioxidant capacity reduction and free radical levels elevation have been reported to be involved in the pathological changes of pulmonary diseases [ 30 ]. The effect of SIN on oxidative stress was determined by detecting MDA content, SOD activity, and CAT level in lung tissues.…”

Low-Dose Pulsatile Interleukin-6 As a Treatment Option for Diabetic Peripheral Neuropathy

“…All three studies reported that IL-6 treatment resulted in improved or normalized nerve function and/or morphology. While none of these studies reported the corresponding plasma levels of IL-6 following administration, there are literature reports to establish normal rat plasma IL-6 levels to be 50–100 pg/mL ( 62 – 64 ), and after a single intraperitoneal injection of 25 µg/kg plasma IL-6 levels peaked at ~5,500 pg/mL dropping off to near baseline within 4 h ( 65 ). Taken together, the data demonstrate a transient 50- to 100-fold increase in IL-6 following a single dose administration may represent therapeutic range.…”