Levels of lactate dehydrogenase in osteoarthritic and failed total knee joints

Messieh, Michael

doi:10.1016/s0883-5403(96)80094-5

Cited by 5 publications

(3 citation statements)

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“…In osteoarthritis the most affected tissue is the articular cartilage and may be the most probable source of isoenzyme leakage. Therefore, it is likely that, similar to other pathological states of sLDH elevation (23,25,(40)(41), the higher levels of LDH noted in our case are related to the state of inflammation in OA. Our results may therefore provide evidence to prefer serum over the con ventional source, synovial fluid, for protein profiling and enzyme activity determination, and further recommend its use for the search of suitable molecular markers in case of osteoarthritis.…”

Section: Discussionsupporting

confidence: 73%

“…It may, therefore, appear that the expression of these isoforms is related to the pathophysiology of the disease and this needs to be evaluated further in a relatively larger sample size of the OA subjects. Evidence also suggest that lactate dehydrogenase has been very widely used as a marker of rheumatoid and osteoarthritis wherein an increase in LDH-4 and -5 and decrease in LDH-1 is reported (23,25,(40)(41). During the present investigation, we also observed a significant increase of 5% in the activity of LDH-5 (P<0.05) and a decrease of 7 and 2% in the activities of LDH-1 and -2 respectively in OA subjects compared with control (P<0.05).…”

Section: Discussionmentioning

confidence: 99%

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Analysis of Serum Proteins and Enzymes Level in Human Subjects with Osteoarthritis

Ahmad¹,

Irshad²,

Ahmad³

et al. 2011

Journal of Medical Biochemistry

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Kratak sadr`aj: Cilj ove studije bio je da se pomo}u profila PAGE odrede nivoi proteina i enzima u serumu subjekata sa osteoartritisom (OA). Za studiju je izabrana 41 osoba sa potvr|enom dijagnozom OA. Uzorci seruma su u jednakim koli~inama zasebno stavljeni na PAGE sa i bez denaturacije. Softverska analiza ovih profila obavljena je pomo}u pro grama Scion Imaging (Beta release-4, Scion Corporation) and GelPro (Media Cybernetics, SAD). Radi vizualizacije esteraza i (Est) izoenzima laktat dehidrogenaze (LDH) u serumu ovih pacijenata izvr{eno je bojenje specifi~nim supstratom. Razlike u vrednostima kontrolnih i osoba sa OA su statisti~ki testirane. Softverska analiza PAGE profila bez denaturacije otkrila je prisustvo devetnaest peptida u kontroli i dvadeset jednog u osobama sa OA. Dva dodatna peptida bila su prisutna u regionu gama-globulina kod osoba sa OA. Zna~ajan pad sa 42,77% na 36,72 % nivoa albumina (hipoalbuminemia) prime}en je kod osoba sa OA a odnos ukupnog albumina i globulina bio je 0,58. U SAD-PAGE, razlika izme|u kontrola i osoba sa OA uo~ena je izme|u osam peptida molekularne te`ine 25, 22 i 20 kDa (kojih nema u OA) i pet novih peptida od 270, 125, 30, 21,36 i 18,5 kDa (kojih nema kod kontrola), dok je albumin zadr`ao najve}u aktivnost. Za enzime, Est prate relativni poredak BchEst (42,86%) > ArylEst (16,24%) > AchEst (6,85%) kod osoba sa OA uz ekspresiju nove izoforme BchEst sa 4,78% i dve izoforme ArylEst u koncentracijama od 2,13 i 1,61%. Esterazi sli~na aktivnost albumina (AlbEst) bila je zna~ajno (P<0,05) sni`ena (34%) kod obolelih u pore|enju sa kontrolom (47%). Zna~ajan porast LDH-5 i pad LDH-1 i -2 izoenzima tako|e su prime}eni Summary: The aim of the present study was to assess the serum proteins and enzymes level using polyacrylamide gel electrophoretic (PAGE) profiles in human subjects with osteo arthritis (OA). Forty-one subjects with confir med OA were selected for the present study. Sera were collected from these individuals and loaded in equal amounts on native and denaturing PAGE separately. Software analysis of these profiles was done using Scion Imaging (Beta release-4, Scion Corporation) and GelPro (Media Cybernetics, USA) programs. To visualize esterases (Est) and lactate dehydro genase (LDH) isoenzymes in the sera of these patients substrate specific staining was performed. Differences in the values of control and OA subjects were tested statistically. Software analysis of native-PAGE profiles revealed the presence of nineteen peptides in control and twenty one in OA subjects respectively. Two extra peptides were present in the b-globulins region of OA subjects. Significant decline from 42.77% to 34.72% in albumin levels (hypoalbuminemia) was observed in OA subjects with total albumin to globulin ratio 0.58. In SDS-PAGE, the difference in control and OA subjects was observed among eight peptides with molecular weight 25, 22 and 20 kDa (absent in OA) and five novel peptides 270, 125, 30, 21.36 and 18.4 kDa (absent in controls), while albumin retains the major activity. For enzymes...

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Analysis of Serum Proteins and Enzymes Level in Human Subjects with Osteoarthritis

Ahmad¹,

Irshad²,

Ahmad³

et al. 2011

Journal of Medical Biochemistry

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“…Similarly, early reports implied that DJD might not alter enzyme activities in SF (Cohen 1964). However, later studies in horses and humans unanimously demonstrated an overall increase in synovial LDH activity in conjunction with a number of inXammatory and non-inXammatory joint diseases, such as infectious arthritis, rheumatoid arthritis, gout, and osteochondrosis dissecans (Lindy et al 1971;Messieh 1996;Pejovic et al 1992;Rejno 1976;Yancik et al 1987). As for the dog, it has been shown recently that LDH activity in SF is likely to correlate with the extent of degenerative lesions, thus making LDH a potentially useful and speciWc marker for DJD (Hurter et al 2005;Schmöckel et al 2001).…”

Section: Discussionmentioning

confidence: 95%

Distribution of lactate dehydrogenase in healthy and degenerative canine stifle joint cartilage

Walter

Spreng

Schmöckel

et al. 2007

Histochem Cell Biol

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In dogs, degenerative joint diseases (DJD) have been shown to be associated with increased lactate dehydrogenase (LDH) activity in the synovial fluid. The goal of this study was to examine healthy and degenerative stifle joints in order to clarify the origin of LDH in synovial fluid. In order to assess the distribution of LDH, cartilage samples from healthy and degenerative knee joints were investigated by means of light and transmission electron microscopy in conjunction with immunolabeling and enzyme cytochemistry. Morphological analysis confirmed DJD. All techniques used corroborated the presence of LDH in chondrocytes and in the interterritorial matrix of healthy and degenerative stifle joints. Although enzymatic activity of LDH was clearly demonstrated in the territorial matrix by means of the tetrazolium-formazan reaction, immunolabeling for LDH was missing in this region. With respect to the distribution of LDH in the interterritorial matrix, a striking decrease from superficial to deeper layers was present in healthy dogs but was missing in affected joints. These results support the contention that LDH in synovial fluid of degenerative joints originates from cartilage. Therefore, we suggest that (1) LDH is transferred from chondrocytes to ECM in both healthy dogs and dogs with degenerative joint disease and that (2) in degenerative joints, LDH is released from chondrocytes and the ECM into synovial fluid through abrasion of cartilage as well as through enhanced diffusion as a result of increased water content and degradation of collagen.

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In vivo expression of osteogenic markers and bone mineral density at the surface of fluoride-modified titanium implants

et al. 2008

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Levels of lactate dehydrogenase in osteoarthritic and failed total knee joints

Cited by 5 publications

References 1 publication

Analysis of Serum Proteins and Enzymes Level in Human Subjects with Osteoarthritis

Analysis of Serum Proteins and Enzymes Level in Human Subjects with Osteoarthritis

Distribution of lactate dehydrogenase in healthy and degenerative canine stifle joint cartilage

In vivo expression of osteogenic markers and bone mineral density at the surface of fluoride-modified titanium implants

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