Levels of nitric oxide metabolites, adiponectin and endothelin are associated with SNPs of the adiponectin and endothelin genes

Abstract

Adiponectin, endothelin and nitric oxide (NO) are major regulators of vascular function. An imbalance of vasoactive factors contributes to the onset and progression of atherosclerosis. Various single nucleotide polymorphisms (SNPs) are considered to be risk factors for coronary heart disease. However, the molecular mechanisms of their associations with the components of endothelial dysfunction are poorly understood. In the present study, rs17366743, rs17300539, rs266729, rs182052 and rs2241766 SNPs of the adiponectin (ADIPOQ) gene and rs2070699, rs1800542 and rs1800543 SNPs of the endothelin-1 (EDN1) gene were genotyped in 477 patients with coronary heart disease who were subjected to coronary angiography, in order to determine the presence or absence of coronary atherosclerosis. The serum levels of adiponectin, endothelin and stable metabolites of NO, (nitrate and nitrite NOx), were assayed and their associations with the SNP genotypes and coronary lesions were calculated. The results indicated that rs17366743 of the ADIPOQ gene and rs2070699 and rs1800543 of the EDN1 gene were associated with the levels of NOx in women, which in turn was associated with cardiovascular mortality. In men, rs182052 and rs266729 of the ADIPOQ gene were associated with adiponectin levels, whereas rs17366743 of the ADIPOQ gene was associated with endothelin levels. Additionally, these SNPs were indirectly associated with the prevalence of coronary lesions in men. Therefore, the tested SNPs can be considered potential risk factors that lead to imbalance of vasoactive mediators in a gender-specific manner and contribute to the development of clinical manifestations of atherosclerosis.

Association of AdipoQ gene variation (rs1501299) and oxidative stress with cardiovascular disease in North West Indian population of Punjabi women

“…Because, superoxide anion which is the main oxygen species reacts with nitric oxide radical forming peroxynitrite -a free radical, causing oxidative stress and cellular damage. Gumanova et al (24) in their study found that higher levels of NO are significantly associated with coronary lesions though, some studies reported increased activity of SOD in ischemic heart disease patients (25). On the other hand, other studies similar to our study revealed decreased activity of SOD (19,21,26).…”

Single nucleotide polymorphisms of ADIPOQ gene associated with cardiovascular disease risk factors in European adolescents: the Healthy Lifestyle in Europe by Nutrition in Adolescence study

“…In addition, intron-retention (not removed introns) has recently garnered attention as a major component of the global alternative splicingmediated regulation and mRNA expression regulation, which are part of many mammalian programs of gene expression [44]. Indeed, Gumanova et al [45] reported the association between rs182052 and rs266729 ADIPOQ polymorphisms with adiponectin levels in men. Overall, these lines of evidence suggest that ADIPOQ polymorphisms may alter the expression levels and consequently adiponectin content.…”

Valid cardiac biomarkers. Part I

“…Установлено, что высокая концентрация NOx >75 мкМнезависимый ФР сердечно-сосудистой смерти в течение 3-летнего периода наблюдения [65], ФР сердечно-сосудистой смерти в течение 8-летнего периода наблюдения у мужчин, и за этот же период -не связанный с полом ФР смерти от всех причин [66]. Полученные результаты согласуются с опубликованными позднее данными Фремингемского исследования [67], собственными данными авторов [68] и данными японских исследователей [69]. Кроме того, было обнаружено, что высокий уровень NOx ассоциирован с большим количеством хронических неинфекционных заболеваний, за исключением онкологических [70].…”