Leveraging the third dimension in microfluidic devices using 3D printing: no longer just scratching the surface

Pradela‐Filho, Lauro A.; Paixão, Thiago R.L.C.; Nordin, Gregory P.

doi:10.1007/s00216-023-04862-w

Cited by 8 publications

(2 citation statements)

References 31 publications

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“…First, 3D microfluidic channels with complex shapes and architectures could now be built in a single step, compared to the regular 2.5D fabrication methods (soft lithography mouldings, layer-by-layer taping, silicon etching). 3 Moreover, prototyping time is also strongly reduced, down to a few hours from the chip design to the end product. For large printer, the footprint is exceeding 200 cm 2 , thus allowing the fabrication of large chips or the batch manufacturing of numerous chips at once.…”

Section: Introductionmentioning

confidence: 99%

Functionality integration in stereolithography 3D printed microfluidics using a “print-pause-print” strategy

Sagot,

Derkenne,

Giunchi

et al. 2024

Lab Chip

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Section: Introductionmentioning

confidence: 99%

Functionality integration in stereolithography 3D printed microfluidics using a “print-pause-print” strategy

Sagot,

Derkenne,

Giunchi

et al. 2024

Lab Chip

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“…Its appeal is driven in part by capabilities for rapid prototyping, device miniaturization, printing complex structures with high resolution, component integration, and ease of use. Moreover, unlike conventional microfluidic device fabrication technologies, 3D printing can utilize all three dimensions of the device volume for component geometry, placement, and interconnect routing [ 7 , 8 , 9 , 10 , 11 ]. Our group has developed several custom 3D printers and tools based on Digital Light Processing Stereolithography (DLP-SLA), along with optimized resins, to facilitate printing high-resolution microfluidic devices [ 12 , 13 , 14 , 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Biocompatible High-Resolution 3D-Printed Microfluidic Devices: Integrated Cell Chemotaxis Demonstration

et al. 2023

Self Cite

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We demonstrate a method to effectively 3D print microfluidic devices with high-resolution features using a biocompatible resin based on avobenzone as the UV absorber. Our method relies on spectrally shaping the 3D printer source spectrum so that it is fully overlapped by avobenzone’s absorption spectrum. Complete overlap is essential to effectively limit the optical penetration depth, which is required to achieve high out-of-plane resolution. We demonstrate the high resolution in practice by 3D printing 15 μm square pillars in a microfluidic chamber, where the pillars are separated by 7.7 μm and are printed with 5 μm layers. Furthermore, we show reliable membrane valves and pumps using the biocompatible resin. Valves are tested to 1,000,000 actuations with no observable degradation in performance. Finally, we create a concentration gradient generation (CG) component and utilize it in two device designs for cell chemotaxis studies. The first design relies on an external dual syringe pump to generate source and sink flows to supply the CG channel, while the second is a complete integrated device incorporating on-chip pumps, valves, and reservoirs. Both device types are seeded with adherent cells that are subjected to a chemoattractant CG, and both show clear evidence of chemotactic cellular migration. Moreover, the integrated device demonstrates cellular migration comparable to the external syringe pump device. This demonstration illustrates the effectiveness of our integrated chemotactic assay approach and high-resolution biocompatible resin 3D printing fabrication process. In addition, our 3D printing process has been tuned for rapid fabrication, as printing times for the two device designs are, respectively, 8 and 15 min.

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Challenges faced with 3D-printed electrochemical sensors in analytical applications

Pradela‑Filho,

Araújo,

Ataide

et al. 2024

Anal Bioanal Chem

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Leveraging the third dimension in microfluidic devices using 3D printing: no longer just scratching the surface

Cited by 8 publications

References 31 publications

Functionality integration in stereolithography 3D printed microfluidics using a “print-pause-print” strategy

Functionality integration in stereolithography 3D printed microfluidics using a “print-pause-print” strategy

Biocompatible High-Resolution 3D-Printed Microfluidic Devices: Integrated Cell Chemotaxis Demonstration

Challenges faced with 3D-printed electrochemical sensors in analytical applications

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