Lewinella aurantiaca sp. nov., a carotenoid pigment-producing bacterium isolated from surface seawater

Abstract

A Gram-stain-negative, aerobic, rod-shaped, carotenoid-pigmented, motile-by-gliding bacterium, which was designated as SSH13T, was isolated from a surface seawater sample collected from Sehwa Beach in the Republic of Korea. Strain SSH13T was oxidase-negative, catalase-positive and grew at 2–37 °C (optimum, 30 °C), in the presence of 0.5–6% NaCl and within a pH range of pH 6–10 (optimum, pH 8). The novel isolate required NaCl for growth and grew optimally with approximately 2 % NaCl. Chemotaxonomic and morphological characteristics were consistent with members of the genus Lewinella . Furthermore, phylogenetic analysis based on 16S rRNA gene sequencing revealed that strain SSH13T was most closely related to the type strains of the genus Lewinella . Strain SSH13T had highest 16S rRNA gene sequence similarities to Lewinella persica DSM 23188T (95.3 %) and Lewinella agarilytica KCTC 12774T (95.0 %). The major fatty acids of SSH13T were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and iso-C15 : 0. Strain SSH13T contained phosphatidylethanolamine as a major polar lipid. Menaquinone-7 was the predominant respiratory quinone. The average nucleotide identity values between strain SSH13T and L. persica T-3T and L. agarilytica SST-19T were 72.9 and 72.6 %, respectively. The DNA G+C content of the genomic DNA was 52.8 mol%. The present study aimed to determine the multiple-antibiotic resistance of the novel bacterium. Based on phylogenetic and phenotypic analyses, strain SSH13T is considered to represent a novel species of the genus Lewinella , for which the name Lewinella aurantiaca sp. nov. (type strain SSH13T=KACC 21167T=NBRC 113866T) is proposed.

Chryseobacterium caseinilyticum sp. nov., a casein hydrolyzing bacterium isolated from rice plant and emended description of Chryseobacterium piscicola

“…GCR10 T was only resistant to erythromycin and cefazolin and susceptible to all of the other antibiotics, however the reference strain Chryseobacterium piscicola VQ-6316s T was susceptible to all antibiotics. This antibiotic-resistant novel bacteria could be used as a potential agent for bioremediation of antibiotic-polluted environments and to clean water ecosystems by biodegradation of antibiotics by a biotic process as described previously [51].…”

Pontibacter cellulosilyticus sp. nov., a carboxymethyl cellulose-hydrolysing bacterium isolated from coastal water

“…Strain SD6 T and its reference strains were cultured and extracted for carotenoid analysis. Carotenoids were extracted using a 10 ml methanol/acetone (1 : 1, v/v) mixture and the absorption spectrum was evaluated with a spectrophotometer (Multiskan GO; Thermo Fisher Scientific) [45].…”

Nocardioides donggukensis sp. nov. and Hyunsoonleella aquatilis sp. nov., isolated from Jeongbang Waterfall on Jeju Island

“…Isoprenoid quinones were extracted using chloroform and methanol at a ratio of 2 : 1 (v/v) and were analysed using high-performance lipid chromatography by following a previously published method [40,41]. Cellular extracts for carotenoid analysis were prepared with a 10 ml methanolacetone (v/v, 1 : 1) mixture [42], and the absorption spectrum of the carotenoid pigments were judged using a spectrophotometer (Multiskan GO, Thermo Fisher Scientific). The presence of flexirubin-type pigments was investigated using 20 % KOH solution (w/v) [43].…”