LIBS-Based Detection of Antioxidant Elements in Seeds of Emblica officinalis

Abstract

The aim of the study was to determine the effect of the elements of the extract of seed from Emblica officinalis on antioxidant enzymes and osmotic fragility of erythrocytes membrane in normal as well as streptozotocininduced severely diabetic albino Wister rats. The results revealed that the untreated diabetic rats exhibited increase in oxidative stress as indicated by significantly diminished activities of free radical scavenging enzymes such as catalase (CAT) and superoxide dismutase (SOD) by 37.5% (p < 0.001) and 18.6% (p < 0.01), respectively. However, the E. officinalis seed extract treatment showed marked improvements in CAT and SOD activities by 47.09% (p<0.001) and 21.61% (p<0.001), respectively. The enhanced lipid peroxidation by 30.87% (p<0.001) in erythrocytes of untreated diabetic rats was significantly accentuated in the extract treated animals by 23.72% (p<0.001). The erythrocytes showed increased osmotic fragility due to diabetes in terms of hemolysis. It attained the normal level in diabetic treated group. The findings thus suggest that E. officinalis seed extract has the potential to be exploited as an agent to boost the antioxidant system in the diabetic animal model. Laser-induced breakdown spectroscopy has been used as an analytical tool to detect major and minor elements like Mg, Fe, Na, K, Zn, Ca, H, O, C, and N present in the extract. The higher concentration of Ca (II), Mg (II) and Fe (II) as reflected by their intensities are responsible for the antioxidant potential of E. officinalis.

Antidyslipidemic Effect of Ocimum sanctum Leaf Extract in Streptozotocin Induced Diabetic Rats

“…Some of the main constituents of leaf of O. sanctum are phenolic compounds, flavonoids, sesquiterpenes and monoterpenes, glycosides, Steroids, sterols as well as volatile and non volatile, (Fixed) oils [2,3]. It is suggested that all or some of these bioactive compounds may be responsible for hypoglycemic, antidyslipidemic and antioxidant effects of the medicinal plants [22,23]. Kelm et al [24,25] found that leaves and stems of Ocimum sanctum contains cirsilineol, cirsimaritin, isothymusin, isothymonin, apigenin, rosmarinic acid, and appreciable quantities of eugenol and all these are known to possess potent lipid lowering and antioxidant activities.…”

Anticoagulant, Antiherpetic and Antibacterial Activities of Sulphated Polysaccharide from Indian Medicinal Plant Tridax procumbens L. (Asteraceae)

“…Mehta et al. [1] reported antioxidant elements from seeds of Emblica officinalis as detected by laser-induced breakdown spectroscopy. It was found that E. officinalis extract effected antioxidant enzymes and has the potential acted as an agent to boost the antioxidant system in the diabetic animal model.…”

Role of Spectral Studies in Detection of Antibacterial Phytoelements and Phytochemicals of Moringa oleifera

“…This activity includes studies of laser ablation and properties of the laser spark, the development of methods to enhance LIBS detection capabilities, and the application of LIBS to specific analysis needs. Recently Watal's research group has been successfully applied LIBS technique for the analysis of trace elements in medicinal plants [6][7][8][9] , responsible for their specific bioactivities.…”