The aim of the study was to determine the effect of the elements of the extract of seed from Emblica officinalis on antioxidant enzymes and osmotic fragility of erythrocytes membrane in normal as well as streptozotocininduced severely diabetic albino Wister rats. The results revealed that the untreated diabetic rats exhibited increase in oxidative stress as indicated by significantly diminished activities of free radical scavenging enzymes such as catalase (CAT) and superoxide dismutase (SOD) by 37.5% (p < 0.001) and 18.6% (p < 0.01), respectively. However, the E. officinalis seed extract treatment showed marked improvements in CAT and SOD activities by 47.09% (p<0.001) and 21.61% (p<0.001), respectively. The enhanced lipid peroxidation by 30.87% (p<0.001) in erythrocytes of untreated diabetic rats was significantly accentuated in the extract treated animals by 23.72% (p<0.001). The erythrocytes showed increased osmotic fragility due to diabetes in terms of hemolysis. It attained the normal level in diabetic treated group. The findings thus suggest that E. officinalis seed extract has the potential to be exploited as an agent to boost the antioxidant system in the diabetic animal model. Laser-induced breakdown spectroscopy has been used as an analytical tool to detect major and minor elements like Mg, Fe, Na, K, Zn, Ca, H, O, C, and N present in the extract. The higher concentration of Ca (II), Mg (II) and Fe (II) as reflected by their intensities are responsible for the antioxidant potential of E. officinalis.

Gallstones obtained from patients from the north-east region of India (Assam) were studied using laser-induced breakdown spectroscopy (LIBS) technique. LIBS spectra of the different layers (in cross-section) of the gallstones were recorded in the spectral region 200-900 nm. Several elements, including calcium, magnesium, manganese, copper, silicon, phosphorus, iron, sodium and potassium, were detected in the gallstones. Lighter elements, including carbon, hydrogen, nitrogen and oxygen were also detected, which demonstrates the superiority of the LIBS technique over other existing analytical techniques. The LIBS technique was applied to investigate the evolution of C(2) swan bands and CN violet bands in the LIBS spectra of the gallstones in air and an argon atmosphere. The different layers (dark and light layers) of the gallstones were discriminated on the basis of the presence and intensities of the spectral lines for carbon, hydrogen, nitrogen, oxygen and copper. An attempt was also made to correlate the presence of major and minor elements in the gallstones with the common diet of the population of Assam.

This paper reports, the Laser Induced Breakdown Spectroscopy (LIBS) studies and structure elucidation of compounds isolated from the fruit extract of Moringa oleifera and also deals with their possible effects on some bacterial strains viz. Staphylococcus aureus, Klebsiella pneumonia, Escherichia coli and Pseudomonas aeruginosa. The extract was found to be active against all four microorganisms used. Extent of inhibitory effect of extract was assessed at different concentrations of 25, 50, 75 mg/ ml by measuring diameter of inhibition zone (DIZ). Our results clearly showed that the 75 mg/ml concentration of the extract had 14, 12 and 18 mm of the DIZ against Staphylococcus aureus, Klebsiella pneumonia and Pseudomonas aeruginosa and 14 mm with 50 mg/ml concentration against Escherichia coli. The results were compared with the standard antibiotic 'ampicillin' of 1 mg/ml concentration. LIBS was recorded with high power pulsed laser beam from Nd: YAG Laser (Continuum Surelite III-10), focused on the surface of the material, which was in liquid form, to generate plasma on the surface of the sample. LIBS data clearly demonstrate the presence of trace elements, magnesium and iron, in high concentration in the extract. Whereas, from the phytochemical profile reveals the presence of two new compounds, S-ethyl-N-{4-[(α-Lrhamnosyloxy) benzyl]} thiocarbamate and 2-acetoxy {4-[(2′,3′,4′-tri-O-acetyl-α-L-rhamnosyloxy) benzyl]} acetonitrile as the major constituents. This study is the first report on synergetic effect of the phytoconstituents and certain set of elements present in their defined role in bacterial management against different bacterial strains.

We report a case of rapidly progressive vision loss in a young woman with a history of injection drug use. Subsequent enucleation of the affected eye was done and Bacillus cereus grew on tissue culture. B. cereus is a rare cause of endogenous endophthalmitis due to hematogenous seeding of the vitreous in the setting of injection drug use.

Background: Finger millet being highly nutritious and easy to digest is one of the important crops grown in Tamil Nadu, Karnataka, and Andhra Pradesh. Organic farming is gaining importance among the farmers with increasing yield, improving the quality of grains, and maintaining soil quality. Since the land availability is decreasing with increasing population. Finger millet has great scope under organic cultivation which could result in increased soil condition. Methodology: A field trial was performed at Agricultural College and Research Institute, Killikulam, Tuticorin, TNAU, Tamil Nadu from 2019 to 2020 during the rabi season to evaluate the performance of various organic sources concerning the soil properties changes. The study was conducted with an RBD design. Treatments include solid manures and liquid organics combination and their performance was evaluated. Finger millet - Co (Ra) 15 was used as a test variety. Soil health represents the soil properties generally including pH, EC, organic carbon content, N, P, and K levels, and also the soil microbial population. Results and Discussion: From the study, it was found that the application of 100 percent of nitrogen through poultry with foliar application of panchagavya at 30 and 45 DAT gave better yield and also enriched the soil fertility status. Conclusion: 100% N through Poultry manure and 3% panchagavya foliar spray at 30 & 45 DAT in transplanted finger millet gave increased soil health with higher soil properties. Thus, Organic farming proved to be an eco-friendly management option in finger millet for maintaining good soil health.