Licht- und elektronenmikroskopische Untersuchungen am Subcommissuralorgan der Maus

The House Mouse

“…The nomenclature of inbred strains is explained in J Heredity 54,[159][160][161][162]1963, and in Green (2}. Procedure Animals were placed in a room in which the normal light-dark sequence was reversed for at least 4 days. Usually first litters were used, but a few second litters were included.…”

The subcommissural organ

“…6,10,11). They display an infolded nucleus with abundant euchromatin and are located in the basal cell region (Herrlinger, 1970;Stanka et al, 1964). The cell body presents a clear zonation; the following cytoplasmic regions may be recognized: perinuclear, intermediate, subapical, and apical.…”

“…Transmission and scanning electron microscopy show that each ependymal cell projects a large protrusion into the ventricle (Herrlinger, 1970;Krstic, 1975;Stanka et al, 1964;Weindl and Schinko, 1975). The cells display a few cilia and numerous mitochondria (Leonhardt, 1980).…”

“…The most distinct ultrastructural feature of the SCO of virtually all species is the presence of large and dilated cisternae of the rough endoplasmic reticulum (RER), which may reach a diameter of 7 µm (Herrlinger, 1970;Stanka et al, 1964). Only about 2-5% of the surface area of these cisternae is studded with ribosomes, indicating that in each cisternum the rate of synthesis is low.…”

Tissue culture of bovine subcommissural organ

“…In view of the complete demonstration in the present study of immunoreactivity in all structures involved in the secretory pathway of RF proteins, and especially in the numerous secretory vesicles, and additionally in the large amount of extracellularly located secretory material, it should be accepted as evident that bovine SCO cells secrete actively in vitro. No direct release of secretory material was observed from endoplasmic cisterns avoiding Golgi passage as discussed by Stanka et al (1964), Herrlinger (1970), Rodriguez (1970), Chen et al (1973), Schneider (1972, and L6secke et al (1984) as a second secretory pathway.…”