Lifespan-extending property of 6-shogaol from Zingiber officinale Roscoe in Caenorhabditis elegans

Abstract

Aging is a key risk factor for many diseases, understanding the mechanism of which is becoming more important for drug development given the fast-growing aging population. In the course of our continued efforts to discover anti-aging natural products, the active constituent 6-shogaol was isolated from Zingiber officinale Roscoe. The chemical structure of 6-shogaol was identified by comparison of its NMR data with literature values. The lifespan-extending effect of 6-shogaol was observed in a dose-dependent manner in Caenorhabditis elegans that has been widely used as a model organism for human aging studies. Mechanism of such action was investigated using C. elegans models, suggesting that 6-shogaol is capable of increasing stress tolerances via enzyme induction. The proposed mechanism was further supported by observation of the increase in SOD and HSP expressions upon treatment with 6-shogaol in transgenic strains of C. elegans which contain GFP-based reporters. In addition, the mechanism was elaborated by confirming that the effect observed for 6-shogaol is independent from other aging-related factors that are known to affect the aging process of C. elegans.

Role of Garlic and Ginger in Anti-oxidative and Anti-inflammatory Effects in Aging

“…Other than its anti-oxidative, anti-inflammatory, and anti-carcinogenic activities, it also has lifespan-extending property [8][9][10][11]. Hence, this paper attempts to review the current knowledge of biologically available natural active components of garlic and ginger and their physiological roles against aging process.…”

Antioxidant and Lifespan-Extending Effects of a Rejuvenating Thai Traditional Polyherbal Remedy (Phy-Blica-O) in Caenorhabditis elegans

“…The extract slightly reduced the accumulation of lipofuscin and enhanced the level of SOD production of C. elegans. Similarly, an increase in the mean lifespan of C. elegans was observed when treated with ellagic acid 52 , Allium sativum 53 , and Zingiber officinale 54 . Even though naringenin failed to extend the lifespan of C. elegans 55 , it was highlighted as a promising hepatoprotective agent that could decrease oxidative biomarkers, such as levels of lipid peroxidation and protein carbonylation, and increase the status of antioxidant defenses in other animal models.…”

Metabolites Produced by the Oral Commensal Bacterium Corynebacterium durum Extend the Lifespan of Caenorhabditis elegans via SIR-2.1 Overexpression

“…The maximum increases observed upon treatment with PEA and N-APEA were 21.6 and 19.9%, respectively, at 100 µM. Interestingly, unlike other anti-aging natural products that we have encountered [23,24], the six compounds failed to show DPPH radical scavenging activities ( Figure S8).…”

“…The six compounds analogous to the major metabolites of C. durum were inactive in DPPH radical scavenging assays ( Figure S8), showing an inconsistency with the biological properties of anti-aging natural products encountered in our former studies [23,24]. Therefore, expression of the proteins known to be involved in the regulation of lifespan, such as the evolutionarily conserved protein SIR-2.1, were hypothesized to be the underlying mechanism of the lifespan extension induced by PEA and N-APEA.…”