Metabolites Produced by the Oral Commensal Bacterium Corynebacterium durum Extend the Lifespan of Caenorhabditis elegans via SIR-2.1 Overexpression

Kim, Jun Hyeong; Bang, In Hyuk; Noh, Yun Jeong; Kim, Dae Keun; Bae, Eun Ju; Hwang, In Hyun

doi:10.3390/ijms21062212

Cited by 12 publications

(9 citation statements)

References 35 publications

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“…Recent studies have found C. matruchotii and C. durum associated with health rather than caries [53][54][55]. Metabolites produced by C. durum have also been found to extend lifespan in the model organism Caenorhabditis elegans [56] and C. durum elicited no inflammatory response from human gingival and oral mucosal cells, suggesting it is a commensal [57]. S. cristatus has been shown to inhibit biofilm formation of the periodontal pathogen P. gingivalis by repression of virulence genes [58,59].…”

Section: Discussionmentioning

confidence: 99%

Corncob structures in dental plaque reveal microhabitat taxon specificity

et al. 2022

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Background The human mouth is a natural laboratory for studying how bacterial communities differ across habitats. Different bacteria colonize different surfaces in the mouth—teeth, tongue dorsum, and keratinized and non-keratinized epithelia—despite the short physical distance between these habitats and their connection through saliva. We sought to determine whether more tightly defined microhabitats might have more tightly defined sets of resident bacteria. A microhabitat may be characterized, for example, as the space adjacent to a particular species of bacterium. Corncob structures of dental plaque, consisting of coccoid bacteria bound to filaments of Corynebacterium cells, present an opportunity to analyze the community structure of one such well-defined microhabitat within a complex natural biofilm. Here, we investigate by fluorescence in situ hybridization and spectral imaging the composition of the cocci decorating the filaments. Results The range of taxa observed in corncobs was limited to a small subset of the taxa present in dental plaque. Among four major groups of dental plaque streptococci, two were the major constituents of corncobs, including one that was the most abundant Streptococcus species in corncobs despite being relatively rare in dental plaque overall. Images showed both Streptococcus types in corncobs in all individual donors, suggesting that the taxa have different ecological roles or that mechanisms exist for stabilizing the persistence of functionally redundant taxa in the population. Direct taxon-taxon interactions were observed not only between the Streptococcus cells and the central corncob filament but also between Streptococcus cells and the limited subset of other plaque bacteria detected in the corncobs, indicating species ensembles involving these taxa as well. Conclusions The spatial organization we observed in corncobs suggests that each of the microbial participants can interact with multiple, albeit limited, potential partners, a feature that may encourage the long-term stability of the community. Additionally, our results suggest the general principle that a precisely defined microhabitat will be inhabited by a small and well-defined set of microbial taxa. Thus, our results are important for understanding the structure and organizing principles of natural biofilms and lay the groundwork for future work to modulate and control biofilms for human health.

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Section: Discussionmentioning

confidence: 99%

Corncob structures in dental plaque reveal microhabitat taxon specificity

et al. 2022

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“…They play critical roles in regulating cellular metabolism, responses to oxidative stress, and lifespan through the deacetylation of histones and non-histones. 27 One report validated that TGS enhanced the resistance to oxidative stress and improved the mitochondrial functions through the activation of SIRT 1 in vitro. 28 SIR 2.1, the worm sirtuin homolog, is a protein deacetylase that depends on the cellular coenzyme nicotinamide adenine dinycleotied (NAD + ).…”

Section: Tgs Required Nrf2/skn-1 Activation To Extend the Lifespan Of C Elegansmentioning

confidence: 93%

Ginsenoside extract from ginseng extends lifespan and health span inCaenorhabditis elegans

et al. 2021

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“…Based upon our results, it appears as if there is either no active immunosuppressive mechanism employed by C. durum or this ability is regulated, since it would otherwise have been expected to function in each of the multispecies biofilm conditions. C. durum has not been investigated for its ability to modulate the immune response, but it has been shown to increase the longevity of Caenorhabditis elegans via the secretion of monoamines and N -acetyl monoamines ( Kim et al., 2020 ), suggesting that this species can influence the health of the host, as would be expected from a true commensal organism. We also previously demonstrated how S. sanguinis phagocytosis is inhibited by coculture with C. durum ( Treerat et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Differential Response of Oral Mucosal and Gingival Cells to Corynebacterium durum, Streptococcus sanguinis, and Porphyromonas gingivalis Multispecies Biofilms

Redanz

Treerat

Prakasam

et al. 2021

Front. Cell. Infect. Microbiol.

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Polymicrobial interactions with oral mucosal surfaces determine the health status of the host. While a homeostatic balance provides protection from oral disease, a dysbiotic polymicrobial community promotes tissue destruction and chronic oral diseases. How polymicrobial communities transition from a homeostatic to a dysbiotic state is an understudied process. Thus, we were interested to investigate this ecological transition by focusing on biofilm communities containing high abundance commensal species and low abundance pathobionts to characterize the host-microbiome interactions occurring during oral health. To this end, a multispecies biofilm model was examined using the commensal species Corynebacterium durum and Streptococcus sanguinis and the pathobiont Porphyromonas gingivalis. We compared how both single and multispecies biofilms interact with different oral mucosal and gingival cell types, including the well-studied oral keratinocyte cell lines OKF4/TERT-1and hTERT TIGKs as well as human primary periodontal ligament cells. While single species biofilms of C. durum, S. sanguinis, and P. gingivalis are all characterized by unique cytokine responses for each species, multispecies biofilms elicited a response resembling S. sanguinis single species biofilms. One notable exception is the influence of P. gingivalis upon TNF-α and Gro-α production in hTERT TIGKs cells, which was not affected by the presence of other species. This study is also the first to examine the host response to C. durum. Interestingly, C. durum yielded no notable inflammatory responses from any of the tested host cells, suggesting it functions as a true commensal species. Conversely, S. sanguinis was able to induce expression and secretion of the proinflammatory cytokines IL-6 and IL-8, demonstrating a much greater inflammatory potential, despite being health associated. Our study also demonstrates the variability of host cell responses between different cell lines, highlighting the importance of developing relevant in vitro models to study oral microbiome-host interactions.

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Metabolites Produced by the Oral Commensal Bacterium Corynebacterium durum Extend the Lifespan of Caenorhabditis elegans via SIR-2.1 Overexpression

Cited by 12 publications

References 35 publications

Corncob structures in dental plaque reveal microhabitat taxon specificity

Corncob structures in dental plaque reveal microhabitat taxon specificity

Ginsenoside extract from ginseng extends lifespan and health span inCaenorhabditis elegans

Differential Response of Oral Mucosal and Gingival Cells to Corynebacterium durum, Streptococcus sanguinis, and Porphyromonas gingivalis Multispecies Biofilms

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