We present lifetime measurements of the 7S 1/2 level and the 6p manifold of Rb. We use a time-correlated single-photon counting technique on a sample of 85 Rb atoms confined and cooled in a magneto-optical trap. The upper state of the 5P 1/2 repumping transition serves as the resonant intermediate level for two-photon excitation of the 7s level. A probe laser provides the second step of the excitation, and we detect the decay of the atomic fluorescence to the 5P 3/2 level at 741 nm. The decay process feeds the 6p manifold which decays to the 5s ground state emitting uv photons. We measure lifetimes of 88.07 ± 0.40 ns and 120.7 ± 1.2 ns for the 7S 1/2 level and 6p manifold, respectively; while the hyperfine splitting of the 7S 1/2 level is 282.6 ± 1.6 MHz.The agreement with theoretical calculations confirms the understanding of the wavefunctions involved, and provides confidence on the possibility of extracting weak interaction constants from a Parity Non-Conservation measurement.