Ligand Assisted Stabilization of Fluorescence Nanoparticles; an Insight on the Fluorescence Characteristics, Dispersion Stability and DNA Loading Efficiency of Nanoparticles

Abstract

This work reports on the ligand assisted stabilization of Fluospheres® carboxylate modified nanoparticles (FCMNPs), and subsequently investigation on the DNA loading capacity and fluorescence response of the modified particles. The designed fluorescence bioconjugate was characterized with enhanced fluorescence characteristics, good stability and large surface area with high DNA loading efficiency. For comparison purpose, bovine serum albumin (BSA) and polyethylene glycol (PEG) with three different length strands were used as cross linkers to modify the particles, and their DNA loading capacity and fluorescence characteristics were investigated. By comparing the performance of the particles, we found that the most improved fluorescence characteristics, enhanced DNA loading and high dispersion stability were obtained, when employing PEG of long spacer arm length. The designed fluorescence bioconjugate was observed to maintain all its characteristics under varying pH over an extended period of time. These types of bioconjugates are in great demand for fluorescence imaging and in vivo fluorescence biomedical application, especially when most of the as synthesized fluorescence particles cannot withstand to varying in vivo physiological conditions with decreases in fluorescence response and DNA loading efficiency.

Designed Strategies for Fluorescence-Based Biosensors for the Detection of Mycotoxins

“…To overcome technical obstacles of organic dyes, nanomaterials—like semiconductor quantum dots [ 18 , 19 ], upconversion nanoparticles [ 20 ], and organic polymers nanoparticles—have been explored as excellent alternatives [ 21 ].…”

Nanomaterial-based fluorescent biosensor for veterinary drug detection in foods

“…Fluorescence biosensors as the most exploited for food contaminants detection, due to its high sensitivity, wide dynamic range and versatile detection methods [ 22 ]. A diverse range of nanomaterial-based fluorophores are often brighter (higher fluorescence quantum yield), more stable, and sometimes have sharper emission peaks compared to organic fluorophores, allowing highly sensitive and multiplexed detection [ 23 ]. In addition, nanomaterials were used as immobilization platforms due to their unique features, such as a high specific surface area, and surfaces that can be readily modified with ligands.…”