Ligation of porcine Fc gamma receptor III inhibits levels of antiviral cytokine in response to PRRSV infection in vitro

Zhang, Liujun; Chen, Jing; Wang, Dongmei; Li, Nana; Qin, Yunjie; Du, Dongying; Yang, Mingfan; Xia, Pingan

doi:10.1016/j.rvsc.2016.01.009

Cited by 8 publications

(6 citation statements)

References 49 publications

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“…The mechanism of ADE caused by PRRSV infection involves several cell-surface molecules, including the FcRs (Halstead and O'Rourke 1977), complement receptors, CD4/15/33 (Novakovic et al 2016;Yuste et al 2017) and b 2 -microglobulin (Tirado and Yoon 2003;Bao et al 2013). The antibody-FccR interaction is known to play an important role in the ADE phenomenon (Zhang et al 2016). The suggestion that FccR can mediate ADE in dengue virus infection was first made in 1977 (Halstead and O'Rourke 1977).…”

Section: Discussionmentioning

confidence: 99%

“…The polyclonal antibody against FceRI was derived from serum of rabbit immunized with extracellular recombinant GST-FceRI proteins coupled with mineral oil adjuvant as previously described (Zhang et al 2016).…”

Section: Generation and Characterization Of Antibodiesmentioning

confidence: 99%

“…Proinflammatory cytokines (TNF-a, IL-1 and IL-6) are induced after infection by PRRSV (Nedumpun et al 2017). PRRSV tends to attack the lungs and lymphoid organs and can proliferate in a variety of cells, such as porcine alveolar macrophages (PAMs), bone marrow-derived dendritic cells (BMDCs) and MARC-145 cells (Duan et al 1997;Gao et al 2016;Zhang et al 2016). As the major host cell, alveolar macrophages expressing many receptors, such as FccRs and FceRs, are regarded as an efficient path to prevent PRRSV infection (Prather et al 2017).…”

Section: Introductionmentioning

confidence: 99%

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Porcine FcεRI Mediates Porcine Reproductive and Respiratory Syndrome Virus Multiplication and Regulates the Inflammatory Reaction

et al. 2018

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Porcine reproductive and respiratory syndrome virus (PRRSV) shows characteristic antibody-dependent enhancement (ADE) of infection and causes porcine systemic inflammation, which is similar to a type I allergic reaction; however, the role of porcine FcεRI in ADE is still unclear. In this study, the expression of different Fc receptors (FcRs) on macrophages was investigated in a PRRSV 3D4/21 cell infection model in the presence or absence of PRRSV antibody. The transcription level of FcγII and FcεRI was significantly up-regulated under PRRSV-antibody complex infection. Internalization and proliferation of PRRSV were promoted by the ADE mechanism when FcεRI was expressed in permissive 3D4/21 cells and the non-permissive cell line HEK 293T. Transcriptome sequencing data showed that the expression levels of AKT, ERK and other signal molecules in the anti-inflammatory pathway were significantly increased, especially in the cells infected with the PRRSV-antibody immune complex. Inflammatory regulatory molecules such as PLA2G6, LOX, TRPM8 and TRPM4 were significantly up-regulated following PRRSV infection but significantly down-regulated in the cells infected with the PRRSV-antibody immune complex. Our results demonstrated that FcεRI could be involved in PRRSV ADE, the antigen presenting process and regulation of the inflammatory response during PRRSV infection, which provides new insights into PRRSV infection mediated by FcεRI and the PRRSV-antibody immune complex.

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Section: Discussionmentioning

confidence: 99%

Section: Generation and Characterization Of Antibodiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Porcine FcεRI Mediates Porcine Reproductive and Respiratory Syndrome Virus Multiplication and Regulates the Inflammatory Reaction

et al. 2018

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“…FcγRIII can not only significantly inhibit the levels of IFN-α and TNF-α mRNA, but also significantly increase the levels of IL-10 and viral mRNA during PRRSV viral infection. These clarify a mechanism of PRRSV-ADE regulated by poFcγRIII, which may be to increase virus internalization in its host cells and reduce antiviral cytokine levels to promote virus entry and replication ( Gu et al, 2015 ; Zhang et al, 2016 ). FcγRIIIA promotes aggravation of dengue fever.…”

Section: The Molecular Mechanism That Produces Adementioning

confidence: 90%

Antibody dependent enhancement: Unavoidable problems in vaccine development

et al. 2021

Advances in Immunology

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“…Within PRRSV research, Yoon et al was the first to demonstrate ADE as a mechanism to increase infection with injection of sub-neutralizing amounts of IgG and identified the nucleocapsid and glycosylated envelope proteins as the targeted epitopes [ 96 ]. Over nearly three decades, additional mechanisms for PRRSV ADE have been gained to include the viral epitopes responsible: nucleocapsid protein, GP5 [ 104 ] and Fc receptors and mechanisms involved [ 105 , 106 ]. The GP5 is also identified as a viral epitope for NA [ 104 ] demonstrating the complexity in vaccine design and assessment for a virus where sub-neutralizing antibodies can increase infection.…”

Section: Plotkin’s Principles Of Correlates Of Protection Applied To ...mentioning

confidence: 99%

Testable Candidate Immune Correlates of Protection for Porcine Reproductive and Respiratory Syndrome Virus Vaccination

et al. 2023

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Porcine reproductive and respiratory syndrome virus (PRRSV) is an on-going problem for the worldwide pig industry. Commercial and experimental vaccinations often demonstrate reduced pathology and improved growth performance; however, specific immune correlates of protection (CoP) for PRRSV vaccination have not been quantified or even definitively postulated: proposing CoP for evaluation during vaccination and challenge studies will benefit our collective efforts towards achieving protective immunity. Applying the breadth of work on human diseases and CoP to PRRSV research, we advocate four hypotheses for peer review and evaluation as appropriate testable CoP: (i) effective class-switching to systemic IgG and mucosal IgA neutralizing antibodies is required for protective immunity; (ii) vaccination should induce virus-specific peripheral blood CD4+ T-cell proliferation and IFN-γ production with central memory and effector memory phenotypes; cytotoxic T-lymphocytes (CTL) proliferation and IFN-γ production with a CCR7- phenotype that should migrate to the lung; (iii) nursery, finishing, and adult pigs will have different CoP; (iv) neutralizing antibodies provide protection and are rather strain specific; T cells confer disease prevention/reduction and possess greater heterologous recognition. We believe proposing these four CoP for PRRSV can direct future vaccine design and improve vaccine candidate evaluation.

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Ligation of porcine Fc gamma receptor III inhibits levels of antiviral cytokine in response to PRRSV infection in vitro

Cited by 8 publications

References 49 publications

Porcine FcεRI Mediates Porcine Reproductive and Respiratory Syndrome Virus Multiplication and Regulates the Inflammatory Reaction

Porcine FcεRI Mediates Porcine Reproductive and Respiratory Syndrome Virus Multiplication and Regulates the Inflammatory Reaction

Antibody dependent enhancement: Unavoidable problems in vaccine development

Testable Candidate Immune Correlates of Protection for Porcine Reproductive and Respiratory Syndrome Virus Vaccination

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