Light acclimation in diatoms: From phenomenology to mechanisms

Wilhelm, Christian; Jungandreas, Anne; Jakob, Torsten; Goss, Reimund

doi:10.1016/j.margen.2013.12.003

Cited by 69 publications

(60 citation statements)

References 99 publications

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“…The consequence of this package effect is that Q a decreases with an increase of the pigment density [56]. The mean Q a phy , calculated over the whole experiment, was of 0.43 (CV = 63%, n = 37) for THAL, whereas it was of 1.09 (CV = 56%, n = 38) for CHLAM.…”

Section: Resultsmentioning

confidence: 99%

Evolution of the scattering properties of phytoplankton cells from flow cytometry measurements

et al. 2017

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After the exponential growth phase, variability in the scattering efficiency of phytoplankton cells over their complete life cycle is not well characterised. Bulk measurements are impacted by senescent cells and detritrus. Thus the analysis of the evolution of the optical properties thanks to their morphological and/or intra-cellular variations remains poorly studied. Using the Cytosense flow cytometer (CytoBuoy b.v., NL), the temporal course of the forward and sideward efficiencies of two phytoplankton species (Thalassiosira pseudonana and Chlamydomonas concordia) were analyzed during a complete life-cycle. These two species differ considerably from a morphological point of view. Over the whole experiment, the forward and sideward efficiencies of Thalassiosira pseudonana were, on average, respectively 2.2 and 1.6 times higher than the efficiencies of Chlamydomonas concordia. Large intra-species variability of the efficiencies were observed over the life cycle of the considered species. It highlights the importance of considering the optical properties of phytoplankton cells as a function of the population growth stage of the considered species. Furthermore, flow cytometry measurements were combined with radiative transfer simulations and biogeochemical and optical measurements. Results showed that the real refractive index of the chloroplast is a key parameter driving the sideward signal and that a simplistic two-layered model (cytoplasm-chloroplast) seems particularly appropriate to represent the phytoplankton cells.

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Section: Resultsmentioning

confidence: 99%

Evolution of the scattering properties of phytoplankton cells from flow cytometry measurements

et al. 2017

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“…For diatoms, low light acclimation leads to the increase in thylakoides per plastid, making the plastids optically denser to reflect light rather than absorb it, and hence lowering the quantum efficiency on a per Chl‐ a basis (Wilhelm et al. ). For T. pseudonana cultures grown under very similar conditions (Hewes ), the Chl‐ a quota (to ash free dry weight) increases from ~1% to ~3% during the phase of linear growth; the quantum efficiency per Chl‐ a may decline, but is compensated for by the increased cellular Chl‐ a concentrations.…”

Section: Resultsmentioning

confidence: 99%

The color of mass culture: spectral characteristics of a shallow water column through shade‐limited algal growth dynamics¹

Hewes

2016

Journal of Phycology

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It is envisioned that mass algal cultivation for commercial biofuels production will entail the use of large raceway pond systems, which typically have shade-limited photosynthetic growth within depths of 20-30 cm. The attenuation of light and spectral qualities of red, green, and blue wavelengths in a 20-cm water column as a function of Chl-a concentration during exponential and linear phases of growth dynamics for the marine diatom Thalassiosira pseudonana was examined under laboratory conditions. While photosynthetically available radiation (PAR) was in excess throughout the water column during the phase of exponential growth, PAR became rate limiting differently for red, green, and blue wavelengths during the phase of linear growth. The transition from exponential to linear growth occurred at 1-2 mg Chl-a · L-1, whereby a scalar ~5 μmol photons · m-2 · s-1 at 20-cm depth was found to occur as would be anticipated having the compensation point for where rates of photosynthesis and respiration are equal. During the phase of linear growth, red wavelengths became increasingly dominant at depth as Chl-a concentrations increased, being contrary to the optical conditions for those natural bodies of water that forced the evolution of phytoplankton photosynthesis. It is hypothesized this dramatic difference in water column optics between natural and synthetic environments could influence a variety of biological reactions, importantly non-photochemical quenching capacities, which could negatively impact crop yield.

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“…Light intensity and quality impact on pigment contents, photosynthesis and nutritional value of diatoms [33,34], with photosynthesis/irradiance curves commonly used to characterize photoacclimation [35–37]. Generally, benthic diatoms have a lower maximum net photosynthesis but a higher light utilization coefficient than planktonic species [10,38].…”

Section: Diatoms and The Use Of Carbon In Their Metabolismmentioning

confidence: 99%

Modulation of lipid biosynthesis by stress in diatoms

Sayanova

Mimouni

Ulmann

et al. 2017

Phil. Trans. R. Soc. B

111

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Diatoms are responsible for up to 40% of the carbon fixation in our oceans. The fixed carbon is moved through carbon metabolism towards the synthesis of organic molecules that are consumed through interlocking foodwebs, and this process is strongly impacted by the abiotic environment. However, it has become evident that diatoms can be used as ‘platform’ organisms for the production of high valuable bio-products such as lipids, pigments and carbohydrates where stress conditions can be used to direct carbon metabolism towards the commercial production of these compounds. In the first section of this review, some aspects of carbon metabolism in diatoms and how it is impacted by environmental factors are briefly described. The second section is focused on the biosynthesis of lipids and in particular omega-3 long-chain polyunsaturated fatty acids and how low temperature stress impacts on the production of these compounds. In a third section, we review the recent advances in bioengineering for lipid production. Finally, we discuss new perspectives for designing strains for the sustainable production of high-value lipids.This article is part of the themed issue ‘The peculiar carbon metabolism in diatoms’.

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Light acclimation in diatoms: From phenomenology to mechanisms

Cited by 69 publications

References 99 publications

Evolution of the scattering properties of phytoplankton cells from flow cytometry measurements

Evolution of the scattering properties of phytoplankton cells from flow cytometry measurements

The color of mass culture: spectral characteristics of a shallow water column through shade‐limited algal growth dynamics¹

Modulation of lipid biosynthesis by stress in diatoms

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Light acclimation in diatoms: From phenomenology to mechanisms

Cited by 69 publications

References 99 publications

Evolution of the scattering properties of phytoplankton cells from flow cytometry measurements

Evolution of the scattering properties of phytoplankton cells from flow cytometry measurements

The color of mass culture: spectral characteristics of a shallow water column through shade‐limited algal growth dynamics1

Modulation of lipid biosynthesis by stress in diatoms

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The color of mass culture: spectral characteristics of a shallow water column through shade‐limited algal growth dynamics¹