Light and Scanning Electron Microscopic Studies of Oviductal Epithelium in Thai Swamp Buffalo (<i>Bubalus bubalis</i>) at the Follicular and Luteal Phases

Tienthai, Paisan; Sajjarengpong, Kriengyot; Techakumphu, Mongkol

doi:10.1111/j.1439-0531.2008.01111.x

Cited by 12 publications

(10 citation statements)

References 24 publications

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“…Reproduction, a critical factor in maintaining and improving the efficacy of animal production, depends largely on the functional anatomy of female reproductive tract that plays a key role in fertilization, transportation of oocyte from ovary to uterus and development of zygote (Lauschova, Sangha et al ., ; Tienthai et al ., ; Sharma et al ., ). The oviduct, derived embryonically from the cranial regions of the primitive mullerian ducts (Price et al ., ), is divisible in four major segments – fimbriae, infundibulum, ampulla and isthmus (Hafez, ; Sharma et al ., ).…”

Section: Introductionmentioning

confidence: 99%

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Scanning and transmission electron microscopic analysis of ampullary segment of oviduct during estrous cycle in caprines

2014

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The ampullary segment of the mammalian oviduct provides suitable milieu for fertilization and development of zygote before implantation into uterus. It is, therefore, in the present study, the cyclic changes in the morphology of ampullary segment of goat oviduct were studied during follicular and luteal phases using scanning and transmission electron microscopy techniques. Topographical analysis revealed the presence of uniformly ciliated ampullary epithelia, concealing apical processes of non-ciliated cells along with bulbous secretory cells during follicular phase. The luteal phase was marked with decline in number of ciliated cells with increased occurrence of secretory cells. The ultrastructure analysis has demonstrated the presence of indented nuclear membrane, supranuclear cytoplasm, secretory granules, rough endoplasmic reticulum, large lipid droplets, apically located glycogen masses, oval shaped mitochondria in the secretory cells. The ciliated cells were characterized by the presence of elongated nuclei, abundant smooth endoplasmic reticulum, oval or spherical shaped mitochondria with crecentric cristae during follicular phase. However, in the luteal phase, secretory cells were possessing highly indented nucleus with diffused electron dense chromatin, hyaline nucleosol, increased number of lipid droplets. The ciliated cells had numerous fibrous granules and basal bodies. The parallel use of scanning and transmission electron microscopy techniques has enabled us to examine the cyclic and hormone dependent changes occurring in the topography and fine structure of epithelium of ampullary segment and its cells during different reproductive phases that will be great help in understanding major bottle neck that limits success rate in vitro fertilization and embryo transfer technology.

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Section: Introductionmentioning

confidence: 99%

“…Shirley and Reeder () described the changes in ratio of secretory and ciliated cells number in rats during estrous cycle. Similar studies on ultrastructural characteristics of oviductal epithelia were carried out on Thai swamp buffalo (Tienthai et al ., ); and Chinese meishan pig (Abe and Hoshi ).…”

Section: Introductionmentioning

confidence: 99%

Scanning and transmission electron microscopic analysis of ampullary segment of oviduct during estrous cycle in caprines

2014

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“…In the cow, for example, both ciliated and nonciliated cells are present in the UTJ with ciliation increasing during proestrus and estrus (Wrobel et al, ). In contrast, ciliation remains unchanged during the reproductive cycle in the isthmus of the Thai Swamp buffalo (Tienthai et al, ) and sheep (Yániz et al, ). The interface between the ciliated cells in the transition zone in the cow and the sparsely ciliated surface epithelium of the uterus shifts in a uterine direction during proestrus and estrus and retreats during interestrus and diestrus.…”

Section: Discussionmentioning

confidence: 99%

Morphology of the Uterotubal Junction of the Cheetah (Acinonyx jubatus)

Penfold

Soley

Hartman

2019

The Anatomical Record

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Less than 7,000 cheetahs survive in the wild. Captive breeding has proved notoriously difficult. The uterotubal junction acts as major barrier and regulator to the passage of sperm. This study describes the morphology of the uterotubal junction of the cheetah. Reproductive tracts were obtained from seven cheetahs that succumbed from natural causes or were euthanized for humane reasons. The uterotubal junction was isolated and examined macroscopically and microscopically. The extramural isthmus made a characteristic 90° bend before entering the mesometrial border of the uterus close to its tip. The intramural isthmus had approximately four primary folds lined by nonciliated cuboidal to low cuboidal epithelium. The Tunica muscularis was robust, merged with its uterine counterparts and consisted of an inner circular layer and outer longitudinal smooth muscle layer. The uterine ostium opened via a muscular and glandular papilla that projected from the apex of the uterine lumen. A sharply demarcated circular zone of simple columnar epithelial cells surrounded the uterine ostium and separated the simple cuboidal to low cuboidal cells of the isthmus and uterine epithelium from each other. Branched tubulo‐alveolar glands, some with dilated lumens, were present in the papilla and sometimes extended into the adjacent endometrium. These glands might act as sperm storage areas, and could easily be confused with cystic endometrial hyperplasia. Low transverse endometrial ridges surrounded the papilla and extended caudally for a short distance before disappearing. The uterine glands were lined by a simple cuboidal epithelium. Anat Rec, 302:1855–1864, 2019. © 2019 American Association for Anatomy

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“…Series of precisely initiated processes requiring full completion take place in the uterine tube (Besenfelder et al, 2012). Oviduct plays a key role in sperm fertilization, development of a zygote, capacitation and transport of a zygote to the uterus (Lauschova 2003, Sangha et al 2003, Tienthai et al, 2009, Sharma et al, 2013.…”

Section: Introductionmentioning

confidence: 99%

The morphological changes of oviductal mucose in oestral cycle of sows

Šťastná¹,

Šťastný²,

Miluchová³

et al. 2019

Acta fytotechn zootechn

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Licensed under a Creative Commons Attribution 4.0 International License The aim of this work was to describe microscopic and submicroscopic changes in uterine tube of 40 sows in the estral cycle. Samples of the uterine tube were obtained for histological studies by vivisection from three sections of uterine tube. Samples were fixed for light microscopy (LM) in formaldehyde and in glutaraldehyde paraformaldehyde for scanning (SEM) and transmission (TEM) electron microscopy. They were subsequently processed in the usual manner in the LM and electron microscopic studies laboratories. We did not detect progressive changes in the length of the uterine tube. Unlike the sows' weight (2.57 ±1.12 g or 2.26 ±0.96 g), the length of the uterine tube was virtually unchanged depending on the stage of the cycle (30.2 ±2.51 cm in FF or 30.1 ±2.39 cm in LF). The largest relative volume of the epithelial layer was at the follicular stage of the cycle along the entire uterine tube. The difference varied from 4.99%-isthmus to 13.62% infundibulum between each part. Significant changes were seen between the ciliary and secretory cells during the estral cycle in the various parts of the uterine tube. Ciliary cells dominated throughout the cycle in infindibulum and ampulla, whereas secretory cells in isthmus. Their changes and differentiations are the manifestations of hormonal changes that direct the estral cycle. Submicroscopic changes of cells in the estral cycle have also been described.

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Light and Scanning Electron Microscopic Studies of Oviductal Epithelium in Thai Swamp Buffalo (Bubalus bubalis) at the Follicular and Luteal Phases

Cited by 12 publications

References 24 publications

Scanning and transmission electron microscopic analysis of ampullary segment of oviduct during estrous cycle in caprines

Scanning and transmission electron microscopic analysis of ampullary segment of oviduct during estrous cycle in caprines

Morphology of the Uterotubal Junction of the Cheetah (Acinonyx jubatus)

The morphological changes of oviductal mucose in oestral cycle of sows

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