Light Dependence of Catalase Synthesis and Degradation in Leaves and the Influence of Interfering Stress Conditions

Hertwig, B; Streb, Peter; Feierabend, J.

doi:10.1104/pp.100.3.1547

Cited by 213 publications

(155 citation statements)

References 35 publications

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“…This was demonstrated in previous studies (Zhao et al 2009;Strubinskai and Hanaka 2011). The reduction in antioxidant enzyme activities under stressful conditions has been attributed to the inactivation of enzyme protein due to AOS (Verma and Dubey 2003), decrease in enzyme synthesis, or change in assembly of enzyme subunits (Hertwig et al 1992;Verma and Dubey 2003).…”

Section: Discussionsupporting

confidence: 53%

Effect of exogenous abscisic acid on the level of antioxidants in Atractylodes macrocephala Koidz under lead stress

Wang

Chen

Pan

2012

Environ Sci Pollut Res

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This study hypothesized that the positive or negative effects of exogenous abscisic acid (ABA) on oxidative stress caused by lead were dose dependent. The effects of different levels of ABA (2.5, 5, and 10 mg L −1 ) on lead toxicity in the leaves of Atractylodes macrocephala were studied by investigating plant growth, soluble sugars, proteins, lipid peroxidation, and antioxidative enzymes. Excess Pb inhibited root dry weight, root length, and the number of lateral roots, but increased shoot growth. In addition, lead stress significantly decreased the levels of chlorophyll pigments, protein, and activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and peroxidase (POD). Different levels of ABA significantly increased SOD, CAT, POD, and APX activities, but decreased the level of hydrogen peroxide and malondialdehyde in nonstressed plants. Exogenous application of 2.5 mg L −1 ABA detoxified the stress-generated damages caused by Pb and also enhanced plant growth, soluble sugars, proteins, and all four antioxidant enzyme activities but reduced Pb uptake of lead-stressed plant compared to lead treatment alone. However, the toxic effects of Pb were further increased by the applications of 5 and 10 mg L −1 ABA. The levels of antioxidants caused by a low concentration of exogenous ABA might be responsible for minimizing the Pb-induced toxicity in A. macrocephala.

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Section: Discussionsupporting

confidence: 53%

Effect of exogenous abscisic acid on the level of antioxidants in Atractylodes macrocephala Koidz under lead stress

Wang

Chen

Pan

2012

Environ Sci Pollut Res

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“…For immunoblotting, soluble supernatants and total membrane fractions of total leaf extracts were prepared as previously described (12).…”

Section: Plantmentioning

confidence: 99%

“…developed a multiplicity of antioxidative mechanisms (3,27), some components are obviously not fully protected from photodamage. Thus, the enzyme catalase is light sensitive in vitro and suffers from photoinactivation with subsequent degradation in intact leaves as well (7,12,31). To maintain a constant catalase level in light, its loss has to be continuously compensated for by an adequate concomitant resynthesis of the enzyme (12).…”

mentioning

confidence: 99%

“…Thus, the enzyme catalase is light sensitive in vitro and suffers from photoinactivation with subsequent degradation in intact leaves as well (7,12,31). To maintain a constant catalase level in light, its loss has to be continuously compensated for by an adequate concomitant resynthesis of the enzyme (12). When, however, light degradation exceeds the capacity for repair (25) or when resynthesis is impaired by inhibitors (7) or by stress conditions such as low temperature (31), an apparent loss of catalase is observed.…”

mentioning

confidence: 99%

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Photoinactivation of Catalase Occurs under Both High- and Low-Temperature Stress Conditions and Accompanies Photoinhibition of Photosystem II

Feierabend¹,

Schaan²,

Hertwig³

1992

Plant Physiol.

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179

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Severe photoinactivation of catalase (EC 1.11.1.6) and a decline of variable fluorescence (FJ), indicating photoinhibition of photosynthesis, were observed as rapid and specific symptoms in leaves exposed to a high heat-shock temperature of 40°C as well as in leaves exposed to low chilling temperatures in white light of only moderately high photosynthetic photon flux density of 520 ME m-2 s-'. Other parameters, such as peroxidase (EC 1.11.1.7), glycolate oxidase (EC 1.1.3.1), glutathione reductase (EC 1.6.4.2), or the chlorophyll content, were hardly affected under these conditions. At a compatible temperature of 22°C, the applied light intensity did not induce severe photoinactivations. In darkness, exposures to high or low temperatures did not affect catalase levels. Also, decline of F, in light was not related to temperature sensitivity in darkness. The effective low-temperature ranges inducing photoinactivation of catalase differed significantly for chilling-tolerant and chilling-sensitive plants. In leaves of rye (Secale cereale L.) and pea (Pisum sativum L.), photoinactivation occurred only below 15°C, whereas inactivation occurred at 15°C in cucumber (Cucumis sativus L.) and maize (Zea mays L.). The behavior of F, was similar, but the difference between chilling-sensitive and chillingtolerant plants was less striking. Whereas the catalase polypeptide, although photoinactivated, was not cleaved at 0 to 4°C, the Dl protein of photosystem 11 was greatly degraded during the lowtemperature treatment of rye leaves in light. Rye leaves did not exhibit symptoms of any major general photodamage, even when they were totally depleted of catalase after photoinactivation at 0 to 4°C, and catalase recovered rapidly at normal temperature. In cucumber leaves, the decline of catalase after exposures to bright light at 0 to 4°C was accompanied by bleaching of chlorophyll, and the recovery observed at 25°C was slow and required several days. Similar to the Dl protein of photosystem 11, catalase differs greatly from other proteins by its inactivation and high turnover in light. Inasmuch as catalase and Di protein levels depend on continuous repair synthesis, preferential and rapid declines are generally to be expected in light whenever translation is suppressed by stress actions, such as heat or chilling, and recovery will reflect the repair capacity of the plants.

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“…Additional stress factors, such as heat, cold, salt or toxic chemicals, may, however, either enhance the rate of catalase inactivation or inhibit protein synthesis, with the result that the loss of catalase activity exceeds the capacity for its new synthesis. Therefore, apparent photoinactivation of catalase represents an early widespread stress symptom in light, which usually accompanies photoinhibition of PSII and precedes the appearance of more general oxidative damage (Volk & Feierabend 1989;Feierabend et al 1992;Hertwig, Streb & Feierabend 1992;Streb, Michael-Knauf & Feierabend 1993;Streb & Feierabend 1996). Consequently non-adapted plants can be expected to suffer from severe declines of both catalase and PSII activity under desert conditions.…”

Section: Light Stress and Adaptationmentioning

confidence: 99%

Light stress effects and antioxidative protection in two desert plants

1997

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1. The enzyme catalase was investigated as a sensitive marker of light stress in Retama raetam stems and Atriplex halimus leaves. While the activity of catalase was readily photoinactivated in vitro when crude extracts from these tissues were exposed to a photon flux of 500μmolm–2s–1 photosynthetic active radiation (PAR), no apparent losses of the extractable catalase activities were observed when tissues were harvested from desert plants at noon when light levels were as high as 2200μmolm–2s–1 PAR. 2. When stems of R. raetam or leaves of A. halimus were exposed to natural daylight of 1000μmolm–2s–1 PAR in the presence of cycloheximide (CHI), in order to prevent the new synthesis of catalase, or at a temperature of 42–44°C, only minor losses of catalase activity occurred in stems of R. raetam, but a marked apparent photoinactivation of catalase was observed in leaves of A. halimus. 3. Similarly, stronger declines of the ratio Fv/Fm, indicating more severe photoinhibition of photosystem II (PSII), were observed in A. halimus, compared with R. raetam. 4. For R. raetam, activities of some antioxidative enzymes and the carotenoid contents were higher in plants growing in the desert than in plants collected from non‐desert locations. 5. During the daily increase of light and temperature the antioxidants ascorbate and glutathione became more oxidized and violaxanthin was converted to zeaxanthin in R. raetam stems, while these antioxidants became more reduced and zeaxanthin was not notably accumulated in A. halimus leaves. 6. The results suggest that the desert plants R. raetam and A. halimus apply different strategies of protection against light‐stress damage. In the stems of R. raetam antioxidative protection appears to play the major role, while A. halimus appears to avoid light damage in the field by the mutual shading of leaves and reflection of light at the leaf surface.

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Light Dependence of Catalase Synthesis and Degradation in Leaves and the Influence of Interfering Stress Conditions

Cited by 213 publications

References 35 publications

Effect of exogenous abscisic acid on the level of antioxidants in Atractylodes macrocephala Koidz under lead stress

Effect of exogenous abscisic acid on the level of antioxidants in Atractylodes macrocephala Koidz under lead stress

Photoinactivation of Catalase Occurs under Both High- and Low-Temperature Stress Conditions and Accompanies Photoinhibition of Photosystem II

Light stress effects and antioxidative protection in two desert plants

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