Light-driven manipulation of picobubbles on a titanium oxide phthalocyanine-based optoelectronic chip

Abstract

Microbubbles have a variety of applications in science and biological technology. Here, we demonstrate the manipulation of the picoliter gas bubble ͑picobubble͒ based on the optoelectronic-mechanism. The organic photoconductive material, titanium oxide phthalocyanine ͑TiOPc͒, was developed to make the light-sensitive substrate of this optoelectronic chip. The virtual electrodes are formed by projecting the dynamic light pattern onto TiOPc layer for generating the desired nonuniform electric field. The picobubble suspended in silicone oil can be manipulated with the velocity of 40-50 m / s. The driving force up to 160 pico-Newtons could be generated for manipulating a gas bubble of 300 picoliters.

Dielectrophoretic behavior of a single cell when manipulated by optoelectronic tweezers: A study based on COMSOL ALE simulations

“…c is the cellular velocity. Some experiments [2,4,6] can also obtain the velocity of moving cell using both forces equilibrium relation-…”

“…Some photoconductive materials in this OET operations have recently been presented. Instead of the amorphous silicon (a-Si:H), Yang et al [5,6] developed light-driven Ti-OET using an organic photoconductive material titanium oxide phthalocyanine to manipulate the HepG2 cell and picoliter gas bubble based on negative dielectrophoresis force. Moreover, lensintegrated LCD or COMS-controlled GaN LED as a micro-light source integrated into an OET device has been reported by Hwang [7] and Zarowna-Dabrowska et al [8].…”

Optoelectrokinetics-based microfluidic platform for bioapplications: A review of recent advances

“…This chip can be easily fabricated within 40 min using the spinning-coating technology. 80 It is suitable for manipulating magnetic beads, 81 picoliter gas bubbles, 82 and cell patterning 83 as well as droplets. 84 • Convenient on-chip integration with other microfluidic systems:…”

Recent Trends on Micro/Nanofluidic Single Cell Electroporation

“…The permeabilization area can be controlled with the pulse amplitude and the degree of permeabilization can be controlled with the duration of pulses, numbers of pulses, where longer pulses provide a larger perturbation area in the cell membrane [34,35]. In earlier studies of micro/nanofluidic based single cell electroporation, authors analyze cellular content and cellular properties [36][37][38][39], transfection of cells [17,[40][41][42] and inactivating cells [43][44][45] with the use of micro-channel based electroporation [46][47][48][49], micro-capillary based electroporation [50][51][52], electroporation with solid microelectrode [36,[53][54][55], membrane sandwich based microfluidic electroporation [56,57], microarray single cell electroporation [58], optofluidic based microfluidic devices [59][60][61][62][63][64][65], etc. Table 1 describes in detail micro/nanofluidic based single cell transfection, cell lysis, cell type with species, potential difference, pulse duration, etc.…”