Dielectrophoretic behavior of a single cell when manipulated by optoelectronic tweezers: A study based on COMSOL ALE simulations

Hu, Sheng; Zhao, Yong; Qian, Xiaolong

doi:10.1016/j.elstat.2015.03.004

Cited by 6 publications

(3 citation statements)

References 16 publications

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“…First, the relative permittivity of the mammalian cells was calculated using a single-shell model. The single-shell model is the simplest and most widely used model among them because the mammalian cell can usually be modeled electrically as a shelled particle [31,32]. The permittivity and conductivity of cell membrane and cytoplasm should be considered, respectively, in this model.…”

Section: Optoelectrofluidic Printing Of On-demand Patterned Hydrogel ...mentioning

confidence: 99%

“…where C m is the membrane capacitance, τ cp (=ε cp / σ cp ) and τ m (=C m •r/σ cp ) is the relaxation time of cytoplasm and media, r is the radius of cells, ε cp and σ cp are the permittivity and conductivity of cytoplasm, respectively [32]. Constants which were used in this simulation are as follows [31][32][33]: the relative permittivities of DEP buffer and PEGDA are 80 and 10, respectively [28,34]. The membrane capacitance is 0.0015 F m −2 , the radius of the liver cells is 6 μm, the relative permittivity and conductivity of the cytoplasm is 50 and 0.4 S m −1 , respectively.…”

Section: Optoelectrofluidic Printing Of On-demand Patterned Hydrogel ...mentioning

confidence: 99%

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Optoelectrofluidic printing system for fabricating hydrogel sheets with on-demand patterned cells and microparticles

Gi¹,

Han²,

Park³

2017

Biofabrication

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This paper presents a novel optoelectrofluidic printing system that facilitates not only the optoelectrofluidic patterning of microparticles and mammalian cells but also the harvesting of the patterned microparticles encapsulated within poly(ethylene glycol) dicarylate (PEGDA) hydrogel sheets. Although optoelectrofluidic technology has numerous advantages for programmable and on-demand patterning and the feasibility of manipulating single microparticles, practical applications using existing laboratory infrastructure in biological and clinical research fields have been strictly restricted due to the impossibility of recovering the final patterned product. In order to address these harvesting limitations, PEGDA was employed to utilize optoelectrofluidic printing. The Clausius-Mossotti factor was calculated to investigate the dielectrophoretic mobility of the microparticle and the cell in the PEGDA precursor solution. As a proof of concept, three basic controllabilities of the optoelectrofluidic printing system were characterized: the number of microparticles, the distance between the microparticle columns, and the ratio of two different microparticles. Furthermore, the optoelectrofluidic patterning and printing of human liver carcinoma cells (HepG2) were demonstrated in 5 min with a single-cell level of resolution. The appropriate ranges of frequency were experimentally defined based on the calculated result of the dielectrophoretic mobility of HepG2 cells. Finally, optoelectrofluidically cell-patterned hydrogel sheets were successfully recovered under a highly viable physiological condition.

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Section: Optoelectrofluidic Printing Of On-demand Patterned Hydrogel ...mentioning

confidence: 99%

Section: Optoelectrofluidic Printing Of On-demand Patterned Hydrogel ...mentioning

confidence: 99%

Optoelectrofluidic printing system for fabricating hydrogel sheets with on-demand patterned cells and microparticles

Gi¹,

Han²,

Park³

2017

Biofabrication

View full text Add to dashboard Cite

show abstract

“…For instance, a COMSOL simulation on a single HeLa (human cervical epithelioid carcinoma) cell was developed to determine electrical properties (impedance, conductivity, and permittivity) to analyze the different cellular processes using a broad selection of voltage and frequency preconditions 3 . Sheng Hu et al discussed in detail single cell dielectrophoretic (DEP) behavior in optoelectronic tweezers based on COMSOL ALE simulations 36 . Brazey et al presented a comparison of two electrode geometries, square and star shape, via FEM simulation in COMSOL and then develop a microfluidic device for experimental validation 37 .…”

Section: Introductionmentioning

confidence: 99%

Biochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel

Farooq

Butt

Hassan

2021

Sci Rep

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A biosensor capable of differentiating cells or other microparticles based on morphology finds significant biomedical applications. Examples may include morphological determination in the cellular division process, differentiation of bacterial cells, and cellular morphological variation in inflammation and cancer etc. Here, we present a novel integrated multi-planar microelectrodes geometry design that can distinguish a non-spherical individual particle flowing along a microchannel based on its electrical signature. We simulated multi-planar electrodes design in COMSOL Multiphysics and have shown that the changes in electrical field intensity corresponding to multiple particle morphologies can be distinguished. Our initial investigation has shown that top–bottom electrodes configuration produces significantly enhanced signal strength for a spherical particle as compared to co-planar configuration. Next, we integrated the co-planar and top–bottom configurations to develop a multi-planar microelectrode design capable of electrical impedance measurement at different spatial planes inside a microchannel by collecting multiple output signatures. We tested our integrated multi-planar electrode design with particles of different elliptical morphologies by gradually changing spherical particle dimensions to the non-spherical. The computed electrical signal ratio of non-spherical to spherical particle shows a very good correlation to predict the particle morphology. The biochip sensitivity is also found be independent of orientation of the particle flowing in the microchannel. Our integrated design will help develop the technology that will allow morphological analysis of various bioparticles in a microfluidic channel in the future.

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Elimination of the effect of bottom-plate capacitors in C-2C DAC using a layout technique

Khorami

Sharifkhani

2015

Microelectronics Journal

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Dielectrophoretic behavior of a single cell when manipulated by optoelectronic tweezers: A study based on COMSOL ALE simulations

Cited by 6 publications

References 16 publications

Optoelectrofluidic printing system for fabricating hydrogel sheets with on-demand patterned cells and microparticles

Optoelectrofluidic printing system for fabricating hydrogel sheets with on-demand patterned cells and microparticles

Biochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel

Elimination of the effect of bottom-plate capacitors in C-2C DAC using a layout technique

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