Light harvesting complexes of Chromera velia, photosynthetic relative of apicomplexan parasites

Tichý, Josef; Gardian, Zdenko; Bína, David; Koník, Peter; Litvín, Radek; Herbstová, Miroslava; Pain, Arnab; Vacha, František

doi:10.1016/j.bbabio.2013.02.002

Cited by 33 publications

(25 citation statements)

References 45 publications

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“…2B) and by spectroscopy (Figs S1 and S2). The CLH proteins described previously (with molecular weights in the range 19-25 kDa) [31,32] were found to be dominant in all three fractions (see asterisk in Fig. 2B).…”

Section: Isolation and Characterization Of Antenna Complexes From Chrsupporting

confidence: 64%

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Violaxanthin inhibits nonphotochemical quenching in light‐harvesting antenna of Chromera velia

et al. 2016

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Edited by Richard CogdellNon-photochemical quenching (NPQ) is a photoprotective mechanism in light-harvesting antennae. NPQ is triggered by chloroplast thylakoid lumen acidification and is accompanied by violaxanthin de-epoxidation to zeaxanthin, which further stimulates NPQ. In the present study, we show that violaxanthin can act in the opposite direction to zeaxanthin because an increase in the concentration of violaxanthin reduced NPQ in the light-harvesting antennae of Chromera velia. The correlation overlapped with a similar relationship between violaxanthin and NPQ as observed in isolated higher plant light-harvesting complex II. The data suggest that violaxanthin in C. velia can act as an inhibitor of NPQ, indicating that violaxanthin has to be removed from the vicinity of the protein to reach maximal NPQ.

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Section: Isolation and Characterization Of Antenna Complexes From Chrsupporting

confidence: 64%

“…Photosynthetic complexes separated into three main bands: top (fractions 1–3), middle (PSI, PSII supercomplexes, supercomplexes – S.C.) and low (PSI with bound antenna complexes, PSI‐LHCr); see Tichy et al . . Three upper fractions (fractions 1–3 of Fig.…”

Section: Resultsmentioning

confidence: 99%

Violaxanthin inhibits nonphotochemical quenching in light‐harvesting antenna of Chromera velia

et al. 2016

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“…A low molecular weight protein standard (Precision Plus Protein TM , Biorad, USA) was used to estimate the protein masses on the gel. MS/MS analysis was performed on a NanoAcquity UPLC (Waters, USA) online coupled to an ESI Q-TOF Premier Mass spectrometer (Waters, USA) as described in 50 .…”

Section: Methodsmentioning

confidence: 99%

Red-light phenotype in a marine diatom involves a specialized oligomeric red-shifted antenna and altered cell morphology

Herbstová

Bína

Kaňa

et al. 2017

Sci Rep

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Diatoms greatly contribute to carbon fixation and thus strongly influence the global biogeochemical balance. Capable of chromatic acclimation (CA) to unfavourable light conditions, diatoms often dominate benthic ecosystems in addition to their planktonic lifestyle. Although CA has been studied at the molecular level, our understanding of this phenomenon remains incomplete. Here we provide new data to better explain the acclimation-associated changes under red-enhanced ambient light (RL) in diatom Phaeodactylum tricornutum, known to express a red-shifted antenna complex (F710). The complex was found to be an oligomer of a single polypeptide, Lhcf15. The steady-state spectroscopic properties of the oligomer were also studied. The oligomeric assembly of the Lhcf15 subunits is required for the complex to exhibit a red-shifted absorption. The presence of the red antenna in RL culture coincides with the development of a rounded phenotype of the diatom cell. A model summarizing the modulation of the photosynthetic apparatus during the acclimation response to light of different spectral quality is proposed. Our study suggests that toggling between alternative organizations of photosynthetic apparatus and distinct cell morphologies underlies the remarkable acclimation capacity of diatoms.

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“…Transmission electron micrographs of Chromera PSI reveal a typical structure which is bound to light-harvesting complexes closely related to the PSI-antennae supercomplexes of red algae 33 , 34 . Chromera also possesses a clade of unique antennae proteins, termed “Chromera Light Harvesting” complexes that exhibit similarities to harvesting complexes from xanthophytes 33 , 34 . This is consistent with previous research indicating an ochrophyte origin of chromerid plastids 35 .…”

Section: Introductionmentioning

confidence: 99%

Extensive gain and loss of photosystem I subunits in chromerid algae, photosynthetic relatives of apicomplexans

Sobotka

Esson

Koník

et al. 2017

Sci Rep

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In oxygenic photosynthesis the initial photochemical processes are carried out by photosystem I (PSI) and II (PSII). Although subunit composition varies between cyanobacterial and plastid photosystems, the core structures of PSI and PSII are conserved throughout photosynthetic eukaryotes. So far, the photosynthetic complexes have been characterised in only a small number of organisms. We performed in silico and biochemical studies to explore the organization and evolution of the photosynthetic apparatus in the chromerids Chromera velia and Vitrella brassicaformis, autotrophic relatives of apicomplexans. We catalogued the presence and location of genes coding for conserved subunits of the photosystems as well as cytochrome b6f and ATP synthase in chromerids and other phototrophs and performed a phylogenetic analysis. We then characterised the photosynthetic complexes of Chromera and Vitrella using 2D gels combined with mass-spectrometry and further analysed the purified Chromera PSI. Our data suggest that the photosynthetic apparatus of chromerids underwent unique structural changes. Both photosystems (as well as cytochrome b6f and ATP synthase) lost several canonical subunits, while PSI gained one superoxide dismutase (Vitrella) or two superoxide dismutases and several unknown proteins (Chromera) as new regular subunits. We discuss these results in light of the extraordinarily efficient photosynthetic processes described in Chromera.

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Light harvesting complexes of Chromera velia, photosynthetic relative of apicomplexan parasites

Cited by 33 publications

References 45 publications

Violaxanthin inhibits nonphotochemical quenching in light‐harvesting antenna of Chromera velia

Violaxanthin inhibits nonphotochemical quenching in light‐harvesting antenna of Chromera velia

Red-light phenotype in a marine diatom involves a specialized oligomeric red-shifted antenna and altered cell morphology

Extensive gain and loss of photosystem I subunits in chromerid algae, photosynthetic relatives of apicomplexans

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