Violaxanthin inhibits nonphotochemical quenching in light‐harvesting antenna of <i>Chromera velia</i>

Kaňa, Radek; Kotabová, Eva; Kopečná, Jana; Trsková, Eliška Kuthanová; Belgio, Erica; Sobotka, Roman; Ruban, Alexander V.

doi:10.1002/1873-3468.12130

Cited by 28 publications

(17 citation statements)

References 59 publications

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“…Janssen and Rhiel , Kereiche et al ) than in higher plants, as previously also found for other red clade species in general (see, e.g. Büchel , Kaňa et al ). On the other hand, it may relate to the fact that chlorophyll c ‐containing antennas seem to be more delicate (see, i.e.…”

Section: Discussionsupporting

confidence: 75%

Isolation and characterization of CAC antenna proteins and photosystem I supercomplex from the cryptophytic alga Rhodomonas salina

Trsková

Bína

Santabarbara³

et al. 2019

Physiologia Plantarum

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In the present paper, we report an improved method combining sucrose density gradient with ion‐exchange chromatography for the isolation of pure chlorophyll a/c antenna proteins from the model cryptophytic alga Rhodomonas salina. Antennas were used for in vitro quenching experiments in the absence of xanthophylls, showing that protein aggregation is a plausible mechanism behind non‐photochemical quenching in R. salina. From sucrose gradient, it was also possible to purify a functional photosystem I supercomplex, which was in turn characterized by steady‐state and time‐resolved fluorescence spectroscopy. R. salina photosystem I showed a remarkably fast photochemical trapping rate, similar to what recently reported for other red clade algae such as Chromera velia and Phaeodactylum tricornutum. The method reported therefore may also be suitable for other still partially unexplored algae, such as cryptophytes.

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Section: Discussionsupporting

confidence: 75%

Isolation and characterization of CAC antenna proteins and photosystem I supercomplex from the cryptophytic alga Rhodomonas salina

Trsková

Bína

Santabarbara³

et al. 2019

Physiologia Plantarum

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“…We also observed a significant decrease in the chlorophyllto-carotenoid ratio in Rieske FeS ox plants, which would suggest that there is a greater investment in photoprotection relative to the increased chlorophyll for light harvesting. This increase in carotenoids relative to chlorophyll may contribute to the lower NPQ observed in Rieske FeS ox plants (Kaňa et al, 2016). Lutein, neoxanthin, and violaxanthin are the main xanthophyll pigment constituents of the largest lightharvesting pigment-protein complex of photosystem II (FW).…”

Section: Overexpression Of Rieske Fes Significantly Modifies the Pigmmentioning

confidence: 99%

Overexpression of the RieskeFeS Protein Increases Electron Transport Rates and Biomass Yield

et al. 2017

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“…Finally, a direct protonation of pigment-proteins has been observed in vitro (Ruban et al, 1994; Walters et al, 1996; Kaňa et al, 2012b; Xiao et al, 2012; Belgio et al, 2013). The qE sensitivity to luminal protons is controlled by various allosteric regulators (see the details of the concept in Ruban et al, 2012) including xanthophylls (e.g., zeaxanthin, and violaxanthin Niyogi et al, 1998; Kaňa et al, 2016) and the PsbS protein (see e.g., Li et al, 2000; Johnson and Ruban, 2010). …”

Section: Regulatory Role Of Protons and Ions In Triggering Non-photocmentioning

confidence: 99%

Role of Ions in the Regulation of Light-Harvesting

Kaňa

Govindjee

2016

Front. Plant Sci.

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Regulation of photosynthetic light harvesting in the thylakoids is one of the major key factors affecting the efficiency of photosynthesis. Thylakoid membrane is negatively charged and influences both the structure and the function of the primarily photosynthetic reactions through its electrical double layer (EDL). Further, there is a heterogeneous organization of soluble ions (K+, Mg2+, Cl−) attached to the thylakoid membrane that, together with fixed charges (negatively charged amino acids, lipids), provides an electrical field. The EDL is affected by the valence of the ions and interferes with the regulation of “state transitions,” protein interactions, and excitation energy “spillover” from Photosystem II to Photosystem I. These effects are reflected in changes in the intensity of chlorophyll a fluorescence, which is also a measure of photoprotective non-photochemical quenching (NPQ) of the excited state of chlorophyll a. A triggering of NPQ proceeds via lumen acidification that is coupled to the export of positive counter-ions (Mg2+, K+) to the stroma or/and negative ions (e.g., Cl−) into the lumen. The effect of protons and anions in the lumen and of the cations (Mg2+, K+) in the stroma are, thus, functionally tightly interconnected. In this review, we discuss the consequences of the model of EDL, proposed by Barber (1980b) Biochim Biophys Acta 594:253–308) in light of light-harvesting regulation. Further, we explain differences between electrostatic screening and neutralization, and we emphasize the opposite effect of monovalent (K+) and divalent (Mg2+) ions on light-harvesting and on “screening” of the negative charges on the thylakoid membrane; this effect needs to be incorporated in all future models of photosynthetic regulation by ion channels and transporters.

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Violaxanthin inhibits nonphotochemical quenching in light‐harvesting antenna of Chromera velia

Cited by 28 publications

References 59 publications

Isolation and characterization of CAC antenna proteins and photosystem I supercomplex from the cryptophytic alga Rhodomonas salina

Isolation and characterization of CAC antenna proteins and photosystem I supercomplex from the cryptophytic alga Rhodomonas salina

Overexpression of the RieskeFeS Protein Increases Electron Transport Rates and Biomass Yield

Role of Ions in the Regulation of Light-Harvesting

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