Light-induced adenovirus gene transfer, an efficient and specific gene delivery technology for cancer gene therapy

Høgset, Anders; Engesæter, Birgit; Prasmickaite, Lina; Berg, Kristian; Fodstad, Øystein; Mælandsmo, Gunhild Mari

doi:10.1038/sj.cgt.7700447

Cited by 72 publications

(59 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…24,25 However, in spite of significant increase, they were not able to transfect the entire population of cells compared to the results obtained for photochemically enhanced adenovirus-mediated gene delivery. 26 By relying on the idea that the effectiveness of nonviral transfection systems depends on the cell cycle status, 27 they investigated the role of the cell cycle status in photochemical transfection mediated by two types of synthetic transfection agents: a cationic polypeptide polylysine (polyfection) and a cationic lipid formulation N-(2-amino ethyl)-N,N-dimethyl-2,3-bis(tetradecyloxy)-1-propanaminium bromide/dioleoylphosphatidyl ethanol amine. 28 The effect of photochemical treatment on EGFP expression in cells illuminated in different cell cycle phases was compared.…”

Section: Intelligent Polymeric Vectors In Gene Therapymentioning

confidence: 99%

Intelligent polymers as nonviral vectors

2005

View full text Add to dashboard Cite

The successful gene therapy largely depends on the vector type that allows a selective and efficient gene delivery to target cells with minimal toxicity. Nonviral vectors are much safer and cheaper, can be produced easily in large quantities, and have higher genetic material carrying capacity. However, they are generally less efficient in delivering DNA and initiating gene expression as compared to viral vectors, particularly when used in vivo. As nonviral vectors, polycations may work well for efficient cell uptake and endosomal escape, because they do form compact and smaller complexes with plasmid DNA and carry amine groups, which give positive charge and buffering ability that allows safe escape from endosome/lysosome. However, this is a disadvantage in the following step, which is releasing the plasmid DNA within the cytosol. In order to initiate transcription and enhance gene expression, the polymer/plasmid complex should dissociate after releasing from endosome safely and effectively. There are also other limitations with some of the polycationic carriers, for example, aggregation, toxicity, etc. Intelligent polymers, also called as 'stimuli responsive polymers', have a great potential as nonviral vectors to obtain site-, timing-, and duration period-specific gene expression, which is already exhibited in recent studies that are briefly summarized here. Gene Therapy (2005) 12, S139-S145.

show abstract

Section: Intelligent Polymeric Vectors In Gene Therapymentioning

confidence: 99%

Intelligent polymers as nonviral vectors

2005

View full text Add to dashboard Cite

show abstract

“…20 PCI is based on disruption of the endocytic compartments in cells by light specific activation of photosensitizing compounds such as sulfonated aluminium phthalocyanines or sulfonated tetraphenyl phorphines that localize in the endocytic vesicles. 21 The mechanism and benefit of the technology has earlier been documented for nonviral vectors.…”

Section: Introductionmentioning

confidence: 99%

Transgene expression is increased by photochemically mediated transduction of polycation-complexed adenoviruses

et al. 2004

View full text Add to dashboard Cite

“…The light dose corresponding to B50% cytotoxicity was used, as PCI has been shown to be most efficient when 50% of the cells are killed. 5 The expression levels of b-galactosidase or GFP were assayed 48 hours post-infection by flow cytometry.…”

Section: Photochemical Treatmentmentioning

confidence: 99%

“…[1][2][3][4] PCI was initially documented to increase the delivery of proteins and nonviral vectors, but the technology has also shown to enhance adenoviral gene transfer by up to 20-fold. 5 Adenovirus is one of the most commonly used vectors for cancer gene therapy. However, clinical studies have revealed that limiting factors are immune response in humans and the large number of viral particles needed to obtain therapeutic effect.…”

mentioning

confidence: 99%

PCI-enhanced adenoviral transduction employs the known uptake mechanism of adenoviral particles

et al. 2005

Self Cite

View full text Add to dashboard Cite

The development of methods for efficient and specific delivery of therapeutic genes into target tissues is an important issue for further development of in vivo gene therapy. In the present study, the physical targeting technique, photochemical internalization (PCI), has been used together with adenovirus. The combination of PCI and adenoviral transduction has previously been shown to be favorable compared to adenovirus used alone, and the aim of this study was to verify the role of the adenoviral receptors and identify the uptake pathway used by adenoviral particles in photochemically treated cells. All examined cell lines showed augmented transduction efficiency after PCI-treatment, with a maximum of 13-fold increase in transgene expression compared to conventionally infected cells. Blocking of CAR induced a complete inhibition of PCI-enhanced transgene expression. However, photochemical treatment managed to enhance the transduction efficiency of the retargeted virus AdRGD-GFP showing also that the virus-CAR interaction is not vital for obtaining a photochemical effect on adenoviral transduction. Blocking the a V -integrins reduced the gene expression significantly in photochemically treated cells. Subjecting HeLa cells expressing negative mutant-dynamin to light treatment after infection gave no significant increase in gene transfer, while the gene transfer were enhanced seven-fold in cells with wild-type dynamin. Furthermore, chlorpromazine inhibited photochemical transduction in a dose-dependent manner, whereas Filipin III had no effect on the gene transfer. In summary, the data presented imply that adenoviral receptor binding is important and clathrin-mediated endocytosis is the predominant uptake mechanism for adenoviral particles in photochemically treated cells.

show abstract

Light-induced adenovirus gene transfer, an efficient and specific gene delivery technology for cancer gene therapy

Cited by 72 publications

References 24 publications

Intelligent polymers as nonviral vectors

Intelligent polymers as nonviral vectors

Transgene expression is increased by photochemically mediated transduction of polycation-complexed adenoviruses

PCI-enhanced adenoviral transduction employs the known uptake mechanism of adenoviral particles

Contact Info

Product

Resources

About