Light-induced inhibition of laccase in Pycnoporus sanguineus

Hernández, Christian; Perroni, Yareni; Pérez, José Antonio Sánchez; Rivera, Beatriz Gutiérrez; Alarcón, Enrique

doi:10.1007/s12223-015-0418-7

Cited by 9 publications

(10 citation statements)

References 24 publications

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“…This species is found in tropical and subtropical areas and is known for its high lignocellulolytic potential, for producing laccase as its main extracellular ligninase (Hernández et al, 2016), and for effectively degrading several classes of dyes (Moreira-Neto et al, 2013). However, laccase production depends on fungal cultivation and growth conditions, and the expression of different laccase isozymes is related to the presence of nutrients and inducers in the cultivation media (Valle et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Use of sugarcane molasses by Pycnoporus sanguineus for the production of laccase for dye decolorization

Marim¹,

Oliveira²,

Marquezoni³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Pycnoporus sanguineus is a white-rot basidiomycete that produces laccase as the only oxidoreductase; enzyme synthesis depends on cultivation variables, and fungal species and strain. Laccases have wide substrate specificity, oxidize a broad range of compounds, and show potential for use in dye decolorization. We evaluated laccase production in a recently isolated strain of P. sanguineus cultivated with sugarcane molasses as the only carbon source, and urea or yeast urea) increased enzymatic activity. The addition of up to 300 µM of Cu did not affect laccase production, whereas cultivation with agitation increased the activity peak by 17%. The highest laccase activity was ~50,000 U/L on the ninth day of cultivation. After 24 h, decolorization was 80% for Remazol Brilliant Blue R, 9% for Reactive Yellow 145, 6% for Reactive Red 195, and 2% for Reactive Black 5. The enzymatic extract of P. sanguineus provides a potential alternative to wastewater treatment. A better understanding of the behavior of this fungus under various culture conditions would allow improvement of the enzyme production bioprocess.

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Section: Introductionmentioning

confidence: 99%

Use of sugarcane molasses by Pycnoporus sanguineus for the production of laccase for dye decolorization

Marim¹,

Oliveira²,

Marquezoni³

et al. 2016

Genet. Mol. Res.

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“…Laccase activity by Pleurotus eringyii [39] was not significantly affected by the light conditions tested, but light stimulated its aryl-alcohol oxidase activity. On the contrary, Hernández et al [15] found out that short wavelengths (green, blue, and white) strongly inhibited laccase production by Pycnoporus sanguineus whereas maximum activity was induced in darkness. This can be explained as an ecological mechanism of environmental recognition, wood rot fungi develop in darkness inside lignocellulose structures.…”

Section: Discussionmentioning

confidence: 95%

“…For example for copper the real value is the concentration of copper in the medium and is expressed in μM. Measured responses are depicted in columns [14][15][16][17][18][19]. Values represent the mean of three replicates, SEM <5% | 953 transitions and accompanying expression of genes associated with regulation in the production of exoenzymes and with various bioprocesses in fungi such as sporulation, fruit body development, and metabolite production [37].…”

Section: Discussionmentioning

confidence: 99%

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Influence of light on lignin‐degrading activities of fungal genus Polyporus s. str

2018

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Six strains belonging to five species of Polyporus (P. arcularius, P. arcularioides, P. tricholoma, P. cfr. tricholoma, and P. varius), collected from an Atlantic Forest area in Misiones (Argentina), where species usually grow exposed to high temperatures and humidity, were identified by morphological and molecular analyses. P. tricholoma (BAFC 4536) and P. arcularioides (BAFC 4534) were selected by their lignin-degrading enzyme production, their ability to produce primordial of basidiomes under submerged fermentation, and the decrease in lignin content caused in Poplar wood (up to 29% after 45 days). Among several variables evaluated with a Plackett-Burman design (glucose, copper, vanillic acid and manganese concentration, incubation period, and light incidence), the most important factor affecting laccase and Mn-peroxidase (MnP) production by both strains, was light incidence. Light induced fruit body development but diminished laccase and MnP production. Moreover, a modified isoenzymatic laccase pattern was observed, showing additional isoenzymes when fungi were cultivated under darkness and differences in optimal temperature. Although the studied strains did not produce high laccase and MnP titers (uppermost detected 4230 and 90 U L , respectively), their laccases showed thermal stability and optimal temperature above 70 °C, representing an interesting source in the search of thermo-tolerant enzymes for biotechnological applications.

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“…Pycnoporus sanguineus (L.) Murrill (Polyporaceae) é um fungo de ocorrência tropical e subtropical comum em todo o Brasil. Considerado um FPM, apresenta elevado potencial ligninolí-tico e produz lacase como a principal enzima degradadora de lignina (HERNÁNDEZ et al, 2016).…”

Section: Introductionunclassified

PRODUÇÃO DE LACASE DE Pycnoporus sanguineus EM MEIO DE CULTIVO À BASE DE MELAÇO SOJA

Fabrini

Avelino

Marim

et al. 2017

ArqVet

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Umuarama, v. 19, n. 3, p. 159-164, jul./set. 2016. RESUMO:Lacases são polifenol oxidases que utilizam a capacidade redox de íons cobre para reduzir oxigênio a água e oxidar um substrato fenólico. A síntese e secreção de lacases de basidiomicetos dependem de vários fatores como os nutrientes presentes no meio de cultura. Visando à produção de lacase, Pycnoporus sanguineus foi cultivado em meio contendo melaço de soja como única fonte de carbono, ureia como fonte de nitrogênio suplementar em diferentes concentrações (0,6; 1,2; 2,4; 4,8 e 9,6 g/L de nitrogênio) e diferentes concentrações de CuSO 4 (0, 150, 200, 250 e 300 µM). O extrato enzimático produzido nas melhores condições de cultivo foi utilizado para a descoloração dos corantes remazol azul brilhante R (antraquinona), amarelo 145, preto 5, vermelho 195 (azo) e verde malaquita (trifenilmetano). As concentrações de nitrogênio não afetaram a produção de lacase, exceto a maior concentração (9,6 g/L) que reduziu a atividade enzimática. A adição de cobre ao meio de cultivo (150 µM) aumentou a atividade de lacase em 112%. A maior atividade de lacase (~34300 U/L) promoveu a descoloração dos corantes remazol azul brilhante R (67,5%) e verde malaquita (28,3%) em 24h, sendo os corantes azo descoloridos apenas parcialmente. Concluiu-se que o melaço de soja é um resíduo agroindustrial adequado para produção de lacase de P. sanguineus com potencial para degradação de corantes. PALAVRAS-CHAVE: Antraquinona. Corante azo. Ligninases. Poliporaceae. Resíduos agroindustriais. LACCASE PRODUCTION BY Pycnoporus sanguineus IN CULTURE MEDIA WITH SOYBEAN MOLASSESABSTRACT: Laccases are multicopper oxidases using the redox ability from copper ions to reduce oxygen to water, while oxidizing a phenolic substrate. Laccase synthesis and secretion in basidiomycetes depend on the conditions provided and on the nutrients present in the culture medium. Pycnoporus sanguineus was cultivated in medium containing soybean molasses as the sole carbon source, with urea as the source of supplemental nitrogen at different concentrations (0.6, 1.2, 2.4, 4.8 and 9.6 g/L nitrogen), and different CuSO 4 concentrations (0, 150, 200, 250 and 300 μM). The enzymatic extract produced under the best cultivation conditions was used for the depigmentation of remazole brilliant blue R (anthraquinone), yellow 145, black 5, red 195 (azo) and malachite green (triphenylmethane). Nitrogen concentrations did not affect laccase production, except for the higher concentration (9.6 g/L) reducing enzymatic activity. The addition of copper to the culture medium (150 μM) increased laccase activity by 112%. The highest laccase activity (~34300 U/L) promoted the depigmentation of remazol brilliant blue R (67.5%) and malachite green (28.3%) dyes in 24 hours. Azo dyes were only partially discolored. Therefore, it can be considered that soybean molasses is an agro-industrial byproduct suitable for the production of P. sanguineus laccase with potential for dye degradation. KEYWORDS: Agro-industrial byproduct. Anthraquinone. Azo d...

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Light-induced inhibition of laccase in Pycnoporus sanguineus

Cited by 9 publications

References 24 publications

Use of sugarcane molasses by Pycnoporus sanguineus for the production of laccase for dye decolorization

Use of sugarcane molasses by Pycnoporus sanguineus for the production of laccase for dye decolorization

Influence of light on lignin‐degrading activities of fungal genus Polyporus s. str

PRODUÇÃO DE LACASE DE Pycnoporus sanguineus EM MEIO DE CULTIVO À BASE DE MELAÇO SOJA

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