Light-induced proteolysis of myosin heavy chain by Rose Bengal-conjugated antibody complexes

Conlon, Kimberly A.; Berríos, Miguel

doi:10.1016/s1011-1344(01)00241-x

Cited by 19 publications

(16 citation statements)

References 19 publications

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“…In addition, using similar strategies, we demonstrated that nonantibody PS-conjugated protein ligands, such as rose bengal conjugated heavy meromyosin specifically photolyzed actin filaments [39]. Experiments with tissue culture cells revealed that site-directed photoproteases are as effective in vitro as in situ [37,39]. Here we describe similar in vitro results to those reported earlier using rose bengal conjugates.…”

Section: Discussionsupporting

confidence: 86%

“…We previously showed that rose bengal-conjugated primary specific antibodies or rose bengalconjugated-secondary antibodies forming a complex with untagged primary specific antibodies can induce the direct or indirect photolysis respectively of antibody-targeted proteins in the presence of visible light [37]. In addition, using similar strategies, we demonstrated that nonantibody PS-conjugated protein ligands, such as rose bengal conjugated heavy meromyosin specifically photolyzed actin filaments [39].…”

Section: Discussionmentioning

confidence: 96%

“…ester of rose bengalhexanoic acid (RBHA) to rabbit anti-mOGG1 antibodies and affinity purified goat anti-rabbit IgG antibodies-The synthesis of the NHS ester of RBHA was previously described [37]. Rabbit anti-mOGG1 antibodies and affinity purified goat anti-rabbit IgG antibodies were labeled with rose bengal as described previously [38].…”

Section: Conjugation Of the N-hydroxysuccinimide (Nhs)mentioning

confidence: 99%

“…All mixtures were incubated at room temperature in the dark for 15 min. Reaction mixtures were irradiated for 30 min at 25°C with visible light from an illumination head connected through an 80 cm long, 7 mm diameter fiber optic to a fan cooled 150 W halogen lamp as previously described [37]. The lamp spectral data is available at www.schott-fostec.com.…”

Section: 27mentioning

confidence: 99%

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Site-directed photoproteolysis of 8-oxoguanine DNA glycosylase 1 (OGG1) by specific porphyrin-protein probe conjugates: A strategy to improve the effectiveness of photodynamic therapy for cancer

Conlon

Berríos

2007

Journal of Photochemistry and Photobiology B: Biology

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The specific light-induced, non-enzymatic photolysis of mOGG1 by porphyrin-conjugated or rose bengal-conjugated streptavidin and porphyrin-conjugated or rose bengal-conjugated first specific or secondary anti-IgG antibodies is reported. The porphyrin chlorin e6 and rose bengal were conjugated to either streptavidin, rabbit anti-mOGG1 primary specific antibody fractions or goat anti-rabbit IgG secondary antibody fractions. Under our experimental conditions, visible light of wavelengths greater than 600 nm induced the non-enzymatic degradation of mOGG1 when this DNA repair enzyme either directly formed a complex with chlorin e6-conjugated anti-mOGG1 primary specific antibodies or indirectly formed complexes with either streptavidin-chlorin e6 conjugates and biotinylated first specific anti-mOGG1 antibodies or first specific anti-mOGG1antibodies and chlorin e6-conjugated anti-rabbit IgG secondary antibodies. Similar results were obtained when rose bengal was used as photosensitizer instead of chlorine e6. The rate of the photochemical reaction of mOGG1 site-directed by all three chlorine e6 antibody complexes was not affected by the presence of the singlet oxygen scavenger sodium azide. Site-directed photoactivatable probes having the capacity to generate reactive oxygen species (ROS) while destroying the DNA repair system in malignant cells and tumors may represent a powerful strategy to boost selectivity, penetration and efficacy of current photodynamic (PDT) therapy methodologies.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 96%

Section: Conjugation Of the N-hydroxysuccinimide (Nhs)mentioning

confidence: 99%

Section: 27mentioning

confidence: 99%

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Site-directed photoproteolysis of 8-oxoguanine DNA glycosylase 1 (OGG1) by specific porphyrin-protein probe conjugates: A strategy to improve the effectiveness of photodynamic therapy for cancer

Conlon

Berríos

2007

Journal of Photochemistry and Photobiology B: Biology

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“…Erythrosine belongs to a class of cyclic compounds called xanthenes, which absorb light in the visible region, and the ability of erythrosine to initiate photochemical reactions is well documented 12,13) . Erythrosine has an advantage over other photosensitizers, as it already targets dental plaque and has full approval for use in the mouth 14) .…”

Section: ⅰ Introductionmentioning

confidence: 99%

The Effect of Photodynamic Therapy on the Viability of Streptococcus Mutans Isolated From Oral Cavity

Jung¹,

Park²,

Lee

et al. 2012

THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY

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Photodynamic therapy (PDT) is a technique that involves the activation of photosensitizer by light in the presence of tissue oxygen, resulting in the production of reactive radicals capable of inducing cell death. The aim of this study was to evaluate the effect of PDT on Streptococcus mutans in planktonic conditions, previously treated with different photosensitive concentrations of erythrosine, using halogen and LED curing unit as a light source. And we compared the effects of PDT on six strains of S. mutans isolated from oral cavity and reference strain.As a result, S. mutans was susceptible to the combination of hand held photopolymerizer (HHP) and erythrosine. The higher concentration of erythrosine in the presence of light irradiation induced greater effects in reduction of viability of S. mutans. Isolated S. mutans showed a significant reduction in bacterial counts of the groups submitted to PDT compared to the control groups. And they appeared to be similar or slightly lower antimicrobial effect compared with reference strain. However, the difference was not significant (p < 0.05).In conclusion, PDT using erythrosine as a photosensitizing agent and HHP as a light source could be an efficient option for diseases caused by S. mutans.

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Aggregation‐Induced Emission Gold Clustoluminogens for Enhanced Low‐Dose X‐ray‐Induced Photodynamic Therapy

et al. 2019

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The use of gold nanoparticles as radiosensitizers is an effective way to boost the killing efficacy of radiotherapy while drastically limiting the received dose and reducing the possible damage to normal tissues. Herein, we designed aggregation‐induced emission gold clustoluminogens (AIE‐Au) to achieve efficient low‐dose X‐ray‐induced photodynamic therapy (X‐PDT) with negligible side effects. The aggregates of glutathione‐protected gold clusters (GCs) assembled through a cationic polymer enhanced the X‐ray‐excited luminescence by 5.2‐fold. Under low‐dose X‐ray irradiation, AIE‐Au strongly absorbed X‐rays and efficiently generated hydroxyl radicals, which enhanced the radiotherapy effect. Additionally, X‐ray‐induced luminescence excited the conjugated photosensitizers, resulting in a PDT effect. The in vitro and in vivo experiments demonstrated that AIE‐Au effectively triggered the generation of reactive oxygen species with an order‐of‐magnitude reduction in the X‐ray dose, enabling highly effective cancer treatment.

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Light-induced proteolysis of myosin heavy chain by Rose Bengal-conjugated antibody complexes

Cited by 19 publications

References 19 publications

Site-directed photoproteolysis of 8-oxoguanine DNA glycosylase 1 (OGG1) by specific porphyrin-protein probe conjugates: A strategy to improve the effectiveness of photodynamic therapy for cancer

Site-directed photoproteolysis of 8-oxoguanine DNA glycosylase 1 (OGG1) by specific porphyrin-protein probe conjugates: A strategy to improve the effectiveness of photodynamic therapy for cancer

The Effect of Photodynamic Therapy on the Viability of Streptococcus Mutans Isolated From Oral Cavity

Aggregation‐Induced Emission Gold Clustoluminogens for Enhanced Low‐Dose X‐ray‐Induced Photodynamic Therapy

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