Light Inhibition of Shoot Regeneration Is Regulated by Endogenous Abscisic Acid Level in Calli Derived from Immature Barley Embryos

Rikiishi, Kazuhide; Matsuura, Takakazu; Ikeda, Yoko; Maekawa, Masahiko

doi:10.1371/journal.pone.0145242

Cited by 9 publications

(4 citation statements)

References 67 publications

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“…However, some studies, including our previous works, found that ABA was highly accumulated in HRC, but not in NRC [5]. Furthermore, the expression profiles of ABA biosynthesis genes were also upregulated, which match with our results [48].…”

Section: Roles Of Aba On Regenerable Callus Inductionsupporting

confidence: 91%

Cross Talk among Phytohormone Signal and Carbohydrate Metabolism Involving Regenerable Calli Induction under Osmotic Treatment

Lee¹,

Huang²

2020

Plant Science - Structure, Anatomy and Physiology in Plants Cultured in Vivo and in Vitro

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Nonregenerable calli (NRC) derived from immature seeds of japonica rice were inoculated on MS medium containing 10 μM 2,4-D (MSD10). They turned to highly regenerable calli (HRC) when sorbitol was supplemented into the medium. Meanwhile, high levels of endogenous IAA and ABA were accumulated in HRC. Exogenous IAA precursor and ABA in MSD10 have the same effect to enhance regeneration ability. However, there are only partial effects if IAA precursor or ABA was supplemented, respectively. The regeneration ability is prominently decreased from 75% to 25% while an auxin transport inhibitor, 2,3,5-triiodobenzoic acid, was included in the medium. It suggested that endogenous auxin signal and ABA may involve in the induction of HRC. Furthermore, it showed higher contents of glucose, sucrose, and starch and higher expression levels of wall-bound invertase 1, sucrose transporter 1 (OsSUT1), and OsSUT2 genes in HRC than in NRC. The expression levels of PIN-formed 1 and LEA1 were also consistent with the trend of carbohydrate metabolisms. We thus concluded a flowchart for HRC induction by osmotic stress. According to the hypothesis, osmotic stress may regulate endogenous levels of auxin interacting with ABA, then affect carbohydrate metabolism to trigger callus initiation and further shoot regeneration in rice.

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Section: Roles Of Aba On Regenerable Callus Inductionsupporting

confidence: 91%

Cross Talk among Phytohormone Signal and Carbohydrate Metabolism Involving Regenerable Calli Induction under Osmotic Treatment

Lee¹,

Huang²

2020

Plant Science - Structure, Anatomy and Physiology in Plants Cultured in Vivo and in Vitro

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“…GA regulates plant growth by influencing stem growth and inducing germination [56], and the receptor protein GID1, DELLA protein, and PIF are involved in the signal transduction pathway of GA. In the tissue culture of immature barley embryo, the accumulation of ABA inhibited the shoot regeneration of the callus derived from immature barley embryos [57]. The downregulated DEGs related to the transduction of GA and ABA in our study might play a role in the regeneration of EC derived from immature maize embryos.…”

Section: Discussionmentioning

confidence: 60%

Transcriptome sequencing analysis of maize embryonic callus during early redifferentiation

et al. 2019

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Background Maize is one of the primary crops of genetic manipulation, which provides an excellent means of promoting stress resistance and increasing yield. However, the differences in induction and regeneration capacity of embryonic callus (EC) among various genotypes result in genotypic dependence in genetic transformation. Results In this study, embryonic calli of two maize inbred lines with strong redifferentiation capacity and two lines with weak redifferentiation capability were separately subjected to transcriptome sequencing analysis during the early redifferentiation stages (stage I, 1–3 d; stage II, 4–6 d; stage III, 7–9 d) along with their corresponding controls. A total of ~ 654.72 million cDNA clean reads were yielded, and 62.64%~ 69.21% clean reads were mapped to the reference genome for each library. In comparison with the control, the numbers of differentially expressed genes (DEGs) for the four inbred lines identified in the three stages ranged from 1694 to 7193. By analyzing the common and specific DEGs of the four materials, we found that there were 321 upregulated genes and 386 downregulated genes identified in the high-regeneration lines (141 and DH40), whereas 611 upregulated genes and 500 downregulated genes were specifically expressed in the low-regeneration lines (ZYDH381–1 and DH3732). Analysis of the DEG expression patterns indicated a sharp change at stage I in both the high- and low-regeneration lines, which suggested that stage I constitutes a crucial period for EC regeneration. Notably, the specific common DEGs of 141 and DH40 were mainly associated with photosynthesis, porphyrin and chlorophyll metabolism, ribosomes, and plant hormone signal transduction. In contrast, the DEGs in ZYDH381–1 and DH3732 were mainly related to taurine and hypotaurine metabolism, nitrogen metabolism, fatty acid elongation, starch and sucrose metabolism, phenylpropanoid biosynthesis, and plant circadian rhythm. More importantly, WOX genes, which have an ancestral role in embryo development in seed plants and promote the regeneration of transformed calli, were specifically upregulated in the two high-regeneration lines. Conclusions Our research contributes to the elucidation of molecular regulation during early redifferentiation in the maize embryonic callus. Electronic supplementary material The online version of this article (10.1186/s12864-019-5506-7) contains supplementary material, which is available to authorized users.

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“…However, they found that JA was involved in the ozone response instead of IAA. Moreover, their working hypothesis was that differences in the contents of auxin and cytokinins in the calli of barley explain the difference in regeneration efficiency among cultivars in the course of experiments reported by Hisano et al (2016) and Rikiishi et al (2015). However, these two independent studies demonstrated that regeneration capacity could be explained by differences in ABA content.…”

Section: Simultaneous Analysis Of Multiple Phytohormonesmentioning

confidence: 99%

Phytohormones in red seaweeds: a technical review of methods for analysis and a consideration of genomic data

Mori¹,

Ikeda²,

Matsuura

et al. 2017

Botanica Marina

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Emerging studies suggest that seaweeds contain phytohormones; however, their chemical entities, biosynthetic pathways, signal transduction mechanisms, and physiological roles are poorly understood. Until recently, it was difficult to conduct comprehensive analysis of phytohormones in seaweeds because of the interfering effects of cellular constituents on fine quantification. In this review, we discuss the details of the latest method allowing simultaneous profiling of multiple phytohormones in red seaweeds, while avoiding the effects of cellular factors. Recent studies have confirmed the presence of indole-3-acetic acid (IAA), N 6 -(Δ2-isopentenyl)adenine (iP), (+)-abscisic acid (ABA), and salicylic acid, but not of gibberellins and jasmonate, in Pyropia yezoensis and Bangia fuscopurpurea. In addition, an in silico genome-wide homology search indicated that red seaweeds synthesize iP and ABA via pathways similar to those in terrestrial plants, although genes homologous to those involved in IAA biosynthesis in terrestrial plants were not found, suggesting the epiphytic origin of IAA. It is noteworthy that these seaweeds also lack homologues of known factors involved in the perception and signal transduction of IAA, iP, and ABA. Thus, the modes of action of these phytohormones in red seaweeds are unexpectedly dissimilar to those in terrestrial plants.

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Light Inhibition of Shoot Regeneration Is Regulated by Endogenous Abscisic Acid Level in Calli Derived from Immature Barley Embryos

Cited by 9 publications

References 67 publications

Cross Talk among Phytohormone Signal and Carbohydrate Metabolism Involving Regenerable Calli Induction under Osmotic Treatment

Cross Talk among Phytohormone Signal and Carbohydrate Metabolism Involving Regenerable Calli Induction under Osmotic Treatment

Transcriptome sequencing analysis of maize embryonic callus during early redifferentiation

Phytohormones in red seaweeds: a technical review of methods for analysis and a consideration of genomic data

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