Light inhibits spore germination through phytochrome in Aspergillus nidulans

Röhrig, Julian; Kästner, Christian; Fischer, Reinhard

doi:10.1007/s00294-013-0387-9

Cited by 60 publications

(44 citation statements)

References 36 publications

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“…Dixon agar medium was prepared as previously described [4]. Following previously published protocols [3], [19], [20] the agar plates were kept in plastic bags with humid gas to prevent desiccation and incubated aerobically at room temperature (∼25°C) in the dark [21]. Dixon agar medium plates were incubated aerobically at 30°C.…”

Section: Methodsmentioning

confidence: 99%

Eukaryote Culturomics of the Gut Reveals New Species

2014

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The repertoire of microeukaryotes in the human gut has been poorly explored, mainly in individuals living in northern hemisphere countries. We further explored this repertoire using PCR-sequencing and culture in seven individuals living in four tropical countries. A total of 41 microeukaryotes including 38 different fungal species and three protists were detected. Four fungal species, Davidiella tassiana, Davidiella sp., Corticiaceae sp., and Penicillium sp., were uniquely detected by culture; 27 fungal species were uniquely detected using PCR-sequencing and Candida albicans, Candida glabrata, Trichosporon asahii, Clavispora lusitaniae, Debaryomyces hansenii, Malassezia restricta, and Malassezia sp. were detected using both molecular and culture methods. Fourteen microeukaryotes were shared by the seven individuals, whereas 27 species were found in only one individual, including 11 species in Amazonia, nine species in Polynesia, five species in India, and two species in Senegal. These data support a worldwide distribution of Malassezia sp., Trichosporon sp., and Candida sp. in the gut mycobiome. Here, 13 fungal species and two protists, Stentor roeseli and Vorticella campanula, were observed for first time in the human gut. This study revealed a previously unsuspected diversity in the repertoire of human gut microeukaryotes, suggesting spots for further exploring this repertoire.

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Section: Methodsmentioning

confidence: 99%

Eukaryote Culturomics of the Gut Reveals New Species

2014

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“…Blue and red light also act cooperatively/additively to repress two processes: the first is sexual development in the form of cleistothecium formation (Purschwitz et al , 2008); the second is conidial germination (Röhrig et al , 2013). In both cases the repression may serve as a possible protective mechanism, ensuring that sex or growth are not initiated in the presence of genotoxic and deleterious ultra-violet radiation (Fuller et al , 2014).…”

Section: Aspergillus Nidulans: a Model For Photosensory Cross-talkmentioning

confidence: 99%

“…This supports a model in which FphA serves as an activator of germination in the dark and this activity is repressed by light. Not only do the WCC and cryA mutants not display a germination defect, they still exhibit germination suppression by blue light (Röhrig et al , 2013). This indicates that an additional, yet to be identified, blue light receptor senses blue light and impinges upon FphA.…”

Section: Aspergillus Nidulans: a Model For Photosensory Cross-talkmentioning

confidence: 99%

Seeing the world differently: variability in the photosensory mechanisms of two model fungi

Dasgupta

Fuller

Dunlap

et al. 2015

Environmental Microbiology

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Summary Light plays an important role for most organisms on this planet, serving either as a source of energy or information for the adaptation of biological processes to specific times of day. The fungal kingdom is estimated to contain well over a million species, possibly ten-fold more, and it is estimated that a majority of the fungi respond to light, eliciting changes in several physiological characteristics including pathogenesis, development and secondary metabolism. Two model organisms for photobiological studies have taken center-stage over the last few decades – Neurospora crassa and Aspergillus nidulans. In this review, we will first discuss our understanding of the light response in N. crassa, about which the most is known, and will then juxtapose N. crassa with A. nidulans which, as will be described below, provides an excellent template for understanding photosensory cross-talk. Finally, we will end with a commentary on the variability of the light response among other relevant fungi, and how our molecular understanding in the aforementioned model organisms still provides a strong base for dissecting light responses in such species.

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“…In Aspergillus fungi, this wavelength (red to distant red) is detected by FphA phytochromes, which intervene in physiological processes such as inhibition of spore germination (Röhrig et al 2013) and the repression of sexual development (Blumenstein et al 2005) in Aspergillus nidulans. However, red light has not been associated to the induction/inhibition of enzymes involved in degradation of lignocellulose.…”

Section: Discussionmentioning

confidence: 99%

Light-induced inhibition of laccase in Pycnoporus sanguineus

et al. 2015

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The aim was to determine which specific regions of the visible light spectrum were responsible for the induction or inhibition of laccase in Pycnoporus sanguineus. Cultures were exposed to various bandwidth lights: blue (460 nm), green (525 nm), white (a combination of 460 and 560 nm), red (660 nm), and darkness. The results indicate that short wavelengths strongly inhibit the production of laccase: green (3.76 ± 1.12 U/L), blue (1.94 ± 0.36 U/L), and white (1.05 ± 0.21 U/L) in proportions of 85.8, 92.6, and 96.0%, respectively; whereas long wavelengths inhibit laccase production only partially i.e., red light (14.05 ± 4.79 U/L) in a proportion of 46.8%. Maximum activity was induced in absence of visible light (30 °C, darkness), i.e., 30.76 ± 4.0 U/L. It is concluded that the production of laccase in P. sanguineus responds to light stimuli [measured as wavelengths and lx] and that it does so inversely. This can be explained as an ecological mechanism of environmental recognition, given that P. sanguineus develops inside lignocellulose structures in conditions of darkness. The presence of short wavelength light (460-510 nm) would indicate that the organism finds itself in an external environment, unprovided of lignin, and that it is therefore unnecessary to secrete laccase. This possible new regulation in the laccase production in P. sanguineus has important biotechnological implications, for it would be possible to control the production of laccase using light stimuli.

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Light inhibits spore germination through phytochrome in Aspergillus nidulans

Cited by 60 publications

References 36 publications

Eukaryote Culturomics of the Gut Reveals New Species

Eukaryote Culturomics of the Gut Reveals New Species

Seeing the world differently: variability in the photosensory mechanisms of two model fungi

Light-induced inhibition of laccase in Pycnoporus sanguineus

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