Light regulation of asexual development in the rice blast fungus, Magnaporthe oryzae

Lee, Kwangwon; Singh, Pratibha; Chung, W. C.; Ash, Joshua S.; Kim, Tae Sung; Hang, Lisa E.; Park, Sohyun

doi:10.1016/j.fgb.2006.04.005

Cited by 110 publications

(95 citation statements)

References 70 publications

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“…The WC-light sensing system has been studied in a number of fungi, such as the ascomycetes Trichoderma (Gressel and Galun, 1967), Magnaporthe grisea (now called M. oryzae) (Lee et al, 2006) and Bipolaris oryzae (Kihara et al, 2004). The discovery of these proteins in ascomycetes, basidiomycetes like Cryptococcus neoformans, and in zygomycetes like Phycomyces blakesleeanus and their corresponding functions as light sensors clearly suggests that the WCC arose early in fungal evolution as a photoreceptor and a transcription factor to regulate fungal photoresponses (Corrochano, 2007;Herrera-Estrella and Horwitz, 2007;Purschwitz et al, 2006Purschwitz et al, , 2008Sanz et al, 2009).…”

Section: Blue-light Sensing -The White Collar Systemmentioning

confidence: 99%

Spotlight on Aspergillus nidulans photosensory systems

Bayram

Braus

Fischer

et al. 2010

Fungal Genetics and Biology

156

148

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Section: Blue-light Sensing -The White Collar Systemmentioning

confidence: 99%

Spotlight on Aspergillus nidulans photosensory systems

Bayram

Braus

Fischer

et al. 2010

Fungal Genetics and Biology

156

148

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“…1A), which imparts a fluffy appearance to the colony. 3 As depicted in Figure 1A, the conidiophore, or conidiogenous hypha, is composed of a stalk and distinguished through a bulbous swelling at its tip. The bulbous outgrowth finally develops into a spindle-shaped conidium, and eventually a septum delineates the conidium from the stalk.…”

Section: Introductionmentioning

confidence: 99%

“…Rather surprisingly, very little is known about the genetic control of conidia formation in Magnaporthe. [3][4][5][6][7][8] Macroautophagy (hereafter autophagy), a highly conserved catabolic process in eukaryotes, is responsible for organellar turnover, membrane recycling and lysosomal (vacuolar) degradation of proteins. Autophagy is induced during several biological processes, including response to environmental stress or pathogen invasion, and cellular remodeling during development and differentiation.…”

Section: Introductionmentioning

confidence: 99%

Autophagy-assisted glycogen catabolism regulates asexual differentiation inMagnaporthe oryzae

Deng¹,

Ramos‐Pamplona²,

Naqvi³

2009

Autophagy

138

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Autophagy, a conserved pathway for bulk cellular degradation and recycling in eukaryotes, regulates proper turnover of organelles, membranes and certain proteins. Such regulated degradation is important for cell growth and development, particularly during environmental stress conditions, which act as key inducers of autophagy. We found that autophagy and MoATG8 were significantly induced during asexual development in Magnaporthe oryzae. An RFP-tagged MoAtg8 showed specific localization and enrichment in aerial hyphae, conidiophores and conidia. We confirmed that loss of MoATG8 results in dramatically reduced ability to form conidia, the asexual spores that propagate riceblast disease. Exogenous supply of glucose or sucrose significantly suppressed the conidiation defects in a MoATG8-deletion mutant. Comparative proteomics-based identification and characterization of Gph1, a glycogen phosphorylase that catalyzes glycogen breakdown, indicated that autophagy-assisted glycogen homeostasis is likely important for proper aerial growth and conidiation in Magnaporthe. Loss of Gph1, or addition of G6P, significantly restored conidiation in the Moatg8Δ mutant. Overproduction of Gph1 led to reduced conidiation in wild-type Magnaporthe strain. We propose that glycogen autophagy actively responds to and regulates carbon utilization required for cell growth and differentiation during asexual development in Magnaporthe.

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“…The conidial formation in the rice blast fungus is a complicated process, affected by many known signal pathways, such as cyclic adenosine monophosphate (cAMP) signaling (Adachi and Hamer, 1998), mitogenactivated protein (MAP) kinase (Zhao et al, 2007), and autophagy pathways (Veneault-Fourrey et al, 2006;Liu et al, 2007). The conidiation was also affected by deletion of the following genes such as funga1 hydrophobin gene MHP1 (Kim et al, 2005), PAK kinase genes CHM1 and MST20 (Li et al, 2004), G-beta subunit gene MGB1 (Nishimura et al, 2003), a blue light receptor gene MgWC-1 (Lee et al, 2006), and a type III integral transmembrane protein encoding gene MTP1 . Although the mechanism of conidiation reduction in ∆moflp1 mutants remains unclear, it was reported that conidium formation relies on the endogenous sources for nutrient supply through autophagy in Aspergillus oryzae or M. oryzae (Kikuma et al, 2006;Liu et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

MoFLP1, encoding a novel fungal fasciclin-like protein, is involved in conidiation and pathogenicity in Magnaporthe oryzae

Liu

Chen

Min

et al. 2009

J. Zhejiang Univ. Sci. B

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Fasciclin family proteins have been identified as cell adhesion molecules in various organisms. In this study, a novel Magnaporthe oryzae fasciclin-like protein encoding gene, named MoFLP1, was isolated from a subtractive suppressive cDNA library and functionally analyzed. Sequence analysis showed that the MoFLP1 gene contains an open reading frame (ORF) of 1050 nucleotides encoding 349 amino acids with a calculated molecular weight of 35.85 kDa and a pI of 7.76. The deduced MoFLP1 protein contains a 17-amino acid secretion signal sequence and an 18-amino acid sequence with the characteristics of a glycosylphosphotidylinositol (GPI) anchor additional signal at its N-and C-terminuses, respectively. Potential N-glycosylation sites and domains involving cell adhesion were also identified in MoFLP1. Sequence analysis and subcellular localization by the expression of MoFLP1-GFP fusion construct in M. oryzae indicated that the MoFLP1 protein is probably localized on the vacuole membrane. Two MoFLP1 null mutants generated by targeted gene disruption exhibited marked reduction of conidiation, conidial adhesion, appressorium turgor, and pathogenicity. Our results indicate that fasciclin proteins play important roles in fungal development and pathogenicity in M. oryzae.

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Light regulation of asexual development in the rice blast fungus, Magnaporthe oryzae

Cited by 110 publications

References 70 publications

Spotlight on Aspergillus nidulans photosensory systems

Spotlight on Aspergillus nidulans photosensory systems

Autophagy-assisted glycogen catabolism regulates asexual differentiation inMagnaporthe oryzae

MoFLP1, encoding a novel fungal fasciclin-like protein, is involved in conidiation and pathogenicity in Magnaporthe oryzae

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