Light scattering application for bacterial cell monitoring during cultivation process

Kotsyumbas, Ihor; Kushnir, I. M.; Bilyy, Rostyslav; Yarynovska, Ivanna H.; Getman, Vasyl B.; Bilyi, Alexander I.

doi:10.1364/ecbo.2007.6631_57

Cited by 4 publications

(4 citation statements)

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“…Those cells were chosen as a testing model because their integrity can be easily evaluated by conventional methods (microscopy, agglutination observation), they were proven to be a good model for study the interaction with nanoparticles of different nature [9], besied the tests can be scaled up for automated analysis as described previously [10,11]. Human leukemia Jurkat T-cells were cultured in RPMI 1640 culture medium supplemented with 10% of heat inactivated fetal calf serum (Sigma) and gentamycin (50 mg/ml, Sigma).…”

Section: Methodsmentioning

confidence: 99%

The interaction of the carbon nanoparticles with human cell plasma membrane

et al. 2013

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The study of carbon nanostructures is a highly topical branch of bionanotechnology because of their potential application in biomedicine. Carbon nanotubes (CNTs) are known for their ability to kill tumor cells causing hyperthermia shock and can be used in photothermal therapy respectively. Also chemically modified CNTs can be used for drug delivery. The needle-like shape of CNTs allows them to penetrate into the cell plasma membrane without killing the cell. C 60 fullerenes are regarded as valuable nanocarriers for different hydrophobic molecules as well as potential antiviral agents or photosensitizers.In our previous studies we have demonstrated that all types of carbon nanoparticles cause externalization of phosphatidylserine (PS) from the inner to the outer layer of the cell membrane in the small local patches (points of contact), leaving the other parts of plasma membrane PS-negative. In the current work there were studied the interactions of pristine C 60 fullerenes and different types of CNTs with human blood cells (erythrocytes and Jurkat T-cells). We have shown, that carbon nanoparticles do not have any hemolytic effects, if judged by the dynamics of acidic hemolysis, although they are capable of permeabilizating the cells and facilitating the internalization of propidium iodide into the nuclei.

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Section: Methodsmentioning

confidence: 99%

The interaction of the carbon nanoparticles with human cell plasma membrane

et al. 2013

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“…Matrix determination of this system and vector of measured dependence of impulses number from duration and amplitude is conducted according to the next algorithm. Consequently, within the framework of taken model, the problem of size distribution determination is reduced to finding vector x by solving system of linear algebra equations which can be written down as matrix: b Ax = (2) b -is and vector of results of measuring the components of which determine from the matrix of distributing of dissipated of light scattering impulse, registered after their amplitude and duration, A -of matrix the elements of which determine at and graduation.…”

Section: Particles' Size Distribution Methodsmentioning

confidence: 99%

A new device for registration of bacterial cells

et al. 2011

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Device for registration of bacterial cells it is possible to examine as an information task which is characterized by a priori vagueness of moment of appearance of prototype system in the area of their registration. These reasons do not allow to provide exactness measuring on the base of measuring charts, functioning of which is provided structurally, and require the program hardware methods of measuring. An effective decision of this task is possible subject to condition application of the adaptive, program-driven methods, realized on the base of the fast-acting specialized charts of high integration. A new device for registration of bacterial cells in liquid medium after the changes of them size distributing is described. Procedure of registration of bacterial cells consists in dissociating of signal from noises, amplitude and duration measurement of the proper electric impulses. The process of registration particles in real-time imaging the screening on monitor of the personal computer. The developed software allows estimating the size distributing of the explored objects and their concentration in a liquid medium in 3D-imaging. There possibilities of device from registration and visualization of bacterial cells of different nature are presented.

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“…By authors first it is offered for determination of quantitative changes of cells of bacteria in the process of their cultivation to use the morphological changes of cells by determination of the size distributing of cells of bacteria in a liquid vivifying medium after the changes by them of intensity light scattering [23]. The process of cultivation of bacterial cells in the growth media is accompanied by the changes their size distribution.…”

Section: Bacterial Cells Monitoring By Light Scattering Methodmentioning

confidence: 99%

Rapid detection of bacterial cells by light scattering method

et al. 2008

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Method of rapid detection of bacterial cells by light scattering is described. Determination of quantitative changes of bacteria is the given method based on the changes of their size distributing in the process of cultivation. Liquid medial are diluted and analyzed by the proposed technology to determine presence of bacteria. A method includes sounding of flow suspended bacterial cells by monochromatic coherent light, registration of signals of co-operation of sounding radiation with the explored microbiological objects by detects amplitudes and durations of scattered light impulses. Distribution of particles by sizes is determined from the measured functional dependence of number of registered particles from amplitude and duration of the proper electric impulses on the output photoreceiver. Detection is done for a range of particle size from 0.1 to 10 mkm, and thus particle's size distribution is determined. The results of studying of rapid detection of Escherichia coli by light scattering are described.

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Light scattering application for bacterial cell monitoring during cultivation process

Cited by 4 publications

References 0 publications

The interaction of the carbon nanoparticles with human cell plasma membrane

The interaction of the carbon nanoparticles with human cell plasma membrane

A new device for registration of bacterial cells

Rapid detection of bacterial cells by light scattering method

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