Light scattering, chlorophyll fluorescence and state of the adenylate system in illuminated spinach leaves

Kobayashi, Yoshitaka; Köster, S.; Heber, U.

doi:10.1016/0005-2728(82)90117-7

Cited by 55 publications

(32 citation statements)

References 27 publications

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“…The ~-peak at 554 nm demonstrates that all phases of the kinetics are attributed to absorbance changes of cytochrome f. Furthermore the spectra point out the light-scattering signal at 535 nm, which is due to the formation of the proton gradient [12,13], and the electrochromic shift at 520 nm [29]. Interferences of other components are small compared to measurements of the cytochrome f signals in the soret band at 422 nm.…”

Section: Resultsmentioning

confidence: 99%

Regulation of the photosynthetic electron transport during dark-light transitions by activation of the ferredoxin-NADP+-oxidoreductase in higher plants

Rühle¹,

Pschorn²,

Wild³

1987

Photosynth Res

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Absorbance changes associated with the oxidation and reduction of cytochrome f belong to the classical observations about the interaction of the two photosystems. A complex induction pattern of cytochrome f oxidation results, if both photosystems are excited simultaneously. This indicates a light-modulated regulation of the photosynthetic electron transport, which we examined for intact biological systems of decreasing complexity. The ferredoxin-NADP(+)-oxidoreductase (FNR) is suggested to be activated by light and inactivated in the dark. This is pointed out by the kinetics of variable fluorescence and by the influence of different artificial electron acceptors on the cytochrome f kinetics. The photoreduction of NADP(+) by carefully prepared thylakoids demonstrates the activation process directly.

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Section: Resultsmentioning

confidence: 99%

Regulation of the photosynthetic electron transport during dark-light transitions by activation of the ferredoxin-NADP+-oxidoreductase in higher plants

Rühle¹,

Pschorn²,

Wild³

1987

Photosynth Res

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“…It decreased as photosynthesis recovered (data not shown). Increased light scattering is known to be an indicator of an increased proton gradient across the thylakoid membrane (Heber 1969;K6ster and Heber 1982;Kobayashi et al 1982;Bilger et al 1988). An increase in the magnitude of the proton gradient, when light remains unchanged, indicates either SO2-dependent inhibition of ATP formation by the thylakoid ATPase or decreased consumption of ATP by SO2-inhibited carbon assimilation.…”

Section: Resultsmentioning

confidence: 98%

Inhibition of photosynthesis, acidification and stimulation of zeaxanthin formation in leaves by sulfur dioxide and reversal of these effects

Jovanović

Bilger

Heber

1993

Planta

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Abstract.Leaves of Pelargonium zonale L. and Spinacia oleracea L. were fumigated with high concentrations of SO2 for very short periods of time with the aim of first producing acute symptoms of damage and then observing repair. The response of different photosynthetic parameters to SO2 was monitored during and after fumigation. The following results were obtained: (1) Inhibition of COa assimilation in the light was accompanied by increased reduction of the quinone acceptor, QA, of photosystem II and by increased oxidation of the electrondonor pigment PToo of photosystem I. Increased control of photosystem II activity in the SO2-inhibited state was also indicated by increased light scattering and by increased non-photochemical quenching of chlorophyll fluorescence. Both are indicators of chloroplast energization. Apparently, SO2 did not decrease but rather increased energization of the chloroplast thylakoid system by light. (2) Accumulation of dihydroxyacetone phosphate, fructose-l,6-phosphate and ribulose-l,5-phosphate and a decrease of 3-phosphoglycerate and hexosephosphate indicated that SO2 inhibited enzymes of the Calvin cycle. (3) Stimulated postillumination CO2 evolution suggested that when photosynthesis declined respiration increased to provide energy for repair reactions. (4) Increased leaf absorbance at 505 nm indicated increased stimulation of zeaxanthin formation in thylakoid membranes under the influence of SO2. A similar increase in 505-nm absorbance could be induced by high Dedicated to Professor O.L. Lange on the occasion of his 65th birthday * On leave from the Centre for Multidisciplinary Sciences, University of Belgrade, Yugoslavia Abbreviations: Chl = chlorophyll; DHAP = dihydroxyacetone phosphate; FBP=fructose-l,6-bisphosphate; F6P=fructoce-6-phosphate; F, Fm, Fm', Fo, Fo'=ch!orophyll fluorescence levels; PGA = 3-phosphoglycerate; P7oo = primary donor of photosystem I; QA = primary quinone acceptor of photosystem II; qp = photochemical quenching of chlorophyll fluorescence; NPQ = non-photochemical quenching of chlorophyll fluorescence; RuBP=ribulose-1,5-bisphosphate Correspondence to: U. Heber concentrations of CO2. In darkened leaves, SO 2 did not produce changes in 505-nm absorbance. (5) While zeaxanthin formation was stimulated, changes in the fluorescence of the pH-indicating dye pyranine, which had been fed to the leaves, indicated acidification of the cytoplasm of leaf cells by SO 2. Maximum acid production by SO 2 required light. In contrast, cytoplasmic acidification of leaf cells by CO2 was similar in the light and in the dark. (6) Since zeaxanthin formation is known to depend on the acidification of the thylakoid lumen, SO2-dependent zeaxanthin formation indicated SO2-dependent acidification of the thylakoid lumen as the indirect result of cytoplasmic acidification by SO2. (7) Inhibition of photosynthesis and other effects of SO2 were fully reversible in the light. Detoxification of SO2 and reactivation of the photosynthetic apparatus were slow or absent in the dark. Light had a dua...

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“…Kobayashi et al [4] showed that fluorescnece was also related to ATP pool size. It would seem that the faster the rate of ATP use, the more Mg ~ is inside the thylakoids, stimulating fluorescence.…”

Section: Discussionmentioning

confidence: 99%

“…Changing the 02 pressure changes the ATP requirement for CO2 assimilation. Since the rate of ATP use will greatly affect the transthylakoid membrane potential and it is this that affects steady state room temperature fluorescence from leaves [4,5], fluorescence was plotted against ATP use rate as calculated from the model of Farquhar, yon Caemmerer and Berry [2] for the data of Figure 4. The results ( Figure 5) show that in either low or normal 02 pressure, fluorescence was a good indicator of ATP use rate.…”

Section: Fluorescence From Intact Leaves As Affected By Lightmentioning

confidence: 99%

Steady-state room temperature fluorescence and CO2 assimilation rates in intact leaves

Sharkey

1985

Photosynth Res

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Steady-state room temperature variable fluorescence from leaves was measured as a function of CO2 pressure in Xanthium strumarium L. and Phaseolus vulgaris L. Measurements were made in a range of light intensities, at normal and low O2 parital pressure and over a range of temperatures.At low CO2 pressure fluorescence increased with increasing CO2. At higher CO2 pressure fluorescence usually decreased with increasing CO2 but occasionally increased slightly. The transition CO2 pressure between the responses could be changed by changing light, O2 pressure, or temperature. This breakpoint in the fluorescence-CO2 curve was a reliable indicator of the transition between ribulose 1,5-bisphosphate (RuBP) saturated assimilation and RuBP regeneration limited assimilation. The fluorescence signal was not a reliable indicator of O2-insensitive assimilation in these C3 species.

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Light scattering, chlorophyll fluorescence and state of the adenylate system in illuminated spinach leaves

Cited by 55 publications

References 27 publications

Regulation of the photosynthetic electron transport during dark-light transitions by activation of the ferredoxin-NADP+-oxidoreductase in higher plants

Regulation of the photosynthetic electron transport during dark-light transitions by activation of the ferredoxin-NADP+-oxidoreductase in higher plants

Inhibition of photosynthesis, acidification and stimulation of zeaxanthin formation in leaves by sulfur dioxide and reversal of these effects

Steady-state room temperature fluorescence and CO2 assimilation rates in intact leaves

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