Light stress and photoprotection in <i>Chlamydomonas reinhardtii</i>

Erickson, Erika; Wakao, Setsuko; Niyogi, Krishna

doi:10.1111/tpj.12825

Cited by 312 publications

(291 citation statements)

References 207 publications

(334 reference statements)

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“…In addition, it has been reported that, in mixotrophic conditions, PSII is more photoprotected than in photoautotrophic conditions showing less 1 O 2 production . The decrease of 1 O 2 under mixotrophic growth might be responsible to some extent for the overall higher Chl/ cell content in mixotrophic compared with photoautotrophic conditions, as an accumulation of 1 O 2 is one of the main triggers for the down-regulation of the photosynthetic genes (Erickson et al, 2015).…”

Section: The Composition Of the Photosynthetic Apparatus Depends On Tmentioning

confidence: 99%

“…In order to survive and grow, these organisms have developed various photoacclimation mechanisms operating on different time scales that protect the cell from photodamage. In the green alga Chlamydomonas reinhardtii, these mechanisms vary from negative phototaxis and multicomponent nonphotochemical quenching (NPQ) to a number of physiological and biochemical changes (Erickson et al, 2015). C. reinhardtii cells are around 10 mm in diameter, and a large part of their total volume is occupied by a single horseshoe-shaped chloroplast (Sager and Palade, 1957).…”

mentioning

confidence: 99%

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Carbon Supply and Photoacclimation Cross Talk in the Green Alga Chlamydomonas reinhardtii

et al. 2016

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Photosynthetic organisms are exposed to drastic changes in light conditions, which can affect their photosynthetic efficiency and induce photodamage. To face these changes, they have developed a series of acclimation mechanisms. In this work, we have studied the acclimation strategies of Chlamydomonas reinhardtii, a model green alga that can grow using various carbon sources and is thus an excellent system in which to study photosynthesis. Like other photosynthetic algae, it has evolved inducible mechanisms to adapt to conditions where carbon supply is limiting. We have analyzed how the carbon availability influences the composition and organization of the photosynthetic apparatus and the capacity of the cells to acclimate to different light conditions. Using electron microscopy, biochemical, and fluorescence measurements, we show that differences in CO 2 availability not only have a strong effect on the induction of the carbon-concentrating mechanisms but also change the acclimation strategy of the cells to light. For example, while cells in limiting CO 2 maintain a large antenna even in high light and switch on energy-dissipative mechanisms, cells in high CO 2 reduce the amount of pigments per cell and the antenna size. Our results show the high plasticity of the photosynthetic apparatus of C. reinhardtii. This alga is able to use various photoacclimation strategies, and the choice of which to activate strongly depends on the carbon availability.

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Section: The Composition Of the Photosynthetic Apparatus Depends On Tmentioning

confidence: 99%

mentioning

confidence: 99%

Carbon Supply and Photoacclimation Cross Talk in the Green Alga Chlamydomonas reinhardtii

et al. 2016

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“…To avoid photodamage, photosynthetic light harvesting is regulated by nonphotochemical quenching (NPQ), which allows dissipation of harmful excess energy as heat through its qE (energy-dependent nonphotochemical quenching) component (1)(2)(3)(4)(5)(6). Specialized members of the light harvesting complex (LHC) protein family, such as Photosystem II Subunit S (PSBS) in higher plants or members of the LHC Stress-Related (LHCSR) family in mosses and algae, are central to qE (7)(8)(9)(10)(11).…”

mentioning

confidence: 99%

UV-B photoreceptor-mediated protection of the photosynthetic machinery in Chlamydomonas reinhardtii

Allorent

Lefebvre-Legendre

Chappuis

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Life on earth is dependent on the photosynthetic conversion of light energy into chemical energy. However, absorption of excess sunlight can damage the photosynthetic machinery and limit photosynthetic activity, thereby affecting growth and productivity. Photosynthetic light harvesting can be down-regulated by nonphotochemical quenching (NPQ). A major component of NPQ is qE (energy-dependent nonphotochemical quenching), which allows dissipation of light energy as heat. Photodamage peaks in the UV-B part of the spectrum, but whether and how UV-B induces qE are unknown. Plants are responsive to UV-B via the UVR8 photoreceptor. Here, we report in the green alga Chlamydomonas reinhardtii that UVR8 induces accumulation of specific members of the light-harvesting complex (LHC) superfamily that contribute to qE, in particular LHC Stress-Related 1 (LHCSR1) and Photosystem II Subunit S (PSBS). The capacity for qE is strongly induced by UV-B, although the patterns of qE-related proteins accumulating in response to UV-B or to high light are clearly different. The competence for qE induced by acclimation to UV-B markedly contributes to photoprotection upon subsequent exposure to high light. Our study reveals an anterograde link between photoreceptor-mediated signaling in the nucleocytosolic compartment and the photoprotective regulation of photosynthetic activity in the chloroplast.L ight is essential for photosynthesis, but absorption of excess light energy is detrimental. To avoid photodamage, photosynthetic light harvesting is regulated by nonphotochemical quenching (NPQ), which allows dissipation of harmful excess energy as heat through its qE (energy-dependent nonphotochemical quenching) component (1-6). Specialized members of the light harvesting complex (LHC) protein family, such as Photosystem II Subunit S (PSBS) in higher plants or members of the LHC Stress-Related (LHCSR) family in mosses and algae, are central to qE (7-11). Protonation of key residues in these proteins triggers qE in response to the acidification of the thylakoid lumen, which is coupled to photosynthetic electron transport (7, 9). Furthermore, the deepoxidation of violaxanthin to zeaxanthin, which is also activated by the acidification of the thylakoid lumen, enhances qE (12). In response to high levels of visible light, LHCSR3 protein accumulation is of major importance for qE capacity in Chlamydomonas reinhardtii (11). The induction of LHCSR3 expression under high light is thought to involve retrograde signaling, from the chloroplast to nuclear gene expression (13), and recent data show that the response is also dependent on the phototropin (PHOT) blue light photoreceptor (14).UV-B radiation is intrinsic to sunlight reaching the earth surface and is potentially damaging to living tissues. UV-B stress tolerance is induced through the specific activation of acclimation responses (15)(16)(17)(18)(19)(20). Plants sense UV-B radiation via the homodimeric UV-B photoreceptor UV Resistance Locus 8 (UVR8) (21-23) that is mainly localized in the cytosol ...

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“…It is known to consist of several components each with a different induction and relaxation time (Müller et al 2001, Erickson et al 2015. One of these components (qI) is described as a sustained thermal dissipation mechanism that is slowly reversible (range of hours)…”

Section: Possible Explanations For the Discrepancy Between The Model mentioning

confidence: 99%

“…probably due to a combination of photoprotection and photodamage (Müller et al 2001, Lambrev et al 2010, Erickson et al 2015. Reaching maximum dissipation capacity involves the biosynthesis of enzymes and pigments such as zeaxanthin.…”

Section: Possible Explanations For the Discrepancy Between The Model mentioning

confidence: 99%

Antenna size reduction in microalgae mass culture

Mooij¹

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Light stress and photoprotection in Chlamydomonas reinhardtii

Cited by 312 publications

References 207 publications

Carbon Supply and Photoacclimation Cross Talk in the Green Alga Chlamydomonas reinhardtii

Carbon Supply and Photoacclimation Cross Talk in the Green Alga Chlamydomonas reinhardtii

UV-B photoreceptor-mediated protection of the photosynthetic machinery in Chlamydomonas reinhardtii

Antenna size reduction in microalgae mass culture

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