Lights on: Dye dequenching reveals polymersome fusion with polymer, lipid and stealth lipid vesicles

Henderson, Ian; Collins, Aaron M.; Quintana, Hope; Montaño, Gabriel A.; Martinez, Jesse; Paxton, Walter F.

doi:10.1016/j.polymer.2015.12.014

Cited by 10 publications

(7 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To investigate the effect that charged lipids have on the ζ potential of neutral polymers, we prepared and measured the ζ potential of cationic, neutral, and anionic hybrid NVs including EO 22 Bd 33 /DOTAP, EO 22 Bd 33 /1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC), and EO 22 Bd 33 /DOPS of variable composition. It has been shown in previous studies that this combination of lipid and polymer produces nominally unilamellar NVs via thin-film hydration. ,− ,− ,− These vesicles were subjected to extrusion through polycarbonate membranes to diameters of ∼100 nm, with thicknesses of 5–10 nm . Furthermore, all of these lipids have the same dioleoyl tails, and hybrid lipid/polymer vesicles prepared from EO x Bd y and the dioleoyl lipid DOPC were determined to be well-mixed and homogeneous without observable phase segregation using fluorescence resonance energy-transfer microscopy and cryogenic electron microscopy (cryo-EM) …”

Section: Results and Discussionmentioning

confidence: 99%

Modulating and Modeling the Surface ζ Potential of Hybrid Lipid/Polymer Nanovesicles: Implications for Surface Modification and Drug Delivery

Willes

McFarland

Johnson

et al. 2022

ACS Appl. Nano Mater.

View full text Add to dashboard Cite

Hybrid lipid/polymer membranes offer superior tunability over lipid-only systems, for a wide range of potential applications in surface modification and drug delivery, including nanovesicles (NVs) for mRNAbased therapeutics. The polymeric component can be readily modified for specific properties, and the lipid-to-polymer composition can be adjusted for even finer control. However, these materials are still an emerging field of study, and their potential is not yet fully understood. Modulating and modeling the ζ potential of these membranes is a critical step toward controlling their surface interactions. In this study, we performed ζ potential analysis on NVs with diameters of ∼100 nm made from lipids (1,2-dioleoyl-3-trimethylammonium propane, 1,2-dioleoyl-sn-glycero-3-phospho-L-serine, and 1,2-dioleoyl-sn-glycero-3-phosphocholine) and amphiphilic block copolymers (PEO−PBd, PBd−PEO−NH 3 + , and PBd−PEO−COO − ) at various compositions. This study revealed that NVs composed of the polymer PEO−PBd are surprisingly resilient to attempts to change their ζ potential with the addition of anionic or cationic lipids, which could limit their utility for drug-delivery applications and strategies to modify their surface chemistry. To overcome these limitations and explain these results, we devised a simple predictive and explanatory model that allows estimation of the ζ potential of hybrid NVs containing charged lipids. Our results indicate that the location of the charged groups can be controlled in order to impact the visibility of those charged groups to the surrounding materials and tissues that synthetic NVs interact with. We anticipate that our approach will greatly facilitate the design and modulation of the ζ potential and other properties of polymer NVs and other biomimetic membrane materials for nanomedicine and nanobiosensors.

show abstract

Section: Results and Discussionmentioning

confidence: 99%

Modulating and Modeling the Surface ζ Potential of Hybrid Lipid/Polymer Nanovesicles: Implications for Surface Modification and Drug Delivery

Willes

McFarland

Johnson

et al. 2022

ACS Appl. Nano Mater.

View full text Add to dashboard Cite

show abstract

“…Meanwhile, only micrometer‐sized polymersomes can be optically accessed. Because of this, to further assess fusion, we monitored membrane mixing via the dequenching of a hydrophobic dye 1,1″‐dioctadecyl‐3,3,3″,3′‐tetramethylindotricarbocyanine iodide (DiR), which was previously used for PBd‐ b ‐PEO vesicles, [ 31 ] and also measured the decreasing FRET signal between 7‐nitrobenz‐2‐oxa‐1,3‐diazol‐4‐yl (NBD) and fluorescein (FITC) or lissamine rhodamine B sulfonyl (Rho). Membrane mixing of DiR‐LUVs and dye‐free LUVs results in dilution of the dye, which restores its fluorescence (Figure S14A, Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

“…Similar structures were observed in populations of PEO 16 -b-PBO 22 vesicles upon addition of 20 kDa PEO. [22] Higher DiR concentrations have been also shown to promote the fusion of liposomes to cells for staining purposes [32] and the weak size control of DiR-DOPC liposomes [31] may be also related to similar interactions. To circumvent the unfavorable effect of DiR, we next employed two FRET pairs in a similar assay based on dilution.…”

Section: Membrane Mixingmentioning

confidence: 99%

Fusion‐Induced Growth of Biomimetic Polymersomes: Behavior of Poly(dimethylsiloxane)‐Poly(ethylene oxide) Vesicles in Saline Solutions Under High Agitation

Marušič

Zhao

Otrin

et al. 2021

Macromol. Rapid Commun.

View full text Add to dashboard Cite

Giant unilamellar vesicles serve as membrane models and primitive mockups of natural cells. With respect to the latter use, amphiphilic polymers can be used to replace phospholipids in order to introduce certain favorable properties, ultimately allowing for the creation of truly synthetic cells. These new properties also enable the employment of new preparation procedures that are incompatible with the natural amphiphiles. Whereas the growth of lipid compartments to micrometer dimensions has been well established, growth of their synthetic analogs remains underexplored. Here, the influence of experimental parameters like salt type/concentration and magnitude of agitation on the fusion of nanometer‐sized vesicles made of poly(dimethylsiloxane)‐poly(ethylene oxide) graft copolymer (PDMS‐g‐PEO) is investigated in detail. To this end, dynamic light scattering, microscopy, and membrane mixing assays are employed, and the process at different time and length scales is analyzed. This optimized method is used as an easy tool to obtain giant vesicles, equipped with membrane and cytosolic biomachinery, in the presence of salts at physiological concentrations.

show abstract

“…32−34 Compared to bulk macroscopic structures of platinum, dense Pt nanoparticles stacking on vesicle surface with hollow lumens can reduce the cost and improve the efficiency in potential applications. 35,36 3.6. Catalytic Study of Au@Vesicles in Water.…”

Section: Resultsmentioning

confidence: 99%

Hybrid Vesicles with Alterable Fully Covered Armors of Nanoparticles: Fabrication, Catalysis, and Surface-Enhanced Raman Scattering

Huang

et al. 2016

Langmuir

View full text Add to dashboard Cite

This work reports on the facile preparation of hybrid polymer vesicles with alterable armors of metal nanoparticles by using a novel hyperbranched polymer vesicle as the templates. The vesicles were prepared through the aqueous self-assembly of a hyperbranched multiarm copolymers with many tertiary amino groups on the surface, which can electrostatically complexed or coordinated with metal ions like AuCl4(-), PtCl6(2-), and Ag(+) ions. Subsequently, the vesicles coated with metal ions can be in situ reduced into metal nanoparticles, through which a series of surface-engineered vesicles (Au@vesicles, Ag@vesicles, Pt@vesicles) with an advantage of fully covered metal nanoparticles on the surface could be readily prepared. The morphologies, structures, and formation mechanism of the as-prepared hybrid vesicles were carefully characterized, and the obtained hybrid vesicles also showed great potentials in catalysis and surface-enhanced Raman scattering (SERS) applications.

show abstract

Lights on: Dye dequenching reveals polymersome fusion with polymer, lipid and stealth lipid vesicles

Cited by 10 publications

References 37 publications

Modulating and Modeling the Surface ζ Potential of Hybrid Lipid/Polymer Nanovesicles: Implications for Surface Modification and Drug Delivery

Modulating and Modeling the Surface ζ Potential of Hybrid Lipid/Polymer Nanovesicles: Implications for Surface Modification and Drug Delivery

Fusion‐Induced Growth of Biomimetic Polymersomes: Behavior of Poly(dimethylsiloxane)‐Poly(ethylene oxide) Vesicles in Saline Solutions Under High Agitation

Hybrid Vesicles with Alterable Fully Covered Armors of Nanoparticles: Fabrication, Catalysis, and Surface-Enhanced Raman Scattering

Contact Info

Product

Resources

About