Lignan formation in hairy root cultures of Edelweiss (Leontopodium nivale ssp. alpinum (Cass.) Greuter)

Abstract

A hairy root line of Edelweiss (Leontopodium nivale ssp. alpinum (Cass.) Greuter) was obtained upon transformation with Agrobacterium rhizogenes strain ATCC15834. Elicitation of this line with silver nitrate, sucrose, methyl jasmonate and yeast extract at various concentrations in most cases resulted in a stimulation of lignan biosynthesis. Through elicitation with 6% sucrose the roots accumulated the pharmacologically active lignans leoligin and 5-methoxy-leoligin at levels of 0.0678% and 0.0372%, respectively, without significant growth inhibition. These lignan levels were comparable to those found in intact roots of cultivated Edelweiss. The biotechnological production of leoligin could be an attractive option for the continuous, field culture-independent production of the valuable secondary metabolites leoligin and 5-methoxy-leoligin.

In vitro plant tissue culture: means for production of biological active compounds

“…Some of the advantages provided by hairy root cultures include high growth rates without the need for plant growth regulators, genetically and biochemically stable cultures, and a similar capacity for production of secondary metabolites than cell suspension cultures (Guillon et al 2006;Georgiev et al 2012;Mora-Pale et al 2014). A wide range of natural products has been produced using this system, including lignans, steroids, anthraquinones and alkaloids (Doma et al 2012;Huang et al 2014;Pandey et al 2014;Wawrosch et al 2014). However, metabolite 1 3 production requires that the compound of interest be one that is normally synthesized within the roots of the source plant, so this limits the versatility of the hairy root system (Ochoa-Villarreal et al 2016).…”

Anti-Aging Effects of Leontopodium alpinum (Edelweiss) Callus Culture Extract through Transcriptome Profiling

“…It is known that the active compounds of edelweiss extracts between the aerial parts and roots are diverse [3]. For instance, the hairy roots of edelweiss produce pharmacologically-active lignans, such as leoligin and 5-methoxy-leoligin, which can be stimulated by several other components, such as silver nitrate, sucrose, methyl jasmonate, and yeast extract [9]. Furthermore, the metabolic patterns of 11 different Leontopodium species have been revealed by nuclear magnetic resonance (1H NMR) spectroscopy and liquid chromatography-mass spectrometry (LC-MS) in their taxonomy relationship [10].…”

Lignan accumulation in two-phase cultures of Taxus x media hairy roots

“…Lignans, such as podophyllotoxin and 5-methoxypodophyllotoxin, which are of special interest, since their derivatives etoposide and teniposide are used in cancer therapy (Bohlin and Rosén 1996), could be accumulated in considerable amounts in in vitro cultures of various species from Podophyllum and Linum genera (Petersen and Alfermann 2001;Fuss 2003;Majumder and Jha 2009). The enhancement of lignan production in in vitro culture systems of various species was achieved by optimization of culture conditions including elaboration of the most suitable for product improvement carbon source, phytohormones, culture conditions like light or dark regime, elicitor treatments (reviewed in Fuss 2003), developing of hairy root cultures (Kumar et al 2012;Bahabadi et al 2014;Wawrosch et al 2014;Gabr et al 2016Gabr et al , 2017Tashackori et al 2016;Chashimi et al 2016) and metabolic engineering approaches (reviewed in Satake et al 2015). There has been also one paper on in vitro cultivated Taxus x media cell suspension and shoots where pinoresinol has been determined and revealed solely in shoots (Mistrzak et al 2015).…”