Ligninolytic Enzyme Production by White Rot Fungi Podoscypha elegans Strain FTG4

Determination of Lignin Modifying Enzymes from Pleurotus ostreatus and Lentinus squarrosulus

“…The colour change observed i.e orange halo, red to reddish-brown and black in the middle of the Petri dishes this is in line with the reports of Kiiskinen et al, [10]; Viswanath et al, [27]; Atalla et al, [28]; Sharma et al, [25]; Thiribhuvanamala et al, (2017). The colour change orange, red to reddish-brown and black in the middle is usually as a result of the oxidation of guaiacol by the laccase enzyme [29,30,26]. Kiiskinen et al, [10] in their investigation using different media types found MEA to be an excellent media for isolating laccase producing fungi also reported an excellent correlation of the presence of laccase activity with guaiacol and polymeric dyes (RBBR and Poly R-478), unlike Tannic acid which showed low specificity.…”

Bioprospection of ligninolytic enzymes from marine origin filamentous fungi

“…The production capacity of lignolytic enzymes was determined from the oxidation of specific compounds present in the culture medium (Agrawal et al 2017), where the 0.1% (m / v) syringaldazine solution was used for Lacase (Lac) and the 2 mM MnSO₄ solution and 0.1% (w / v) phenol red (Sigma-Aldrich®) for Manganese peroxidase (MnP) according to guidelines adapted from the method described by Szklarz et al (1989) and Kuwahara et al (1984), respectively. One disc (1 cm Ø) of pure colonies from each fungus was inoculated in the center of the Petri dish containing the Bushnell Haas medium (BH) (Difco®, United States) plus 1% (v / v) of oil from the Recôncavo basin sterilized in UV for 15 min, for condition I and in the culture medium Sabouraud Dextrose Agar (SDA) (Kasvi®, Brazil) for condition II.…”