2019
DOI: 10.1016/j.funbio.2019.01.007
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Lignocellulolytic characterization and comparative secretome analysis of a Trichoderma erinaceum strain isolated from decaying sugarcane straw

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Cited by 9 publications
(5 citation statements)
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“…Considering that the T. erinaceum secretomes produced on plant biomass showed higher enzymatic activity in p NPG than T. reesei [ 45 ], we searched for BGLs in the T. erinaceum secretome and transcriptome data. Five BGLs classified in the GH3 family (seqs 9295, 9527, 3444, 7567, and 8100) were identified considering all secretomes.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Considering that the T. erinaceum secretomes produced on plant biomass showed higher enzymatic activity in p NPG than T. reesei [ 45 ], we searched for BGLs in the T. erinaceum secretome and transcriptome data. Five BGLs classified in the GH3 family (seqs 9295, 9527, 3444, 7567, and 8100) were identified considering all secretomes.…”
Section: Resultsmentioning
confidence: 99%
“…A Trichoderma erinaceum strain was isolated from decaying sugarcane straw samples in a second-generation (2G) ethanol plant, and the secretomes produced on a pretreated sugarcane straw (SCS)-containing medium showed higher specific activities of XLNs and β-glucosidases (BGLs) than the T. reesei CBS 130855 strain. Moreover, T. erinaceum secretomes applied to pretreated SCS degradation yielded a higher glucose concentration and lower cellobiose accumulation than T. reesei secretomes produced under the same conditions [ 45 ].…”
Section: Introductionmentioning
confidence: 99%
“…On the other hand, the consortium CS2 was predominated by Enterobacteriaceae ((78:00±26:68) %), followed by Clostridia ((3:6 ± 13:7) %), fungi ((0:07 ± 0:02) %), and other microorganisms ((18:33 ± 7:32) %) (Figure 3). Several microbial consortia-with cellulolytic activity-were isolated and selected from different ecosystems, such as soils, straws and composts (Haruta et al, 2002;Feng et al, 2011;Gao et al, 2014;Young et al, 2018;Da Silva et al, 2019). Moreover, the identification of the cellulolytic consortia-degrading filter papers, newspapers, printing papers and cotton-found a great microbial diversity, including several genera: grampositive bacteria (Brevibacillus and Clostridium), gram-negative bacteria (Enterobacteriaceae, Pseudoxanthomonas), obligate anaerobes (Clostridia, Bordetella, etc.…”
Section: Isolates Origine Type Gram's Staining Results Catalase Test ...mentioning
confidence: 99%
“…Desta maneira, a análise do secretoma fúngico tem ganhado grande visibilidade, uma vez que através destes estudos é possível conhecer as proteínas secretadas por diferentes microrganismos cultivados em condições distintas (15)(16)(17)(18)(19).…”
Section: Biodiversidade Fúngicaunclassified
“…Provavelmente, isto ocorreu para essa biomassa devido ao fato de SCB ser uma mistura de diferentes variedades cultivadas na Usina da Pedra (Serrana -SP) (Tabela 6), além de uma possível hidrólise incompleta dos polissacarídeos pelas enzimas.Para melhor compreensão do padrão de hidrólise de cada biomassa, foi realizada uma análise do secretoma de M. thermophilus e T. reesei RP698 cultivado em diferentes biomassas. Este tipo de análise tem sido uma estratégia bastante utilizada(16)(17)(18)(19), pois através dela é possível decifrar e compreender quais as proteínas podem estar envolvidas no processo de degradação da biomassa lignocelulósica.O secretoma foi obtido pelo cocultivo de M. thermophilus e T. reesei RP698 em PM, no biorreator BioFlo 310, nas condições padronizadas para aplicação do coquetel, durante 5 dias de cultivo. Este coquetel apresentou a maioria das enzimas requeridas para a degradação da parede celular da cana-de-açúcar (1,9,23,26,27), incluindo várias glicosil hidrolases (GH) e carboidrato esterases (CE), como era esperado devido à complexidade da biomassa utilizada (Tabela 18).…”
unclassified