Lignocellulose-Decomposing Bacteria and Their Enzyme Systems

Bayer, Edward A.; Shoham, Yuval; Lamed, Raphael

doi:10.1007/978-3-642-30141-4_67

Cited by 47 publications

(45 citation statements)

References 309 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…They partially have a more or less different cellulosome architecture, and those of, for instance, the ruminococci are even more elaborate than in C. thermocellum (Bayer et al 2013). Of them C. thermocellum, C. cellulolyticum, and R. albus had been detected in biogas plants (Wirth et al 2012), underscoring their potentially important role for cellulose degradation in the industrial process.…”

Section: The Enzyme Systems Used By Cellulolytic Bacteria In Biogas Pmentioning

confidence: 97%

The Role of Cellulose-Hydrolyzing Bacteria in the Production of Biogas from Plant Biomass

Zverlov

Köck

Schwarz

2014

Microorganisms in Biorefineries

View full text Add to dashboard Cite

Section: The Enzyme Systems Used By Cellulolytic Bacteria In Biogas Pmentioning

confidence: 97%

The Role of Cellulose-Hydrolyzing Bacteria in the Production of Biogas from Plant Biomass

Zverlov

Köck

Schwarz

2014

Microorganisms in Biorefineries

View full text Add to dashboard Cite

“…Indeed, in this bacterium, like in the large majority of aerobic microorganisms, the cellulolytic system is based on the secretion of individual, free enzymes. By contrast, a restricted number of anaerobic bacteria perform efficient degradation of plant biomass through the secretion of unique, large multienzyme complexes termed cellulosomes (23)(24)(25)(26). These complexes are based on a noncatalytic subunit called scaffoldin that binds to the insoluble substrate via a cellulose-specific carbohydrate-binding module (CBM).…”

Section: Significancementioning

confidence: 99%

Toward combined delignification and saccharification of wheat straw by a laccase-containing designer cellulosome

Davidi

Moraïs

Artzi

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

Efficient breakdown of lignocellulose polymers into simple molecules is a key technological bottleneck limiting the production of plant-derived biofuels and chemicals. In nature, plant biomass degradation is achieved by the action of a wide range of microbial enzymes. In aerobic microorganisms, these enzymes are secreted as discrete elements in contrast to certain anaerobic bacteria, where they are assembled into large multienzyme complexes termed cellulosomes. These complexes allow for very efficient hydrolysis of cellulose and hemicellulose due to the spatial proximity of synergistically acting enzymes and to the limited diffusion of the enzymes and their products. Recently, designer cellulosomes have been developed to incorporate foreign enzymatic activities in cellulosomes so as to enhance lignocellulose hydrolysis further. In this study, we complemented a cellulosome active on cellulose and hemicellulose by addition of an enzyme active on lignin. To do so, we designed a dockerin-fused variant of a recently characterized laccase from the aerobic bacterium Thermobifida fusca. The resultant chimera exhibited activity levels similar to the wild-type enzyme and properly integrated into the designer cellulosome. The resulting complex yielded a twofold increase in the amount of reducing sugars released from wheat straw compared with the same system lacking the laccase. The unorthodox use of aerobic enzymes in designer cellulosome machinery effects simultaneous degradation of the three major components of the plant cell wall (cellulose, hemicellulose, and lignin), paving the way for more efficient lignocellulose conversion into soluble sugars en route to alternative fuels production.

show abstract

“…Saccharolytic fermenters, e.g. Spirochaeta, Clostridium and Porphyromonadaceae, are often located close to cellulolytic fermenters that are attached to cellulose fibers in anoxic environments (Leschine, 1995;Bayer et al, 2006;Li et al, 2009). These saccharolytic fermenters keep the concentration of cellobiose low and prevent inhibition of the cellulase system which results in enhanced cellulose hydrolysis (Ljungdahl and Eriksson, 1985;Pohlschroeder et al, 1994).…”

Section: Bacterial Taxa Linked To the Overall Degradation Of Cellulosementioning

confidence: 99%

“…Cellulose is hydrolysed under anoxic conditions to soluble cellodextrins, cellobiose or glucose by cellulase systems (cellulosomes) of cellulolytic fermenters (Lynd et al, 2002). Saccharolytic fermenters compete with cellulolytic fermenters for cellobiose and glucose, thereby keeping the concentration of cellulose-derived sugars at low non-inhibitory concentrations and enhancing cellulose degradation (Stanton and Canale-Parola, 1980;Pohlschroeder et al, 1994;Leschine, 1995;Bayer et al, 2006). In the absence of alternative electron acceptors (e.g.…”

Section: Introductionmentioning

confidence: 99%

Temperature impacts differentially on the methanogenic food web of cellulose‐supplemented peatland soil

Schmidt

Horn

Kolb

et al. 2014

Environmental Microbiology

View full text Add to dashboard Cite

The impact of temperature on the largely unresolved intermediary ecosystem metabolism and associated unknown microbiota that link cellulose degradation and methane production in soils of a moderately acidic (pH 4.5) fen was investigated. Supplemental [(13) C]cellulose stimulated the accumulation of propionate, acetate and carbon dioxide as well as initial methane production in anoxic peat soil slurries at 15°C and 5°C. Accumulation of organic acids at 15°C was twice as fast as that at 5°C. 16S rRNA [(13) C]cellulose stable isotope probing identified novel unclassified Bacteria (79% identity to the next cultured relative Fibrobacter succinogenes), unclassified Bacteroidetes (89% identity to Prolixibacter bellariivorans), Porphyromonadaceae, Acidobacteriaceae and Ruminococcaceae as main anaerobic degraders of cellulose-derived carbon at both 15°C and 5°C. Holophagaceae and Spirochaetaceae were more abundant at 15°C. Clostridiaceae dominated the degradation of cellulose-derived carbon only at 5°C. Methanosarcina was the dominant methanogenic taxa at both 15°C and 5°C. Relative abundance of Methanocella increased at 15°C whereas that of Methanoregula and Methanosaeta increased at 5°C. Thaumarchaeota closely related to Nitrosotalea (presently not known to grow anaerobically) were abundant at 5°C but absent at 15°C indicating that Nitrosotalea sp. might be capable of anaerobic growth at low temperatures in peat.

show abstract

Lignocellulose-Decomposing Bacteria and Their Enzyme Systems

Cited by 47 publications

References 309 publications

The Role of Cellulose-Hydrolyzing Bacteria in the Production of Biogas from Plant Biomass

The Role of Cellulose-Hydrolyzing Bacteria in the Production of Biogas from Plant Biomass

Toward combined delignification and saccharification of wheat straw by a laccase-containing designer cellulosome

Temperature impacts differentially on the methanogenic food web of cellulose‐supplemented peatland soil

Contact Info

Product

Resources

About