Lignocellulose degradation and production of lignin modifying enzymes by Schizophyllum commune IBL-06 in solid-state fermentation

Asgher, Muhammad; Wahab, Abdul Wahid; Bilal, Muhammad; Iqbal, Hafiz M.N.

doi:10.1016/j.bcab.2016.04.003

Cited by 105 publications

(25 citation statements)

References 48 publications

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“…In addition, some non-phenolic substrates, such as aromatic amines and carboxylic acids, and their derivatives can also be catalytically oxidized by laccase (Bourbonnais and Paice 1990;Eggert et al 1997;Polak and Jarosz-Wilkolazka 2012). As a cost-effective, industrial ideal green catalyst, laccase is widely applied in various fields of industry (Asgher et al 2016Bilal et al 2017a,b). Lignin contains a phenolic hydroxyl group, making it a suitable substrate for laccase oxidation.…”

Section: Introductionmentioning

confidence: 99%

Effect of Common Metal Ions and Anions on Laccase Catalysis of Guaiacol and Lignocellulosic Fiber

Zhou¹,

Dong²,

Wang³

et al. 2017

BioResources

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The effects of 12 common metal ionic compounds on the laccase catalytic activity in reactions using guaiacol as the substrate was determined using spectrophotometry. Furthermore, the influence of several metal ionic compounds on the generation of reactive oxygen species (ROS) by oxidation of lignin in jute fiber under laccase catalysis was studied by electron paramagnetic resonance (EPR) spectroscopy using N-tert-butylalpha-phenylnitrone (PBN) as the spin-trapping agent. , and Ag + exhibited either strong inhibitory effects on the catalysis of the substrate or a destructive effect on the structure of laccase itself. Furthermore, the results showed that an appropriate concentration of Cu 2+ helped to promote the thermal stability of laccase during the enzymatic reaction. This study could help researchers to avoid the use of inhibitory exogenous metal ions and anions in the application of laccase and to maximize the value of laccase.

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Section: Introductionmentioning

confidence: 99%

Effect of Common Metal Ions and Anions on Laccase Catalysis of Guaiacol and Lignocellulosic Fiber

Zhou¹,

Dong²,

Wang³

et al. 2017

BioResources

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“…The major problem associated with enzymatic delignification is low enzyme production and activity. Enhancing the culturing conditions may however help to increase the activity and the yield of these enzymes [36].…”

Section: Biological Pretreatmentmentioning

confidence: 99%

Potential of Weed Biomass for Bioethanol Production

Premjet¹

2019

Fuel Ethanol Production From Sugarcane

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Lignocellulosic biomass from weedy plants represents a potential alternative feedstock for economic production of bioethanol. Large numbers of weedy plant species are growing all over the world. Characteristics such as high dry matter yield, low water and nutrient requirements for growth, and cellulose contents make weedy plants very attractive as feedstock for bioethanol production. However, like other lignocellulosic feedstock, the complex structure presents resistance and recalcitrance to processes of conversion to bioethanol. Several weedy plants have been studied to determine their physical characteristics and suitability for bioethanol production. Different conversion techniques have been employed to increase monomer sugars and hence bioethanol yield. This chapter discusses processes and current research activities in bioconversion of weed biomass to bioethanol.

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“…They often face harsh conditions in industrial processes, such as high temperature, high salt concentration, and extremely acidic or alkaline pH [9]. Low yield and enzyme instability are important factors preventing large-scale application of laccases in biotechnology [10]. To this end, discovery of fungal-producing laccases with high yield and improved stabilities is necessary for industrial utilizations.…”

Section: Introductionmentioning

confidence: 99%

Screening, Production and Partial Characterization of a Thermostable Laccase from Trametes sp. Isolate B7 with Biotechnological Potentials

Ado

Amande

Ebah

et al. 2019

BJI

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The search for efficient and green oxidation technologies has increased interest in utilization of laccases in non conventional methods. Laccases catalyze a wide range of substrates due to low substrate specificity and strong oxidative potentials. Challenges to large-scale enzyme utilization include, low enzyme activity and instability which restrict use in many areas of biotechnology. In the study, 59 fungi comprising Aspergillus niger (40%), Trichoderma harzianum (31%), Aspergillus flavus (9.0%), Trichoderma viride (5.0%), Fusarium oxysporum (5.0%), Rhizopus stolonifer (5.0%), Trametes sp. (3.0%) and Aspergillus nidulans (2.0%) were isolated and screened for laccase production. Plate screening test showed 57.5%, 34.0% and 8.5% of fungi were laccase-positive on ABTS, Guaiacol, and α-naphthol agar respectively. Isolates were further screened in liquid cultures, and the highest laccase producer identified molecularly. Trametes sp isolate B7 was selected for solid state fermentation (SSF). Laccase production in SSF was highest at pH 5.0 (2356 U/mL). The purified laccase showed high activity (pH 3.0 - 6.0) and stability (pH 3.0 - 8.5) using ABTS. It was active (20 - 80°C) and thermostable (30 - 80°C) with optimum stability at 70°C (100% for 1 hour). The percentage decolourization of Phenol red were 28% and 36% using 1000 U/mL and 2000 U/mL crude laccases respectively. Similarly, RBBR (100%), Congo red (75%) and Malachite green (62%), and 77.4%, 64% and 28% were decolourized using 1000 U/mL and 2000 U/mL crude laccases respectively. ABTS agar was very reliable in large-scale screening for laccase which possessed thermostable property and degraded synthetic dyes without use of enzyme mediators. These attribute made the enzyme suitable for application in industry and biotechnology.

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Lignocellulose degradation and production of lignin modifying enzymes by Schizophyllum commune IBL-06 in solid-state fermentation

Cited by 105 publications

References 48 publications

Effect of Common Metal Ions and Anions on Laccase Catalysis of Guaiacol and Lignocellulosic Fiber

Effect of Common Metal Ions and Anions on Laccase Catalysis of Guaiacol and Lignocellulosic Fiber

Potential of Weed Biomass for Bioethanol Production

Screening, Production and Partial Characterization of a Thermostable Laccase from Trametes sp. Isolate B7 with Biotechnological Potentials

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