Lignocellulose‐degrading enzymes

Jaramillo, Paula Marcela Duque; Gomes, Helder Andrey Rocha; Monclaro, Antonielle Vieira; Silva, Caio O. G.; Filho, Edivaldo Ximenes Ferreira

doi:10.1002/9781118958308.ch6

Cited by 22 publications

(7 citation statements)

References 58 publications

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“…59 The associated microbial community can fragment and break down these biopolymers especially polysaccharides by encoding Carbohydrate-Active Enzymes or CAZymes, which find various industrial and biotechnological applications such as bioconversion of POW biomass into sugars, fuels and other bioproducts of interest. 24,60,61 The metagenomic analysis performed by Rubio-Portillo et al 24 permits to identify the microbial community associated with POW. Alphaproteobacteria, Gammaproteobacteria, Bacteroidetes and Cyanobacteria were the dominant members, while Pseudoalteromonas was the dominant genus, followed by Alteromonas, Labrenzia and Aquimarina.…”

Section: Valorization Of Pow As a Potential Source Of Carbohydrate Ac...mentioning

confidence: 99%

Untitled

2024

BBIJ

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In the Mediterranean region, every summer the beaches have to be cleaned and millions of tons of Posidonia oceanica waste (POW) is often removed and not exploited. POW is mainly composed of fibrous materials consisting of cellulose and hemicellulose and lignin, as well as a significant percentage of ash and phenolic compounds. A solution to this ecological problem could be the valorization of this available and renewable biomass for the production of environmentally friendly industrial products. This mini-review focuses on the utilization of POW as a valuable biomass resource. In particular, seven POW valorization treatment approaches are discussed in this paper with a focus on bioenergy and biomaterials. The use of cleaner technologies can offer improved performance and an asset for contribution on sustainable POW management.

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Section: Valorization Of Pow As a Potential Source Of Carbohydrate Ac...mentioning

confidence: 99%

Untitled

2024

BBIJ

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“…Carbohydrate binding modules (CBMs) are non-catalytic modules, generally defined as accessory CAZymes, and their main role is to recognise the substrates by binding carbohydrates [5]. Several different industrial sectors, such as food industries and biorefineries, have been benefiting from various bacterial and fungal strains for CAZymes provision [6]. A better understanding of microbial physiology and ecology could result in enlarging the pool of available strains for scientific and industrial use, such as recombinant design and enzyme cocktail development [7].…”

Section: Introductionmentioning

confidence: 99%

Comparative genomic analysis ofPlanctomycetotapotential towards complex polysaccharide degradation identifies phylogenetically distinct groups of biotechnologically relevant microbes

Klimek,

Herold,

Calusinska

2024

Preprint

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The outstanding hydrolytic potential of thePlanctomycetotaphylum for complex polysaccharide degradation has recently been acknowledged based on the numerous carbohydrate-active enzymes (CAZymes) encoded in their genomes. However, mainly members of thePlanctomycetiaclass have been characterised up to now, and little is known about the degrading capacities of the otherPlanctomycetota. Our in-depth characterisation of the available planctomycetotal genomic resources increased our knowledge of the carbohydrolytic capacities ofPlanctomycetota. We showed that this single phylum encompasses a wide variety of the currently known CAZyme diversity assigned to glycoside hydrolase families, and that many members are characterised by a high versatility towards complex carbohydrate degradation, including lignocellulose. We also highlighted members of theIsosphaerales, Pirellulales, SedimentisphaeralesandTepidisphaeralesorders as having the highest encoded hydrolytic potential of thePlanctomycetota. Furthermore, members of a yet uncultivated group affiliated toPhycisphaeraleswere identified as an interesting source of novel, lytic polysaccharide monooxygenases that could boost lignocellulose degradation. Surprisingly, manyPlanctomycetotafrom anaerobic digestion reactors were shown to encode CAZymes targeting algal polysaccharides – this opens new perspectives for algal biomass valorisation in biogas processes. Our study provides a new perspective on planctomycetotal carbohydrolytic potential, highlighting distinct phylogenetic groups which could provide a wealth of diverse, potentially novel CAZymes of industrial interest.

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“…Microbial cellulase have become the focal biocatalyst due to their complex nature and wide range of industrial applications (Kuhad et al 2011), such as pulp and paper, textile, fruit juice extraction, animal feed additives, and bioethanol production (Bhat 2000). The application of cellulase for industrial applications is increasing until it reaches 20% of global market demand (Jaramillo et al 2015), with the total sales in The United States reaching to 400 million USD per year (Beilen and Li 2002). Likewise, in Indonesia, the use of cellulase has increased by 7% each year (Utami et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Leclercia adecarboxylata C12, The Newly Isolated Cellulose-degrading Bacteria from Indonesian Coffee Pulp

2023

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Culturable cellulose-degrading microorganisms were collected from Arabica coffee pulp in East Java, Indonesia. Fifty isolates were obtained, and thirty-three isolates showed hydrolyzing zone on Carboxy Methyl Cellulose agar plates after Congo-Red staining. The highest specific CMCase activity was observed by isolates C12, identified as Leclercia adecarboxylata based on 16S-rRNA gene sequence analysis. SDS-PAGE of Leclercia adecarboxylata C12 cellulase revealed two bands with a molecular mass of 95.49 and 81.28 kDa, respectively. Activity gel analysis showed the cellulolytic ability of Leclercia adecarboxylata C12 cellulase by clear zone formation. The optimal CMCase activity was achieved at 50°C and pH 9, and the activity retained 47% of its initial activity after incubation at 50°C for 90 minutes. The purified enzyme remains stable from pH 5 to 10, with 77% of its maximum activity. The activity of CMCase was stimulated by the presence of K+, Ca2+, Mg2+, and Fe3+, while SDS and EDTA reduced its activity. The current study shows that the thermostable-alkalophilic cellulase produced by Leclercia adecarboxylata C12 is very promising for industrial applications.

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Lignocellulose‐degrading enzymes

Cited by 22 publications

References 58 publications

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Comparative genomic analysis ofPlanctomycetotapotential towards complex polysaccharide degradation identifies phylogenetically distinct groups of biotechnologically relevant microbes

Leclercia adecarboxylata C12, The Newly Isolated Cellulose-degrading Bacteria from Indonesian Coffee Pulp

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