Lignocellulosic pretreatment-mediated phenolic by-products generation and their effect on the inhibition of an endo-1,4-β-xylanase from Thermomyces lanuginosus VAPS-24

Mathibe, Brian N.; Malgas, Samkelo; Radosavljevic, Layla; Kumar, Vishal; Shukla, Pratyoosh; Pletschke, Brett I.

doi:10.1007/s13205-020-02343-w

Cited by 11 publications

(2 citation statements)

References 35 publications

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“…Similar work also observed that the carboxylic acid groups of phenolics and lignin exhibited less inhibitory effects than phenolics with aldehyde or alcohol groups due to the decreased lignin-cellulase binding (Qin et al, 2016;Zhai et al, 2018). More recent work done by Mathibe et al (2020) used several model lignin derivatives to study their effects on endo-1,4-β-xylanase and found that the presence of carbonyl groups in phenolics exhibited more substantial inhibitory effects than hydroxyl groups on endo-1,4β-xylanase, resulting in over 50% reduction in activity at a concentration as low as 0.5 mg/ml.…”

Section: Inhibitory Impact Of Combined Liquid Hydrolysate and Pretrea...mentioning

confidence: 52%

Inhibition of cellulase activity by liquid hydrolysates from hydrothermally pretreated soybean straw

Kim¹,

Ji²,

Jackson³

et al. 2022

Front. Chem. Eng.

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The one-pot biomass conversion process is a promising strategy to minimize potential product loss and reduce processing costs. However, this strategy has technical limitations due to the inhibitory effects of biomass components like lignin as well as the generated inhibitors (e.g., furans, phenols) during biomass processing. In this study, the inhibitory effects of liquid hydrolysates formed by hydrothermal pretreatment of soybean straw with either sodium hydroxide (NaOH) or hydrogen peroxide (H2O2) on cellulolytic enzyme activity were investigated. Hydrothermal pretreatment of soybean straw (10% w/v) was carried out with either sodium hydroxide (1% v/v) or hydrogen peroxide (1% v/v) at 121°C for 60 min to evaluate the effect of water-soluble inhibitors released from soybean pretreatment on cellulolytic enzyme activity. The fraction of cellulose in pretreated solids (1% w/v glucan) was enzymatically hydrolyzed for 72 h with 45 IU/g glucan (corresponding to 25 mg enzyme protein/g glucan) in the presence of either buffer or liquid hydrolysate generated from the pretreatments. Hydrolysis of NaOH and H2O2 pretreated solids resulted in 57% and 39% of glucose yields in buffer, respectively. In the presence of the liquid hydrolysates, NaOH and H2O2 pretreated biomass showed 20% and 30% glucose yield, respectively, indicating the enzyme suppression by inhibitors in the liquid hydrolysates. Of the enzyme activities in hydrolysates tested, NaOH hydrolysate showed a higher inhibitory effect on enzyme activities (mainly β-glucosidase) compared to H2O2 liquid, where enzyme deactivation has a first-order correlation and the manner in which the vacuum-filtered inhibitors were generated from pretreated soybean straw.

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Section: Inhibitory Impact Of Combined Liquid Hydrolysate and Pretrea...mentioning

confidence: 52%

Inhibition of cellulase activity by liquid hydrolysates from hydrothermally pretreated soybean straw

Kim¹,

Ji²,

Jackson³

et al. 2022

Front. Chem. Eng.

View full text Add to dashboard Cite

show abstract

“…During pretreatment, lignocellulosic biomass is degraded into cellulose, hemicellulose, and lignin, using chemical or physical methods. During enzymatic hydrolysis, the pretreated biomass is hydrolyzed using several enzymes, such as Acremonium cellulase [7], Trichoderma cellulase [7], and xylanase [8], which generate the hydrolysate containing mixed sugars. Finally, the hydrolysate is used as the carbon source during fermentation.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Oil Palm Empty Fruit Bunch Hydrolysate

2021

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Malaysia is the second largest palm oil producer and exporter globally. When crude palm oil is produced in both plantations and oil processing mills, a large amount of oil palm empty fruit bunch (OPEFB) is simultaneously produced as a waste product. Here, we describe the preparation of hydrolysate from OPEFB. After OPEFB was hydrothermally treated at 180–200 °C, the resultant liquid phase was subjected to high-performance liquid chromatography analysis, while the solid phase was used for acidic and enzymatic hydrolysis. Hemicellulose yield from the acid-treated solid phase decreased from 153 mg/g-OPEFB to 27.5 mg/g-OPEFB by increasing the hydrothermal treatment temperature from 180 to 200 °C. Glucose yield from the enzyme-treated solid phase obtained after hydrothermal treatment at 200 °C was the highest (234 ± 1.90 mg/g-OPEFB, 61.7% production efficiency). In contrast, xylose, mannose, galactose, and arabinose yields in the hydrolysate prepared from the solid phase hydrothermally treated at 200 °C were the lowest. Thus, we concluded that the optimum temperature for hydrothermal pretreatment was 200 °C, which was caused by the low hemicellulose yield. Based on these results, we have established an effective method for preparing OPEFB hydrolysates with high glucose content.

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Biochemical Conversion of Cellulose

Kim

2022

Biomass Utilization: Conversion Strategies

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Lignocellulosic pretreatment-mediated phenolic by-products generation and their effect on the inhibition of an endo-1,4-β-xylanase from Thermomyces lanuginosus VAPS-24

Cited by 11 publications

References 35 publications

Inhibition of cellulase activity by liquid hydrolysates from hydrothermally pretreated soybean straw

Inhibition of cellulase activity by liquid hydrolysates from hydrothermally pretreated soybean straw

Preparation of Oil Palm Empty Fruit Bunch Hydrolysate

Biochemical Conversion of Cellulose

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