Limited expression of nuclear pore membrane glycoprotein 210 in cell lines and tissues suggests cell-type specific nuclear pores in metazoans

Olsson, Magnus; Schéele, Susanne; Ekblom, Peter

doi:10.1016/j.yexcr.2003.09.014

Cited by 78 publications

(70 citation statements)

References 37 publications

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“…Surviving gp210(tm2320) mutant nematodes had no detectable full-length gp210. This suggests that gp210 is not absolutely essential and confirm previous observations in mouse that neither cell viability nor the stable association of NUP107 and POM121 with the NPC during interphase are affected in cells that do not express gp210 (Eriksson et al, 2004;Olsson et al, 2004). Similarly, human cultured cells depleted of gp210 by RNAi were viable (Stavru et al, 2006b).…”

Section: Discussionsupporting

confidence: 84%

A role for gp210 in mitotic nuclear-envelope breakdown

Galy

Antonin

Jaedicke

et al. 2008

Journal of Cell Science

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Section: Discussionsupporting

confidence: 84%

A role for gp210 in mitotic nuclear-envelope breakdown

Galy

Antonin

Jaedicke

et al. 2008

Journal of Cell Science

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“…Critical gene regulatory functions of Nups are also supported by their association with diseases and cell type-specific NPC composition observed in multicellular organisms (Olsson et al 2004;Gomez-Cavazos and Hetzer 2012). Mutations in Nups are responsible for a number of developmental disorders and cancer, including cases of acute myeloid leukemia (AML).…”

Section: Gene Expression Regulation In Metazoansmentioning

confidence: 99%

Nuclear pore proteins and the control of genome functions

2015

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Nuclear pore complexes (NPCs) are composed of several copies of ∼30 different proteins called nucleoporins (Nups). NPCs penetrate the nuclear envelope (NE) and regulate the nucleocytoplasmic trafficking of macromolecules. Beyond this vital role, NPC components influence genome functions in a transport-independent manner. Nups play an evolutionarily conserved role in gene expression regulation that, in metazoans, extends into the nuclear interior. Additionally, in proliferative cells, Nups play a crucial role in genome integrity maintenance and mitotic progression. Here we discuss genome-related functions of Nups and their impact on essential DNA metabolism processes such as transcription, chromosome duplication, and segregation.

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“…Tissue-specific expression of two Nups has been detected during mouse development (117,153), although the molecular consequences of this altered NPC composition on signaling VOL. 8, 2009 MINIREVIEW 1821…”

Section: Alteration Of Fg-nup Composition In Disease and Developmentmentioning

confidence: 99%

Flexible Gates: Dynamic Topologies and Functions for FG Nucleoporins in Nucleocytoplasmic Transport

2009

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The nuclear envelope is a physical barrier between the nucleus and cytoplasm and, as such, separates the mechanisms of transcription from translation. This compartmentalization of eukaryotic cells allows spatial regulation of gene expression; however, it also necessitates a mechanism for transport between the nucleus and cytoplasm. Macromolecular trafficking of protein and RNA occurs exclusively through nuclear pore complexes (NPCs), specialized channels spanning the nuclear envelope. A novel family of NPC proteins, the FG-nucleoporins (FG-Nups), coordinates and potentially regulates NPC translocation. The extensive repeats of phenylalanine-glycine (FG) in each FG-Nup directly bind to shuttling transport receptors moving through the NPC. In addition, FG-Nups are essential components of the nuclear permeability barrier. In this review, we discuss the structural features, cellular functions, and evolutionary conservation of the FG-Nups.Subcellular compartmentalization of eukaryotic cells into organelles imparts functional and spatial separation of essential cellular processes. Interorganellar communication, however, is required to coordinate activities within the cell. The movement of molecules between the cytoplasm and a given organelle is accomplished by the use of a regulatory transport pore(s) embedded in the organelle membrane. One of the most complex molecular translocons is the nuclear pore complex (NPC), which mediates all traffic of macromolecules in and out of the nucleus.NPCs are large, selective channels that regulate the nucleocytoplasmic transport of macromolecules but are permeable to the movement of ions, small metabolites, and small proteins by free diffusion. The ability of the NPC to rapidly transport specific macromolecules and coincidently selectively preclude other molecules from entering the nucleus is one of the mysteries of this biological machine. To overcome the permeability barrier, each cargo greater than ϳ40 kDa must display a nuclear localization sequence (NLS) or nuclear export sequence (NES). The respective NLS or NES is recognized and bound by a specific transport receptor, of which many exist in eukaryotic cells.Transport receptors interact with a subset of NPC proteins to mediate translocation and, as such, serve as a molecular bridge between NPC proteins and cargoes to allow efficient nuclear import and export. A unique family of NPC proteins is directly involved, and are designated the FG-nucleoporins (FG-Nups). The FG-Nups are characterized by domains with extensive repeats of phenylalanine-glycine (FG), and these proteins have specific and essential roles in transport through the NPC (discussed below). Recent work has offered many insights into the biophysical nature of the FG-Nups. Structural aspects of interactions between FG-Nups and transport receptors have been resolved, and regulatory roles of FG-Nups in transport, disease, and development have been discovered. Importantly, understanding the structural, functional, and regulatory properties of FG-Nups has provided new ...

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Limited expression of nuclear pore membrane glycoprotein 210 in cell lines and tissues suggests cell-type specific nuclear pores in metazoans

Cited by 78 publications

References 37 publications

A role for gp210 in mitotic nuclear-envelope breakdown

A role for gp210 in mitotic nuclear-envelope breakdown

Nuclear pore proteins and the control of genome functions

Flexible Gates: Dynamic Topologies and Functions for FG Nucleoporins in Nucleocytoplasmic Transport

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