LINC00511 promotes cervical cancer progression by regulating the miR-497-5p/MAPK1 axis

Abstract

Background Long non-coding RNA (lncRNA) exhibits a crucial role in multiple human malignancies. The expression of lncRNA LINC00511, reportedly, is aberrantly up-regulated in several types of tumors. Our research was aimed at deciphering the role and mechanism of LINC00511 in the progression of cervical cancer (CC). Method Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to quantify the expression levels of LINC00511, miR-497-5p and MAPK1 mRNA in CC tissues and cell lines. Cell counting kit-8 (CCK-8), 5-bromo-2’-deoxyuridine (BrdU) and Transwell assays were conducted for detecting the proliferation, migration and invasion of CC cells. Dual-luciferase reporter gene experiments were performed to verify the targeting relationships amongst LINC00511, miR-497-5p and MAPK1. Besides, MAPK1 expression in CC cells was detected via Western blot after LINC00511 and miR-497-5p were selectively regulated. Results Up-regulation of LINC00511 expression in CC tissues and cell lines was observed, which was in association with tumor size, clinical stage and lymph node metastasis of the patients. LINC00511 overexpression facilitated the proliferation, migration and invasion of CC cells, while opposite effects were observed after knockdown of LINC00511. Mechanistically, LINC00511 was capable of targeting miR-497-5p and up-regulating MAPK1 expression. Conclusion LINC00511/miR-497-5p/MAPK1 axis regulates CC progression.

Elucidating the mechanism of Hongjinshen decoction in the treatment of pulmonary fibrosis based on network pharmacology and molecular docking

“…[ 23 ] MAPK1 gene encodes mitogen-activated protein kinase, also known as extracellular signal-regulated kinase, a member of the MAPK family, which is involved in a variety of BPs in the body as a key protein kinase in the signaling cascade. [ 24 ] Liu et al proposed that the anti-fibrotic molecule LINC00636 could inhibit MAPK1 by promoting the overexpression of miR-450a-2-3p, which ultimately exerts an ameliorative effect on cardiac fibrosis in patients with atrial fibrillation. [ 25 ] Therefore, MAPK1 may be a potential therapeutic target for antifibrotic therapy.…”

MicroRNAs and Gene Regulatory Networks Related to Cleft Lip and Palate

“…hsa-miR-665-3p decreases cell viability by apoptosis or suppresses cell proliferation through downregulation of target genes in various cells, namely BCL2 in human lung adenocarcinoma cells [ 48 ], NHEG1 in human neuroblastoma [ 49 ], TRIM24 in human castration-resistant prostate cancer [ 50 ], and FZD4 in human oral squamous cell carcinoma [ 51 ]. In addition, hsa-miR-497-5p inhibits cell proliferation through downregulation of target genes in several human cancer cells, e.g., MAPK1 in cervical cancer cells [ 52 ], PDL1 or SLC7A5 in human colorectal cancer cells [ 53 ], and WNT3A in human nasopharyngeal carcinoma cells [ 54 ]. Thus, miR-124-3p, miR-655-3p, and miR-497-5p may play a key role in cell proliferation as tumor suppressors in cancers and CL/P inducers in development.…”

MicroRNAs as Potential Biomarkers in Gynecological Cancers

“…On the contrary, downregulation of miR-497-5p expression and the increase in LINC00511 expression induced MAPK1 expression. Thus, the LINC00511/miR-497-5p/MAPK1 axis affects the progression of cervical cancer [119].…”