LINC00963: A potential cancer diagnostic and therapeutic target

Xie, Zijun; Zhong, Chenming; Shen, Jinze; Jia, Yunhua; Duan, Shiwei

doi:10.1016/j.biopha.2022.113019

Cited by 20 publications

(10 citation statements)

References 54 publications

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“…LINC00963 can also control four downstream protein-coding genes directly. By participating in EGFR transactivation, LINC00963 particularly promotes the transition of prostate cancer through an androgen-dependent to an androgen-independent phase (Xie et al, 2022). Based on the study of Wu et al in 2020, LINC00963 was discovered to be up-regulated in BC patients, and its presence was correlated to metastasis to "lymph node, TNM stage, and differentiation grade".…”

Section: Discussionmentioning

confidence: 99%

LNC01089-LINC00963/miR-1244-5p/IGF1 ceRNA axis might regulate FOXO signaling pathway in breast cancer patients: a biomarker discovery investigation

Rezaei,

Marghmaleki,

Boroujeni

et al. 2023

Preprint

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Background Breast carcinoma (BC) ranks as one of the most prevalent illnesses among women, and a variety of factors, including inherited and environmental factors, can impact its start and progression. A variety of biological biomarkers (measurement of enzymes, hormones, and mRNA and microRNA expression patterns) have been identified for the prediction of poor prognosis and diagnosis of BC. In this study, we tried to analyze the expression patterns of mRNAs and long non-coding RNAs (lncRNAs) and find novel biomarkers for diagnosis and prognosis of BC during a systems biology approach. Methods Microarray analysis was performed to find novel potential BC biomarkers. Using miRWalk, lncRRIsearch, STRING, and Cytoscape, non-coding and protein interaction analysis was utilized and visualized. Pathway enrichment and gene ontology analyses were performed to find accurate biological mechanisms of selected RNAs. The correlation of lncRNA and mRNA expression level with the survival rate of BC patients was shown using GEPIA2. Expression level of miRNA was performed using ENCORI. Using qRT-PCR on 50 tumor samples compared to 50 control samples for validation of bioinformatics expression analyses and understanding of diagnosis capability of selected RNAs (using Receiver operating characteristic (ROC) analysis. Results IGF1 expression level had a significant reduction in BC, based on microarray and qRT-PCR experiments. LINC00963 and LNC01089 also have significant decrease in expression level, based on GEPIA2 and qRT-PCR. LNC01089 and LINC00963 could represent suitable BC diagnostic (depending on ROC analysis) and prognosis (clinicopathological analysis) biomarkers. The two mentioned lncRNAs have direct interaction with IGF1 mRNA. miR-1244-5p as a potential up-regulated oncogene of BC suppresses the expression level of LNC01089, LINC00963, and IGF1. IGF1 is a key modulator of the FOXO signaling pathway. The mentioned RNAs have a significant correlation with clinicopathological features of BC patients, including age, lymph node metastasis, and menopausal status. Conclusion LINC00963 and LNC01089, as the two potential tumor suppressors of BC, could regulate the FOXO signaling pathway through direct interaction with IGF1 mRNA. miR-1244-5p also might have a critical role in FOXO regulation through suppression of IGF1 and two mentioned lncRNAs.

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Section: Discussionmentioning

confidence: 99%

LNC01089-LINC00963/miR-1244-5p/IGF1 ceRNA axis might regulate FOXO signaling pathway in breast cancer patients: a biomarker discovery investigation

Rezaei,

Marghmaleki,

Boroujeni

et al. 2023

Preprint

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“…Safranal, a small molecule from saffron, has shown anticancer activity against HepG2 cells via oxidative stress-triggered protein destabilization and DNA damage apoptosis [ 25 ]. Moreover, mounting evidence has revealed that noncoding RNAs, such as LINC00963, show potent oncogenic effects via modulating the PI3K/AKT, Wnt, AMPK, and MAPK signaling pathways, thereby governing cell proliferation, migration, invasion, EMT, and apoptosis [ 26 ]. Adenine-induced AMPK activation has been implicated in the regulation of cell cycle progression and invasiveness of cancer cells [ 19 , 27 ]; however, the roles of oxidative stress and noncoding RNAs in adenine-mediated anticancer action need further investigation.…”

Section: Discussionmentioning

confidence: 99%

Adenine Combined with Cisplatin Promotes Anticancer Activity against Hepatocellular Cancer Cells through AMPK-Mediated p53/p21 and p38 MAPK Cascades

et al. 2022

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Cisplatin has been widely used in cancer treatments. Recent evidence indicates that adenine has potential anticancer activities against various types of cancers. However, the effects of the combination of adenine and cisplatin on hepatocellular carcinoma (HCC) cells remain sketchy. Here, our objective was to elucidate the anticancer activity of adenine in combination with cisplatin in HCC cells and its mechanistic pathways. Cell viability and cell cycle progression were assessed by the SRB assay and flow cytometry, respectively. Apoptosis was demonstrated by PI/annexin V staining and flow cytometric analysis. Protein expression, signaling cascade, and mRNA expression were analyzed by Western blotting and quantitative RT-PCR, respectively. Our results showed that adenine jointly potentiated the inhibitory effects of cisplatin on the cell viability of SK-Hep1 and Huh7 cells. Further investigation showed that adenine combined with cisplatin induced higher S phase arrest and apoptosis in HCC cells. Mechanically, adenine induced AMPK activation, reduced mTOR phosphorylation, and increased p53 and p21 levels. The combination of adenine and cisplatin synergistically reduced Bcl-2 and increased PUMA, cleaved caspase-3, and PARP in HCC cells. Adenine also upregulated the mRNA expression of p53, p21, PUMA, and PARP, while knockdown of AMPK reduced the increased expression of these genes. Furthermore, adenine also induced the activation of p38 MAPK through AMPK signaling, and the inhibition of p38 MAPK reduced the apoptosis of HCC cells with exposure to adenine combined with cisplatin. Collectively, these findings reveal that the combination of adenine and cisplatin synergistically enhances apoptosis of HCC cells, which may be attributed to the AMPK-mediated p53/p21 and p38 MAPK cascades. It suggests that adenine may be a potential adjuvant for the treatment of HCC in combination with cisplatin.

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“…In addition to the above-mentioned signalling mechanisms and physiological consequences triggered by the ET-1 axis, the ET-1 GPCR receptor can activate Epidermal Growth Factor Receptor (EGFR) through an unconventional signalling process known as signal crosstalk [9,82,83]. The tumorigenicity of the EGFR activation and signalling pathway has been long studied in depth in ovary cancer head and neck cancer, colorectal cancer and breast cancer [38,[83][84][85]. EGFR and its signalling are key regulators of cellular activities such as proliferation, differentiation, apoptosis, and migration [86,87].…”

Section: Role Of Et-1 Axis In Cancermentioning

confidence: 99%

The Role of Endothelin Axis in Cancer Treatment and Its Position in Therapeutic Strategies

GÜL¹

2023

jrp

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Endothelin and its two different cognate receptors have been implicated in a wide variety of pathophysiological conditions since it was first identified as a vasoactive local hormone.Especially in the last decade, the studies revealed that the aberrant expression of endothelin axis has critical importance in the cancer biology involving in neovascularization, cellular survival, metastasis as well as drug resistance, by taking part in different signalling mechanisms within the cell. This situation has attracted the attention of many scientists to investigate not only reveal the molecular mechanistic role of endothelin axis in cancer pathophysiology but also design new drugs targeting the endothelin axis. Further studies suggest that utilizing endothelin axis as a new diagnostic parameter to define malignant features and monitor the prognoses of cancer This study, which was compiled from both preclinical and clinical studies, was prepared to provide an overview of the potential role of the endothelin axis in cancer biology, as well as an overview of the drugs developed against the endothelin axis

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LINC00963: A potential cancer diagnostic and therapeutic target

Cited by 20 publications

References 54 publications

LNC01089-LINC00963/miR-1244-5p/IGF1 ceRNA axis might regulate FOXO signaling pathway in breast cancer patients: a biomarker discovery investigation

LNC01089-LINC00963/miR-1244-5p/IGF1 ceRNA axis might regulate FOXO signaling pathway in breast cancer patients: a biomarker discovery investigation

Adenine Combined with Cisplatin Promotes Anticancer Activity against Hepatocellular Cancer Cells through AMPK-Mediated p53/p21 and p38 MAPK Cascades

The Role of Endothelin Axis in Cancer Treatment and Its Position in Therapeutic Strategies

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