LINC01140 inhibits nonsmall cell lung cancer progression and cisplatin resistance through the miR‐4742‐5p/TACC1 axis

Abstract

Recent studies show that lncRNAs participate in drug resistance and nonsmall cell lung cancer (NSCLC) progression. This study aimed to study the roles and mechanisms of long intergenic nonprotein coding RNA 01140 (LINC01140) in regulating NSCLC progression and drug resistance. Real‐time quantitative polymerase chain reaction and western blot analysis were used to detect LINC01140, miR‐4742‐5p, and transforming acidic coiled‐coil 1 (TACC1) expression in NSCLC cells. The interaction between two molecules was examined by luciferase reporter and/or RNA immunoprecipitation assays. Cell invasion, apoptosis, and cisplatin cytotoxicity were assessed by transwell invasion assay, flow cytometry analysis, and CCK‐8 assay, respectively. LINC01140 was downregulated and miR‐4742‐5p was upregulated in NSCLC. LINC01140 inhibited miR‐4742‐5p expression by competitively binding to miR‐4742‐5p, while miR‐4742‐5p targeted TACC1 to inhibit TACC1 expression in NSCLC cells. LINC01140 enrichment repressed the invasive potential and cisplatin resistance and triggered apoptosis, which was reversed by miR‐4742‐5p overexpression. miR‐4742‐5p inhibition suppressed cell invasion and cisplatin resistance and accelerated apoptosis in NSCLC cells, while TACC1 silencing abolished these effects. Mechanistically, LINC01140 positively regulated TACC1 expression by sponging miR‐4742‐5p. In conclusion, LINC01140 inhibited NSCLC progression and cisplatin resistance via functioning as a ceRNA for miR‐4742‐5p to modulate TACC1.

Pleural Mesothelioma: Treatable Traits of a Heterogeneous Disease

“…TACC1 has the ability to transform fibroblasts and, as evidenced by current research, positively regulates the Ras and PI3K signaling pathways to promote transformation and mammary carcinogenesis. The progression of non-small cell lung cancer is halted by the microRNA miR-4742-5p, which inhibits TACC1 expression [123].…”

LINC01140 Targeting miR-452-5p/RGS2 Pathway to Attenuate Breast Cancer Tumorigenesis

“…LINC01140 is a potential regulator of cancer drive or containment in different tumors. LINC01140 inhibits the progression of lung cancer [ 12 ] and sarcomas [ 13 ], whereas it promotes bladder cancer. Li et al have analyzed the cBioPortal database and reported that LINC01140 expression is significantly reduced in the tumor samples from BC patients [ 14 ].…”