Linc02527 promoted autophagy in Intrahepatic cholestasis of pregnancy

Hu, Jianguo; Liu, Li; Gong, Yangyang; Zhang, Lei; Gan, Xiaoliang; Luo, Xiangfeng; Yu, Tinghe; Zhong, Xiaocui; Deng, Xinru; Hu, Lina; Zhang, Zhanyu; Dong, Xin

doi:10.1038/s41419-018-1013-z

Cited by 23 publications

(26 citation statements)

References 50 publications

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“…The circRNA pulldown was performed as previously described 9 . In brief, sodium dodecyl sulfate–polyacrylamide gel electrophoresis, silver staining, and MALDI-TOF-MS were performed sequentially, followed by protein identification.…”

Section: Methodsmentioning

confidence: 99%

CircRAB11FIP1 promoted autophagy flux of ovarian cancer through DSC1 and miR-129

Zhang

Zhu

2021

Cell Death Dis

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At present, no systematic and in-depth study is available on the function and potential mechanisms of circular RNA in autophagy. This study aimed to screen the expression profiles of circRNA, miRNA, and mRNA of ovarian cancer cells induced by Torin 1 (10 µM). The expression profiles of circRNA, miRNA, and mRNA were analyzed with next-generation sequencing technology. CircRAB11FIP1 expression was elevated in epithelial ovarian cancer (EOC) tissues than in normal ovarian tissues. Silencing circRAB11FIP1 inhibited the autophagic flux of ovarian cancer SKOV3 cells. However, circRAB11FIP1 overexpression activated the autophagic flux of ovarian cancer A2780 cells. CircRAB11FIP1-induced autophagy accelerated EOC proliferation and invasion. Also, circRAB11FIP1 directly bound to miR-129 and regulated its targets ATG7 and ATG14. CircRAB11FIP1 bound to desmocollin 1to facilitate its interaction with ATG101. Also, circRAB11FIP1 directly bound to the mRNA of fat mass and obesity-associated protein and promoted its expression. Then, circRAB11FIP1 mediated mRNA expression levels of ATG5 and ATG7 depending on m6A. In general, this study demonstrated that circRAB11FIP1 regulated ATG7 and ATG14 by sponging miR-129. The data suggested that circRAB11FIP1 might serve as a candidate biomarker for EOC diagnosis and treatment.

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Section: Methodsmentioning

confidence: 99%

CircRAB11FIP1 promoted autophagy flux of ovarian cancer through DSC1 and miR-129

Zhang

Zhu

2021

Cell Death Dis

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“…The specific steps of circRNA pulldown are described in our previous study [14]. Briefly, the specific proteins were identified using SDS-PAGE gel electrophoresis, silver stain and MALDI-TOF-MS [14].…”

Section: Circrna Pulldownmentioning

confidence: 99%

“…The primers were purchased from Genepharma. The 2 -ΔΔCT method was used to quantify the gene expression [14].…”

Section: Qpcrmentioning

confidence: 99%

CircMUC16 promotes autophagy of epithelial ovarian cancer via interaction with ATG13 and miR-199a

et al. 2020

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Background: Circular RNA (circRNA) has been proven to play a significant role in multiple types of cancer. However, the expression and role of circRNAs in epithelial ovarian cancer (EOC) remains elusive. Methods: CircRNA and mRNA expression profiles of EOC were screened with sequencing analysis. Gene silencing and over-expression were used to study circRNA function. Cell proliferation and Matrigel invasion assays were used to detect cell proliferation and invasion, respectively. The expression of circRNAs, mRNAs and miRNAs was detected using qPCR. The location of circRNAs was detected using FISH. The expression of proteins was detected using western blot and immunohistochemistry. Results: CircMUC16 had increased expression in EOC tissues as compared to healthy ovarian tissues. The expression of circMUC16 was linked to the progression in stage and grade of EOC. Hence, silencing circMUC16 suppressed autophagy flux of SKOV3 cells. In contrast, ectopic expression of circMUC16 promoted autophagy flux of A2780 cells. CircMUC16-mediated autophagy exacerbated EOC invasion and metastasis. Mechanistically, circMUC16 could directly bind to miR-199a-5p and relieve suppression of target Beclin1 and RUNX1. In turn, RUNX1 elevated the expression of circMUC16 via promotion of its transcription. CircMUC16 could directly bind to ATG13 and promote its expression. Conclusion: This study demonstrated that circMUC16 regulated Beclin1 and RUNX1 by sponging miR-199a-5p. The data suggested that circMUC16 could be a potential target for EOC diagnosis and therapy.

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“…Both genetic and environmental factors participate in the interpretation of the cause of ICP [ 4 ]. Several studies of ICP have revealed altered genes and proteins in many role places, including oxidative stress, cell growth, apoptosis, lipid metabolism, and immune responses [ 5 , 6 ]. Currently, the identification of ICP is verified by symptoms and the serum level of total bile acids (TBA).…”

Section: Introductionmentioning

confidence: 99%

Diagnostic and Prognostic Value of Long Noncoding RNAs as Potential Novel Biomarkers in Intrahepatic Cholestasis of Pregnancy

Zou

Zhao

Wang

et al. 2021

BioMed Research International

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Long noncoding RNAs (lncRNAs) are a class of important regulators participating in various pathological processes. Until now, the role of lncRNAs in the occurrence and development of intrahepatic cholestasis of pregnancy (ICP) has rarely been investigated. The data from microarray screening revealed 58 upregulated and 85 downregulated lncRNAs and 47 upregulated and 71 downregulated mRNAs in ICP patients compared to healthy controls. Bioinformatics analysis revealed biological processes focused on lipid metabolism, apoptosis, cell cycle, cell differentiation, and oxidative stress. Furthermore, the expressions of three lncRNAs (ENST00000505175.1, ASO3480, and ENST00000449605.1) chosen for verification were significantly decreased and showed the diagnostic and prognostic value for ICP based on ROC analysis. This is the first study to report the specific role of lncRNAs in ICP, which may be helpful for the diagnosis and prognosis of ICP clinically.

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Linc02527 promoted autophagy in Intrahepatic cholestasis of pregnancy

Cited by 23 publications

References 50 publications

CircRAB11FIP1 promoted autophagy flux of ovarian cancer through DSC1 and miR-129

CircRAB11FIP1 promoted autophagy flux of ovarian cancer through DSC1 and miR-129

CircMUC16 promotes autophagy of epithelial ovarian cancer via interaction with ATG13 and miR-199a

Diagnostic and Prognostic Value of Long Noncoding RNAs as Potential Novel Biomarkers in Intrahepatic Cholestasis of Pregnancy

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