Lineage-Restricted Neural Precursors Can Be Isolated from Both the Mouse Neural Tube and Cultured ES Cells

Mujtaba, Tahmina; Piper, David R.; Kalyani, Anjali J.; Groves, Andrew K.; Lucero, Mary T.; Rao, Mahendra S.

doi:10.1006/dbio.1999.9418

Cited by 195 publications

(131 citation statements)

References 74 publications

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“…OPCs were immunopanned from embryonic day 16 (E16) Wistar rat spinal cords using an A2B5 antibody and a protocol modified from previous studies (Mayer-Proschel et al 1997;Mujtaba et al 1999;Cao et al 2005). Immunopanned OPCs were plated onto Poly-D-lysine (PDL)/fibronectin-coated 10 × 12 cm culture dishes and growth medium was added and changed every other day.…”

Section: Cell Culturementioning

confidence: 99%

Differential Gene Expression in Oligodendrocyte Progenitor Cells, Oligodendrocytes and Type II Astrocytes

Wang

Zhou

et al. 2011

Tohoku J. Exp. Med.

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Oligodendrocyte precursor cells (OPCs) are bipotential progenitor cells that can differentiate into myelin-forming oligodendrocytes or functionally undetermined type II astrocytes. Transplantation of OPCs is an attractive therapy for demyelinating diseases. However, due to their bipotential differentiation potential, the majority of OPCs differentiate into astrocytes at transplanted sites. It is therefore important to understand the molecular mechanisms that regulate the transition from OPCs to oligodendrocytes or astrocytes. In this study, we isolated OPCs from the spinal cords of rat embryos (16 days old) and induced them to differentiate into oligodendrocytes or type II astrocytes in the absence or presence of 10% fetal bovine serum, respectively. RNAs were extracted from each cell population and hybridized to GeneChip with 28,700 rat genes. Using the criterion of fold change > 4 in the expression level, we identified 83 genes that were up-regulated and 89 genes that were down-regulated in oligodendrocytes, and 92 genes that were up-regulated and 86 that were down-regulated in type II astrocytes compared with OPCs. The up-regulated genes, such as activating transcription factor 3 and myelin basic protein in oligodendrocytes or claudin 11 in type II astrocytes, might contribute to OPC differentiation and represent constitutive components of oligodendrocytes or type II astrocytes. The down-regulated genes in both oligodendrocytes and type II astrocytes, such as transcription factor 19, might be involved in maintaining self-renewal and/or represent the property of OPCs. These results provide new insights into the elucidation of the molecular mechanisms, by which OPCs differentiate to oligodendrocytes or type II astrocytes.

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Section: Cell Culturementioning

confidence: 99%

Differential Gene Expression in Oligodendrocyte Progenitor Cells, Oligodendrocytes and Type II Astrocytes

Wang

Zhou

et al. 2011

Tohoku J. Exp. Med.

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“…OPCs were isolated from the spinal cords of Wistar rat embryos at embryonic day (E) 14 using A2B5 antibody (1:100; Chemicon, Temecula, CA), according to a protocol modified from previous studies (Mayer-Proschel et al 1997;Mujtaba et al 1999;Cao et al 2005;Hu et al 2009). Immunopanned OPCs were plated onto poly-Dlysine (Sigma, St. Louis, MO) and fibronectin (human plasma; BD Biosciences, Bedford, MA)-coated 10 × 12 cm culture dishes, and growth medium was added and changed every other day.…”

Section: Isolation and Culture Of Opcsmentioning

confidence: 99%

BMP Signaling Mediates Astrocyte Differentiation of Oligodendrocyte Progenitor Cells

Lü

Wang

et al. 2010

Tohoku J. Exp. Med.

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Oligodendrocyte precursor cells (OPCs) can differentiate into oligodendrocytes or astrocytes, depending on cellular microenvironments. OPCs, cultured in medium supplemented with 10% (v/v) fetal bovine serum (FBS), give rise to type II astrocytes that express glial fibrillary acidic protein and a cell surface ganglioside that is recognized by A2B5 monoclonal antibody. However, the factors in FBS that direct the astrocyte differentiation are not determined. Moreover, bone morphogenetic proteins (BMPs) have been reported to be involved in astrocyte differentiation of neural progenitor cells. We therefore examined whether BMPs are responsible for the serum-mediated astrocyte differentiation from OPCs. OPCs were isolated from the spinal cords of Wistar rat embryos (at day 14) using the A2B5 antibody. We measured the concentrations of BMP-2 and BMP-4 in FBS and rat and human sera and the expression of mRNAs for three types of BMP receptors (BMPRIa, Ib and II) in OPCs by RT-PCR. The serum samples of the three species contained BMP-2 and BMP-4, as judged by ELISA with each monoclonal antibody, and the BMP receptor mRNAs are expressed in OPCs. When OPCs were cultured in the medium containing 10% FBS, cells (more than 95%) differentiated into type II astrocytes. However, when OPCs were pretreated with noggin, a soluble antagonist of BMP action, the degree of astrocyte differentiation was markedly decreased from 95.39 to 38.36%. Taken together, these results suggest that BMP signaling may be responsible for the serummediated astrocyte differentiation of OPCs. Our findings provide new insights into the molecular basis of differentiation of OPCs.

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“…β-III Tubulin (Menezes and Luskin, 1994) and MAP2 were used as markers of HNPs and neurons (Peng et al, 1986;Kosik and Finch, 1987), and synaptophysin and RT-97 were used as markers of mature neurons (Kahn et al, 1987). A2B5 was used to identify GRPs (Eisenbarth et al, 1979), although it can recognize a subset of NRPs (Mujtaba et al, 1999), GFAP was used to identify astrocytes (Bignami et al, 1972), and O4 and Gal-C were used to identify oligodendrocytes (Bansal et al, 1989). Antibodies against specific neurotransmitters and neurotransmitter-synthesizing enzymes were used to assess specific, mature, neuronal phenotypes: glutamate, glycine, choline acetyltransferase (ChAT), and tyrosine hydroxylase (TH).…”

Section: Immunocytochemistrymentioning

confidence: 99%

Identification and characterization of neuronal precursors and their progeny from human fetal tissue

Piper

Mujtaba

Keyoung

et al. 2001

J of Neuroscience Research

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We have examined primary human neuronal precursors (HNPs) from 18-22-week-old fetuses. We showed that E-NCAM/MAP2/β-III tubulin-immunoreactive neuronal precursors divide in vitro and could be induced to differentiate into mature neurons in 2 weeks. HNPs did not express nestin and differentiated slowly compared to rodent neuronal restricted precursors (NRPs, 5 days). Immunocytochemical and physiological analyses showed that HNPs could generate a heterogeneous population of neurons that expressed neurofilament-associated protein and various neurotransmitters, neurotransmitter synthesizing enzymes, voltage-gated ion channels, and ligand-gated neurotransmitter receptors and could fire action potentials. Undifferentiated and differentiated HNPs did not coexpress glial markers. Only a subset of cells that expressed GFP under the control of the Tα1 tubulin promoter was E-NCAM/β-III tubulin-immunoreactive, indicating nonexclusive overlap between these two HNP cell populations. Overall, HNPs resemble NRPs isolated from rodent tissue and appear to be a neuronal precursor population. Keywords stem cells; differentiation; electrophysiologyMultipotent human neural stem cells (NSCs) have been isolated from fetal human forebrain and spinal cord (Buc-Caron, 1995;Sabate et al., 1995;Svendsen et al., 1996Svendsen et al., , 1998 ChalmersRedman et al., 1997;Johansson et al., 1999; Vescovi et al., 1999a,b;Carpenter et al., 1999;Quinn et al., 1999;Piper et al., 2000) as well as the adult human subependymal zone and hippocampus (Kukekov et al., 1999; Roy et al., 2000a,b). Furthermore, phenotypically restricted lineages have also been reported for human brain tissue. These include glial restricted precursors (GRPs), which have been isolated from adult human brain tissue (Scolding et al., 1999;Roy et al., 1999), and recent reports have suggested the existence of neuronal restricted precursors (NRPs) in both fetal (Raymon et al., 1999;Li et al., 2000) and adult (Roy et al., 2000a,b;Wang et al., 2000) human tissue.NSCs or neuroepithelial precursor cells (NEPs) can generate neurons in vitro (Carpenter et al., 1999;Piper et al., 2000) as well as in vivo (Svendsen et al., 1998;Vescovi et al., 1999b). In vitro, neurons derived from less committed, NEP-like cell populations develop slowly and do ★ Correspondence to: Mary T. Lucero, PhD, Department of Physiology, 410 Chipeta Way, Room 155, Salt Lake City, UT 84108-1297., mary.lucero@m.cc.utah.edu. NIH Public AccessAuthor Manuscript J Neurosci Res. Author manuscript; available in PMC 2010 November 9. NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript not mature fully until after 70 days under differentiating conditions (Chalmers-Redman et al., 1997). Once differentiated, NEP-derived neuronal cells acquired Na + and K + channels but did not develop the ability to fire action potentials despite prolonged culture periods (>3 weeks; Piper et al., 2000). These data suggested that, in human cells, neuronal maturation may require factors other than those described for r...

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Lineage-Restricted Neural Precursors Can Be Isolated from Both the Mouse Neural Tube and Cultured ES Cells

Cited by 195 publications

References 74 publications

Differential Gene Expression in Oligodendrocyte Progenitor Cells, Oligodendrocytes and Type II Astrocytes

Differential Gene Expression in Oligodendrocyte Progenitor Cells, Oligodendrocytes and Type II Astrocytes

BMP Signaling Mediates Astrocyte Differentiation of Oligodendrocyte Progenitor Cells

Identification and characterization of neuronal precursors and their progeny from human fetal tissue

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