Linear and branched pentaamines, hexaamines and heptaamines in seeds of Vicia sativa

Hamana, Koei; Nitsu, Masaru; Samejima, Keijiro; Matsuzaki, Shinya

doi:10.1016/0031-9422(91)83201-u

Cited by 25 publications

(14 citation statements)

References 9 publications

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“…Because the total amount of the two isomers can be determined from the peak intensities of the GC-FID chromatogram of the mixture of the hexaamines (data not shown), the content of each hexamine can be calculated from the ratio and the total amount of the hexaamines. Another linear hexaamine, homohexamine (4-4-4-4-4), found in the seed of a vetch has also been identified using GC resolution and MS [7]. Our current study provides the basis of a new approach for evaluating the amount of long linear polyamine isomers in biological samples.…”

Section: -3-3-4-3 To 3-3-4-3-3 Mixing Ratio or The Ratio Of The M/zmentioning

confidence: 69%

“…Biogenic polyamines are aliphatic molecules that contain more than two amine residues. In addition to the typical polyamines, putrescine, spermidine, and spermine, various kinds of polyamines, including pentaamines and hexaamines, have been found in a variety of organisms ranging from bacteria to plants and animals, such as thermophilic bacteria, thermophilic archaea, leguminous seeds, sea urchins, and spider toxins [1][2][3][4][5][6][7][8][9][10]. These long polyamines are composed of repeating units of three or four methylene spacing units between amine residues.…”

Section: Introductionmentioning

confidence: 99%

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Gas Chromatography-Mass Spectrometry Analysis of Structural Isomers of Linear Hexaamines Found in Thermophilic Bacteria

et al. 2015

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Because of the structural similarity between long linear polyamine isomers, it is often very difficult to differentially quantify each hexaamine found in thermophilic bacteria. To quantify thermohexamine and homothermohexamine, for which separation has not been previously achieved, we analyzed a mixture of the heptafluorobutyryl derivatives of the two authentic hexaamines using gas chromatography-mass spectrometry (GC-MS). We found a correlation between the ratio of the hexaamines and the ratio of the intensities of two unique fragment ions in the MS spectra detected at m/z 774 in thermohexamine and m/z 1027 in homothermohexamine. The ratio of m/z (774/1027) vs. thermohexamine/homothermohexamine and m/z (1027/774) vs. homothermohexamine/thermohexamine calibration lines showed high linearity (r = 0.9997 and 0.9990, respectively) for hexaamine mixing ratios in the range of 0-2.33. These data indicate that the ratio of the hexaamines in a mixed solution can be determined by using the appropriate calibration line, depending on which hexaamine is present in greater quantity.

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Section: -3-3-4-3 To 3-3-4-3-3 Mixing Ratio or The Ratio Of The M/zmentioning

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Gas Chromatography-Mass Spectrometry Analysis of Structural Isomers of Linear Hexaamines Found in Thermophilic Bacteria

et al. 2015

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“…Neither 434 nor 3434 have been detected in any Thermus species (Table 1). Aminobutylcanavalmine (4434) and homopentamine (4444) have been found in some leguminous seeds (Hamana et al, 1991c;Mastuzaki et al, 1990). A natural occurrence of the penta-amine 4334 (tentatively named as bis(aminobutyl)norspermidine, Furuchi et al, 2015b) among the four penta-amine isomers 3434, 3344, 3443 and 4334 remains unknown.…”

Section: T Aquaticus T Antranikianii T Arciformis T Brockianusmentioning

confidence: 98%

Occurrence of two novel linear penta-amines, pyropentamine and homopyropentamine, in extremely thermophilic <i>Thermus composti</i>

Hamana

Furuchi

Hayashi

et al. 2016

J. Gen. Appl. Microbiol.

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None of the authors of this manuscript has any financial or personal relationship with other people or organizations that could inappropriately influence their work. , 2016). Organisms harvested at the early stationary phase were homogenized in 5% perchrolic acid (PCA). The PCA extract was subjected to a column of cation-exchange resin, Dowex 50WX8 (1 cm I.D. ¥ 3 cm), and eluted with 6M HCl to concentrate the polyamines. The concentrated polyamines were analyzed by high-performance (high speed) liquid chromatography (HPLC) on a Hitachi L6000 equipped with a column of cation-exchange resin, Hitachi 2619F (4 mm I.D. ¥ 50 mm) kept at 70∞C. Polyamines were eluted by a linear gradient of NaCl in citrate buffers (0.045 M sodium citrate-0.061 M citric acid-0.063 M NaCl, pH 3.9 and 0.20 M sodium citrate-2.0 M NaCl, pH 7.6) and detected by post-labeled fluorometry using a Hitachi F1050 fluorescence spectrometer after heating with o-phthalaldehyde reagent at 70∞C (Hamana, 2002b RIKEN BioResource Center, Tsukuba, Japan Key Words: homopyropentamine; penta-amine; polyamine; pyropentamine; thermophilic bacteria; Thermus Cellular polyamine distribution profiles in extremophilic bacteria and archaea are related to their optimum growth temperature, pH, and salt concentrations, as well as their phylogenetically classified locations (Hamana, 2002a;Hamana and Hosoya, 2006;Hamana and Matsuzaki, 1992). The distribution of linear penta-amines, linear hexaamines, and branched penta-amines, have been reported in extensive bacterial and archaeal hyper-/extreme thermophiles (Hamana et al

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“…As regards V. sativa most of the research has been focused on its chemical composition with particular attention to the protein content (4-6) and on the presence of antinutritional factors (2,7,8) while no information has been reported on the volatile constituents of this plant. In this paper the leaf volatile fraction of the V. sativa has been studied using HS/SPME-GC/MS; the SPME technique sampling was chosen because it provides simple procedure for solvent-free sample preparation and no sample manipulation is required.…”

Section: Introductionmentioning

confidence: 99%

Headspace Volatiles ofVicia sativaL. (Leguminoseae) by Solid-Phase Microextraction and Gas Chromatography/Mass Spectrometry

Romeo¹,

Verzera²,

Ziino³

et al. 2009

Journal of Essential Oil Research

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The leaf volatile constituents of Vicia sativa L. have been studied using Headspace Solid-Phase Microextraction and Gas Chromatography/Mass Spectrometry (HS/SPME-GC/MS) and GC-FID. Forty-three components, aliphatic hydrocarbons, alcohols, aldehydes and ketones, aromatic aldehydes, esters and alcohols, monoterpenes and sesquiterpenes were fully characterized by mass spectra, linear retention indices, and injection of standards; the average composition as single components and classes of substances is reported. Leaf volatiles in Vicia sativa L. were characterized by a high amount of aldehydes, with the (Z)-2-hexenal (the typical leaf aldehyde) the main component.

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Linear and branched pentaamines, hexaamines and heptaamines in seeds of Vicia sativa

Cited by 25 publications

References 9 publications

Gas Chromatography-Mass Spectrometry Analysis of Structural Isomers of Linear Hexaamines Found in Thermophilic Bacteria

Gas Chromatography-Mass Spectrometry Analysis of Structural Isomers of Linear Hexaamines Found in Thermophilic Bacteria

Occurrence of two novel linear penta-amines, pyropentamine and homopyropentamine, in extremely thermophilic <i>Thermus composti</i>

Headspace Volatiles ofVicia sativaL. (Leguminoseae) by Solid-Phase Microextraction and Gas Chromatography/Mass Spectrometry

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