Linear Mesostructures in DNA–Nanorod Self-Assembly

Vial, Stéphanie; Nykypanchuk, Dmytro; Yager, Kevin G.; Tkachenko, Alexei V.; Gang, Oleg

doi:10.1021/nn401413b

Cited by 96 publications

(100 citation statements)

References 25 publications

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“…[37][38][39][40] And, whereas the current focus mostly was on equilibrated systems, one could also obtain time-dependent properties that depend on the flux of binding groups along the particle surface. These colloids display qualitatively new behavior, including a much broader association/dissociation transition than is common for colloids with immobile binding groups.…”

Section: Resultsmentioning

confidence: 99%

Solid Colloids with Surface-Mobile DNA Linkers

2013

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Surface functionalization with bioinspired binding groups is increasingly used to steer nano- and microscale self-assembly processes, with complementary DNA "sticky ends" as one of the most notable examples. The fabrication of well-organized structures is complicated, however, by the sharp association/dissociation transitions and the slow rearrangement kinetics intrinsic to collections of discrete, surface-immobilized binding groups and is aggravated by natural nonuniformities in the surface coating. Here, we demonstrate a novel system of solid microparticles functionalized with specific binding groups-in this case DNA linkers-that are fully mobile along the particle surface. These colloids display qualitatively new behavior and circumvent many of the commonly encountered issues. Importantly, the association/dissociation transition, and thereby the temperature window for equilibrium self-assembly, is much broader. We further find that the linkers are uniformly distributed above the DNA melting temperature, while visibly accumulating at the interparticle contacts below this temperature. The unique combination of binding group mobility with nondeformability, monodispersity, and facile manipulation of solid particles should have a profound impact on DNA-mediated and other bioinspired self-assembly approaches. Moreover, our highly tunable experimental system enables detailed model investigations that will also deepen our fundamental understanding of other systems with surface-mobile binding groups, for instance, biological ligand-receptor interactions.

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Section: Resultsmentioning

confidence: 99%

Solid Colloids with Surface-Mobile DNA Linkers

2013

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“…One dimensional linear meso-structure of nanorod-DNA assembly has also been looked at by in situ SAXS. 55 The actual solution state mechanism for the formation of citrate-capped gold nanoparticles has also been demonstrated by Kraehnert et al 56 using continuous flow SAXS measurement. 57 There are many other reports on the SAXS study of biological and material interfaces, 54,55,58−62 although until now very few reports existed on noble metal nanoclusters.…”

Section: Introductionmentioning

confidence: 83%

Size Evolution of Protein-Protected Gold Clusters in Solution: A Combined SAXS–MS Investigation

et al. 2015

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ABSTRACT:We report a combined small-angle X-ray scattering (SAXS) and mass spectrometric (MS) study of the growth of gold clusters within proteins, in the solution state. Two different proteins, namely, lysozyme (Lyz) and bovine serum albumin (BSA), were used for this study. SAXS study of clusters grown in Lyz shows the presence of a 0.8 nm gold core, which is in agreement with the Au 10 cluster observed in MS. Dynamic light scattering suggests the size of the cluster core to be 1.2 nm. For BSA, however, a bigger core size was observed, comparable to the Au 33 core obtained in MS. Concentration-and time-dependent data do not show much change in the core size in both SAXS and MS investigations. When metal−protein adducts were incubated for longer time in solution, nanoparticles were formed and protein size decreased, possibly due to the fragmentation of the latter during nanoparticle formation. The data are in agreement with dynamic light scattering studies. This work helps to directly visualize cluster growth within protein templates in solution.

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“…Among this type of NPs, gold NPs have arisen as an option for many biotechnology applications. Drug delivery, biosensing, bioimaging, and photothermal therapy are some of the areas where these particles can be applied . Such versatility is explained by the number of possible surface modifications, optical properties, biocompatibility, and reduced toxicity as compared to other metallic particles .…”

Section: Types Of Nanoparticlesmentioning

confidence: 99%

Nanoparticles for bone tissue engineering

Vieira¹,

Vial²,

Reis³

et al. 2017

Biotechnology Progress

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170

137

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Tissue engineering (TE) envisions the creation of functional substitutes for damaged tissues through integrated solutions, where medical, biological, and engineering principles are combined. Bone regeneration is one of the areas in which designing a model that mimics all tissue properties is still a challenge. The hierarchical structure and high vascularization of bone hampers a TE approach, especially in large bone defects. Nanotechnology can open up a new era for TE, allowing the creation of nanostructures that are comparable in size to those appearing in natural bone. Therefore, nanoengineered systems are now able to more closely mimic the structures observed in naturally occurring systems, and it is also possible to combine several approaches - such as drug delivery and cell labeling - within a single system. This review aims to cover the most recent developments on the use of different nanoparticles for bone TE, with emphasis on their application for scaffolds improvement; drug and gene delivery carriers, and labeling techniques. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:590-611, 2017.

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Linear Mesostructures in DNA–Nanorod Self-Assembly

Cited by 96 publications

References 25 publications

Solid Colloids with Surface-Mobile DNA Linkers

Solid Colloids with Surface-Mobile DNA Linkers

Size Evolution of Protein-Protected Gold Clusters in Solution: A Combined SAXS–MS Investigation

Nanoparticles for bone tissue engineering

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