Linear robust control of S. cerevisiae fed-batch cultures at different scales

Dewasme, Laurent; Richelle, Anne; Dehottay, Philippe; Georges, Patrice; Remy, M.; Bogaerts, Philippe; Wouwer, Alain Vande

doi:10.1016/j.bej.2009.10.001

Cited by 37 publications

(20 citation statements)

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“…In fed-batch processes, highly concentrated fresh medium is fed into the vessel in order to avoid substrate limitation and therefore support growth and production rates, increasing the culture volume over time. The operation lasts longer than in a simple batch process and can lead to high biomass concentrations and/or harvest rates [11]. In continuous mode, "spent" medium can be continuously withdrawn.…”

Section: Introductionmentioning

confidence: 99%

Experimental Validation of a Cascade Control Strategy for Continuously Perfused Animal Cell Cultures

et al. 2020

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This paper is dedicated to the experimental validation of a cascade control strategy for simultaneously regulating the glucose and biomass levels in continuously perfused HEK-293 cell cultures. The inner loop consists of a partial feedback linearization, which requires the estimation of the biomass specific growth rate and glucose uptake rate. This latter task is achieved by sliding mode observers, which do not require a priori process knowledge in the form of a process model. The linearized process is then regulated by the outer loop, including two classical PI controllers with autotuning. The four manipulated variables are a feed flow rate with low glucose concentration, another feed flow with a higher glucose content, a bleed flow, and a perfusion stream. The experimental results demonstrate the ability of the control strategy to reach and regulate the prescribed setpoints. The main advantage of the strategy is that it can be applied in a plug and play manner and shows satisfactory robustness. To the best of our knowledge, this is the first time that such a multivariable control strategy, together with sliding mode observers, is applied at the lab scale to an industrial process in the pharmaceutical sector.

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Section: Introductionmentioning

confidence: 99%

Experimental Validation of a Cascade Control Strategy for Continuously Perfused Animal Cell Cultures

et al. 2020

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“…This phenomenon occurs for instance in S. cerevisiae cultures with aerobic ethanol formation, in P. pastoris with aerobic methanol formation, in E. coli cultures with aerobic acetate formation or in mammalian cell cultures with aerobic lactate formation. To avoid this undesirable effect, a closedloop optimizing strategy is required, which can take various forms (Pomerleau (1990), Chen et al (1995), Renard et al (2006), Dewasme et al (2009)).…”

Section: Introductionmentioning

confidence: 99%

Extended Kalman Filter Design for Acetate Estimation in E. coli Cultures

Dewasme

Goffaux

Hantson

et al. 2011

IFAC Proceedings Volumes

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“…To optimize the culture conditions and to avoid high concentrations of inhibitory by-products, a closed-loop solution is required, and a wide diversity of approaches, e.g., (Pomerleau, 1990;Chen et al, 1995;Rocha, 2003;Renard and Wouwer, 2008;Dewasme et al, 2009a;Dewasme et al, 2009b) have been considered.…”

Section: Introductionmentioning

confidence: 99%

LINEARIZING CONTROL OF YEAST AND BACTERIA FED-BATCH CULTURES - A Comparison of Adaptive and Robust Strategies

Dewasme

Wouwer

Coutinho

2010

Proceedings of the 7th International Conference on Informatics in Control, Automation and Robotics

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Linearizing control is a popular approach to control bioprocesses, which has received considerable attention is the past several years. This control approach is however quite sensitive to modeling uncertainties, thus requiring some on-line parametric adaptation so as to ensure performance. In this study, this usual adaptive strategy is compared in terms of implementation and performance to a robust strategy, where the controller has a fixed parametrization which is determined using a LMI framework so as to ensure robust stability and performance. Fed-batch cultures of yeast and bacteria are considered as application examples.

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Linear robust control of S. cerevisiae fed-batch cultures at different scales

Cited by 37 publications

References 10 publications

Experimental Validation of a Cascade Control Strategy for Continuously Perfused Animal Cell Cultures

Experimental Validation of a Cascade Control Strategy for Continuously Perfused Animal Cell Cultures

Extended Kalman Filter Design for Acetate Estimation in E. coli Cultures

LINEARIZING CONTROL OF YEAST AND BACTERIA FED-BATCH CULTURES - A Comparison of Adaptive and Robust Strategies

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