Linkage betwen the male sterility gene (ms₁) and a translocation breakpoint in soybean, Glycine max

Abstract

SACKS, J. M., and K. SADANAGA. 1984. Linkage between the male sterility gene ( m s , ) and a translocation breakpoint in soybean, Glycine max. Can. J. Genet. Cytol. 26: 40 1 -404. The F2 distribution of male sterility ( m s and flower color ( M,,) in a cross between translocation 172-1 1-3 ( W, Wl Ms , Ms ,) and A77-139 (u, w , Ms ms , ) in soybean, Glycine ma,u (L.) Merr., was highly abnormal. Duplicate-deficient and 41-chromosome progenies constituted 32% of the Fz population. The M , , and ms I loci are located on the translocation exchange chromosomes designated TI and T2, respectively. Evidence from other translocations suggests that linkage group 8 (LG8) is located on the single satellited normal chromosome designated N I . The breakpoint lies between the ms , and w , loci with recombination values of 18.4 2 3.3% and 2.0 -+ I .2%, respectively. Key ~9ord.s: I inkage. translocation. soybean, Glycine. SACKS, J. M.. et K. SADANAGA. 1984. Linkage between the male sterility gene ( m s , ) and a translocation breakpoint in soybean, Glycine m m . Can. J. Genet. Cytol. 26: 401 -404. La distribution en F, de la stirilitk mile (m.7,) et de la couleur des fleurs (M,,) dans un croisement entre les translocations 172-1 1-3 ( W I W , M s I Ms I ) et A77-139 ( M, I M,, Ms ms I ) chez le soya, Glyc-ine mau (L. ) Merr., s'est avkrke hautement abnormale. Une dkficience de duplication et des descendants a 41 chromosomes ont constituk 32% de la population F2. Les loci w , et m s , sont localisks sur les chromosomes d'kchange de translocation design& T I et TI, respectivement. Une kvidence provenant d'autres translocations suggere que le groupe de linkage 8 (LG8) est localisk sur un chromosome normal a un seul satellite dksignk Nl. Le point de rupture se situe entre les loci m s , et \rl, avec des valeurs de recombinaison de 18,4 + 3,3 et 2,O -+ 1,29". respectivement.

Translocation Breakpoints in Soybean Classical Genetic Linkage Groups 6 and 8

“…Using standard linkage estimation methods, Palmer (1985) reported linkage between the breakpoint in Clark T/T and Y 11 (18.4 ± 0.4) and Df 2 (5.8 ± 0.2), and also between the breakpoint and Ms 1 (8.5 ± 1.5), Wm (36.8 ± 1.6), and W 1 (39.5 ± 2.0), thus placing the breakpoint between Ms 1 and Df 2 The values reported in our study for CLG 8 are in agreement with those reported by Palmer and Kaul (1983) and Palmer (1985), but different from Sadanaga and Grindeland (1984) and Sacks and Sadanaga (1984) These differences may be reflective of the different methods (F 2 and F 2:3 segregating data vs. microscope observations) used to obtain linkage data and the different generations of self‐pollination that occurred before the translocations were used in the different studies. Whereas Sadanaga and Grindeland (1984) and Sacks and Sadanaga (1984) used the translocations shortly after their isolation following mutagenesis, five generations of self‐pollination occurred before they were used in our study. Hence, their recombination values would have been subject more to any unknown instabilities or biases due to differential gametic or genotypic viabilities.…”

“…On the basis of the observation of chromosome number and constitution during meiosis, Sacks and Sadanaga (1984) reported linkage between W 1 and the breakpoint (2.0 ± 1.2) and Ms 1 and the breakpoint (18.4 ± 3.3), while Sadanaga and Grindeland (1984) reported linkage between the breakpoint and W 1 (1.9 ± 0.8) in KS172‐11‐3, and independent assortment with T and Y 10 Both studies placed the breakpoint distal to W 1 , and Sacks and Sadanaga (1984) placed the breakpoint between W 1 and Ms 1…”

“…Earlier studies (Palmer and Kilen, 1987) have resulted in the isolation of six reciprocal translocations (Table 1) Cytogenetic studies have shown that the translocations KS172‐11‐3, KS175‐7‐3, and Clark T/T share a common chromosome that is different from the one shared by KS171‐31‐2, PI 189866, and L75‐0283‐4 (Sadanaga and Newhouse, 1982; Sellner, 1990; Mahama et al, 1999). Sadanaga and Grindeland (1984) and Sacks and Sadanaga (1984) used the KS172‐11‐3 translocation in linkage studies and reported a gene order of W 1 , Wm , breakpoint, Ms 1 Using the Clark T/T translocation, Palmer (1985), and Palmer and Kaul (1983) reported a gene order of W 1 , Wm , Ms 1 , breakpoint. In a separate study with CLG 6 loci, Palmer (1985) reported a gene order of Y 11 , Df 2 , breakpoint.…”

Cytogenetics of a reciprocal translocation integrating distichous pedicel and tendril-less leaf mutations inLathyrus sativusL.

“…An individual heterozygous for a reciprocal translocation exhibits partial pollen and ovule sterility (40-60%), and this character defines the translocation breakpoint showing linkage with mutations of the two different linkage groups (Mahama and Palmer 2003). Associations between genetic markers and the translocation breakpoints were studied in soybean (Palmer and Kaul 1983;Sacks and Sadanaga 1984;Mahama et al 2002), lentil (Tadmor et al 1987) and pea (Berdnikov et al 1994). In grass pea, multiple interchanges have been detected among different chromosomes (Talukdar 2010), and a linked relationship between a flower colour mutation and translocation breakpoint has recently been elucidated (Talukdar 2011a).…”