Linkage Mapping in Rhizobium leguminosarum by means of R Plasmid-mediated Recombination

Beringer, J. E.; Hoggan, Shelagh A.; Johnston, Andrew

doi:10.1099/00221287-104-2-201

Cited by 115 publications

(65 citation statements)

References 21 publications

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“…Plasmids may also associate with a number of chromosomal sites resulting in the simultaneous transport of many regions of the chromosome (Coetzee, 1978 b ;Haas & Holloway, 1976;Beringer et al, 1978). The result is that the polarized gene transfer of any one mobilizing event is scrambled in the population as a whole (Meade & Signer, 1977).…”

Section: Discussionmentioning

confidence: 99%

“…Similar findings were recorded with plasmid R772 (Coetzee, 1978b) and this is what would be expected if R394 had many sites of association with the chromosome. All progeny were stable through many purification cycles on non-selective media and behaved like haploid recombinants (Meade & Signer, 1977;Beringer et al, 1978).…”

Section: R E S U L T Smentioning

confidence: 96%

“…Single R plasmids of the multiple chromosomal association type, on the other hand, have been used in plate-mating experiments to derive linkage data which indirectly established circularity of chromosome linkage groups in Rhizobium meliloti (Meade & Signer, 1977;Kondorosi et al, 1977) and Rhizobium Ieguminosarum (Beringer et a]., 1978).…”

Section: Discussionmentioning

confidence: 99%

“…Results of actual crosses are not presented. Although co-inheritance data do not necessarily relate to physical distance between markers (see Beringer et al, 1978), they were used as a measure of linkage between pairs of markers to place the latter in a most probable order (Fig. 1).…”

Section: T----fmentioning

confidence: 99%

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Genetic Circularity of the Proteus mirabilis Linkage Map

Coetzee

1979

Journal of General Microbiology

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171The T incompatibility group plasmid R394 can mobilize the chromosome of Pruteus mirabilis strain ~~5 0 0 6 .It transferred relatively large segments, corresponding to at least 20 inin on the D plasmid chromosomal map of the organism. The frequency of recombination for a large number of selected markers was nearly constant at 5 x lo-* per donor cell and it is concluded that mobilization takes place from a number of chromosomal sites. All recombinants were R f and displayed all properties of the plasmid. By analysing crosses for co-inheritance frequencies of unselected markers, a number of chromosomal loci were assembled in linear array. Linkage between markers at the ends of this linkage group was established to markers at the respective termini of the existing D plasmid linkage group. This established a composite circular linkage map of genes of the P. mirabilis strain ~~5 0 0 6 chromosome. I N T R O D U C T I O NMobilization of the chromosome of P. mirabilis strain ~~5 0 0 6 by the recombinant plasmid P-lacRldrd19 has been described (Coetzee, 1975). Polarized transfer occurred from an origin near his-2 and involved a gradient of eight markers. As a result of a modification in the conjugation procedure (Coetzee, 1978a) the linkage map was extended by a further eight chromosomal markers. This plasmid is now named plasmid D (Coetzee, 1978a).R plasmid R772 can also mobilize the ~~5 0 0 6 chromosome (Coetzee, 1978b). R772-mediated transfer occurred from a number of chromosomal sites and, possibly because predominantly short segments of chromosome were mobilized, co-inheritance of the terminal markers of the D plasmid-derived linkage group could not be demonstrated.A number of other R plasmids may mobilize the ~~5 0 0 6 chromosome (J. N. Coetzee and R. W. Hedges, unpublished observations) and the use of one of these, R394 (Coetzee et d., 1972), to establish indirectly the genetic circularity of the ~~5 0 0 6 chromosomal linkage group is described here. M E T H O D SBacteria and plasmid. These are listed in Table 1. Media. These were as described previously (Coetzee, 1978 b). Antibacterial drugs. Nalidixic acid, ampicillin, kanamycin, tetracycline (each 50 pg ml-I) or rifampicin (200 ,ug ml-I) were added to media when necessary.Plate matings. These were done by the method of Coetzee (1978a). Briefly, mating mixtures were constituted on non-selective MM (i.e. supplemented with growth requirements of recipient and donor) and incubated overnight before harvesting in saline. Control experiments lacked either donor or recipient. After washing, suitable dilutions were plated on selective MM and incubated for 48 h.Consfruction of P. mirabilis UP strains containing plasmid R394. The liquid mating technique of Coetzee et al. (1973) was used. Donor ~53(R394) was counterselected with tetracycline and transfer of the plasmid was selected with kanamycin or ampicillin. Transfer of the markers of plasmid R394 to various UP strains occurred at frequencies of about 1 x per donor cell. All donor strains were maintained on nutrient ...

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Section: Discussionmentioning

confidence: 99%

Section: R E S U L T Smentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: T----fmentioning

confidence: 99%

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Genetic Circularity of the Proteus mirabilis Linkage Map

Coetzee

1979

Journal of General Microbiology

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“…Plasmid R772 is the latest addition to the P-1 incompatibility group plasmids (Bryan et a/., 1973;Haas & Holloway, 1976) which can promote chromosome transfer in particular bacterial species. Chromosomal transfer by P plasmids may be strain specific (Stanisich & Holloway, 1971 ;Stanisich & Richmond, 1975).…”

Section: Discussionmentioning

confidence: 99%

Mobilization of the Proteus mirabilis Chromosome by R Plasmid R772

Coetzee

1978

Journal of General Microbiology

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The P-1 incompatibility group plasmid R772 can mobilize the chromosome of Proteus mirabilis strain ~~5 0 0 6 .The decreasing gradient of recombinant recovery frequencies found for markers which were increasingly distal to 0 min with plasmid D donors was not found with R772. Instead, it produced recombinants for all markers at frequencies of about 5 x per donor. This is about 10-fold lower than the plasmid transfer frequency. Recombinants were stable and recombination was only detected over short segments of the chromosome which corresponded to about 10 min on the D plasmid map of the chromosome. All recombinants had inherited R772 and expressed all properties of the plasmid. Attempts to isolate variant plasmids with increased frequencies of recombinant formation were unsuccessful.

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Taxonomy and Metabolism of Rhizobium and its Genetic Relationships

Elkan

1984

Biological Nitrogen Fixation

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Linkage Mapping in Rhizobium leguminosarum by means of R Plasmid-mediated Recombination

Cited by 115 publications

References 21 publications

Genetic Circularity of the Proteus mirabilis Linkage Map

Genetic Circularity of the Proteus mirabilis Linkage Map

Mobilization of the Proteus mirabilis Chromosome by R Plasmid R772

Taxonomy and Metabolism of Rhizobium and its Genetic Relationships

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