Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos

Silva, Carla O.; Novais, Sara C.; Alves, Luís M.F.; Soares, Amadeu M.V.M.; Barata, Carlos; Lemos, Marco F.L.

doi:10.1016/j.cbpc.2019.108570

Cited by 16 publications

(10 citation statements)

References 37 publications

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“…Regarding the neuronal parameter, AChE is involved in the regulation of the transmission of nerve impulses, and contaminants such as chlorpyrifos has been demonstrated to inhibit AChE activity in G. umbilicalis [17]. However, in this work, a significant induction of AChE was observed in G. umbilicalis exposed to 0.14 and 0.47% exudate concentrations.…”

Section: Discussioncontrasting

confidence: 56%

Asparagopsis armata Exudate Cocktail: The Quest for the Mechanisms of Toxic Action of an Invasive Seaweed on Marine Invertebrates

Silva¹,

Simões²,

Félix³

et al. 2020

Preprint

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The red seaweed Asparagopsis armata exhibits a strong invasive behaviour and is included in the list of the “Worst invasive alien species threatening biodiversity in Europe”. This seaweed has been shown to produce a large diversity of halogenated compounds with effective biological effects, deeply affecting rockpool species. Therefore, the present study aimed to investigate the biochemical responses to sublethal concentrations of Asparagopsis armata exudate on two coastal organisms, the marine snail Gibbula umbilicalis and the rockpool shrimp Palaemon elegans. Antioxidant defences superoxide dismutase (SOD) and glutathione-S-transferase (GST), oxidative damage endpoints lipid peroxidation (LPO) and DNA damage, the neuronal parameter acetylcholinesterase (AChE), as well as the fatty acid profile were evaluated. Results revealed different metabolic responses between species, indicating that A. armata exudate affected the organisms through different pathways. Despite previous studies indicating that the exudate effected G. umbilicalis’ survival and behaviour, this does not seem to result from oxidative stress or addressed neurotoxicity. On the other hand, for P. elegans, an inhibition of AChE and the decrease of antioxidant capacity concomitant with the increase of LPO, suggests neurotoxicity and oxidative stress as mechanisms of exudate toxicity for this species. For fatty acids, there were different profile changes between species, also more pronounced for P. elegans with a general increase in PUFA with exudate exposure, which commonly means a defence mechanism protecting from membrane disruption. Nonetheless, the omega-3 PUFAs ARA and DPA were increased in both invertebrates, indicating a common mechanism regulation of inflammation and immunity responses to this stress. This work provides further insight on the mechanisms of invertebrate response and tolerance to an expanding coastal environmental stress as is the marine invader A. armata.

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Section: Discussioncontrasting

confidence: 56%

Asparagopsis armata Exudate Cocktail: The Quest for the Mechanisms of Toxic Action of an Invasive Seaweed on Marine Invertebrates

Silva¹,

Simões²,

Félix³

et al. 2020

Preprint

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“…Feeding activity have been shown to be sensitive tools to assess the impact of contaminants at concentrations far below lethal levels, which often is related to a decrease of fitness and capacity to respond to contaminants and also often reflects alterations in movement capability, and that will reduce energetic input and metabolism (Cabecinhas et al, 2015; C. O. Silva et al, 2019;Dell'Omo, 2002;Fong & Molnar, 2013). Enzymes involved in energy production have been frequently used as biomarkers to assess the effects of stressors, since exposed organisms usually need additional energy to maintain physiological/biochemical functions (Aderemi et al, 2018;Kühnhold et al, 2017;Silva et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Impacts of The Invasive Seaweed Asparagopsis armata Exudate on Rockpool Invertebrates

Co¹,

Sc²,

Soares³

et al. 2020

Preprint

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The marine red algae Asparagopsis armata is an invasive species which competitive advantage arises from the production and release of large amounts of toxic compounds to the surrounding invaded area, reducing the abundance of native species. The main objective of this study was to evaluate the effects of this invasive seaweed on marine invertebrates by exposing the common prawn Palaemon elegans and the marine snail Gibbula umbilicalis to the exudate of this macroalgae. The seaweed was collected and placed in tanks, for 12 hours, in the dark in a 1:10 ratio. Afterwards the media containing its secondary metabolites was collected for further testing. Lethal and sublethal effects of A. armata were investigated. Biochemical biomarkers responses associated with energy metabolism (lactate dehydrogenase, LDH; electron transport system activity, ETS; content in lipids, proteins and carbohydrates) were analysed. The biomarker responses showed invertebrates’ physiological status impairment after exposure to low concentrations of this algae exudate. Highest concentrations of exudate significantly increased lipid content in both organisms. In the shrimp, protein content, ETS, and LDH were also significantly increased. On the contrary, these parameters were significantly decreased in G. umbilicalis. A behavioural impairment was also observed in G. umbilicalis exposed to A. armata exudate, with reduction in feeding consumption. These results represent an important step in the research of natural toxic exudates released to the environment and prospective effects of this seaweed in invaded communities under increasing global change scenarios.

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“…To perform the biomarker analysis, the whole soft tissue was used for G. umbilicalis [17,19,23], while for P. elegans, since this organism is easier to dissect for specific organs with reported activities, the hepatopancreas was used for oxidative stress and fatty acid endpoints [24], and the eyes were used for neuromuscular endpoints [25].…”

Section: Biochemical Analysis 231 Tissue Preparationmentioning

confidence: 99%

“…The AChE activity was determined according to the methodology proposed by Ellman et al [35] adapted to microplate for these species, as described in more detail in [17,25]. The absorbance was read at 414 nm for 5 min, monitoring the formation of 5-thio-2nitrobenzoate anion (TNB), which results from the reaction of 5,5 -dithiobis-(2-nitrobenzoic acid) (DTNB) with thiocholine, a product of the acetylcholine substrate hydrolysis performed by AChE.…”

Section: Neuromuscular Biomarkermentioning

confidence: 99%

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Asparagopsis armata Exudate Cocktail: The Quest for the Mechanisms of Toxic Action of an Invasive Seaweed on Marine Invertebrates

Silva

Simões

Félix

et al. 2021

Biology

Self Cite

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The seaweed Asparagopsis armata exhibits a strong invasive behavior, producing halogenated compounds with effective biological effects. This study addresses the biochemical responses to sublethal concentrations of A. armata exudate on the marine snail Gibbula umbilicalis whole body and the shrimp Palaemon elegans eyes and hepatopancreas. Antioxidant defenses superoxide dismutase (SOD) and glutathione-S-transferase (GST), oxidative damage endpoints lipid peroxidation (LPO) and DNA damage, the neuronal parameter acetylcholinesterase (AChE), and the fatty acid profile were evaluated. Results revealed different metabolic responses in both species. Despite previous studies indicating that the exudate affected G. umbilicalis’ survival and behavior, this does not seem to result from oxidative stress or neurotoxicity. For P. elegans, the inhibition of AChE and the decrease of antioxidant capacity is concomitant with the increase of LPO, suggesting neurotoxicity and oxidative stress as contributor mechanisms of toxicity for this species. Fatty acid profile changes were more pronounced for P. elegans with a general increase in polyunsaturated fatty acids (PUFAs) with the exudate exposure, which commonly means a defense mechanism protecting from membrane disruption. Nonetheless, the omega-3 PUFAs arachidonic acid (ARA) and docosapentaenoic acid (DPA) increased in both invertebrates, indicating a common regulation mechanism of inflammation and immunity responses.

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Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos

Cited by 16 publications

References 37 publications

<em>Asparagopsis armata</em> Exudate Cocktail: The Quest for the Mechanisms of Toxic Action of an Invasive Seaweed on Marine Invertebrates

<em>Asparagopsis armata</em> Exudate Cocktail: The Quest for the Mechanisms of Toxic Action of an Invasive Seaweed on Marine Invertebrates

Impacts of The Invasive Seaweed <em>Asparagopsis armata</em> Exudate on Rockpool Invertebrates

Asparagopsis armata Exudate Cocktail: The Quest for the Mechanisms of Toxic Action of an Invasive Seaweed on Marine Invertebrates

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