Linking peptide-oriented surface imprinting magnetic nanoparticle with carbon nanotube-based fluorescence signal output device for ultrasensitive detection of glycoprotein

Yu, Shi-Song; Shi, Yujun; Wang, Di; Qiang, Titi; Zhao, Yaqi; Wang, Xinyu; Zhao, Jia-Meng; Dong, Lijie; Huang, Y.; Wang, Xian-Hua

doi:10.1016/j.aca.2023.341202

Cited by 4 publications

(3 citation statements)

References 40 publications

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“…Notably, the biosensor still had a good linear response to HER2 even in a 100% FBS solution. As illustrated in Table S1, compared with the previously reported methods for HER2 detection, the linear range and LOD of the biosensor have obvious advantages. − ,,− …”

Section: Resultsmentioning

confidence: 99%

“…Rapid and reliable identification of human epidermal growth factor receptor 2 (HER2) levels in complex biological samples, including blood and serum, stands as a pivotal requirement for the early diagnosis of cancers . Currently, numerous analytical techniques have been employed to detect HER2, such as fluorescent, optical, and electrochemical assays. − Electrochemical detection is widely employed for biomolecule detection in biological samples, capitalizing on its rapid detection and high sensitivity. , However, the exposure of sensing devices to biological fluids results in biofouling due to the nonspecific adsorption of biological macromolecules, particularly proteins, thereby affecting the accuracy of detection and causing device malfunctions. − To significantly enhance the detection accuracy in electrochemical biosensors, one effective approach is the modification of antifouling materials at the sensing interface. These materials include poly(ethylene glycol) (PEG), zwitterionic materials like sulfobetaine and phosphorylcholine, hyaluronic acid, peptides, − and so on.…”

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confidence: 99%

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Phospholipid Bilayer Integrated with Multifunctional Peptide for Ultralow-Fouling Electrochemical Detection of HER2 in Human Serum

Li,

Han,

Feng

et al. 2023

Anal. Chem.

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Electrochemical biosensing devices face challenges of severe nonspecific adsorption in complex biological matrices for the detection of biomarkers, and thus, there is a significant need for sensitive and antifouling biosensors. Herein, a sensitive electrochemical biosensor with antifouling and antiprotease hydrolysis ability was constructed for the detection of human epidermal growth factor receptor 2 (HER2) by integrating multifunctional branched peptides with distearoylphosphatidylethanolamine-poly(ethylene glycol) (DSPE-PEG) self-assembled bilayer. The peptide was designed to possess antifouling, antiprotease hydrolysis, and HER2 recognizing capabilities. Molecular dynamics simulations demonstrated that the DSPE was able to effectively self-assemble into a bilayer, and the water contact angle and electrochemical experiments verified that the combination of peptide with the DSPE-PEG bilayer was conducive to enhancing the hydrophilicity and antifouling performance of the modified surface. The constructed HER2 biosensor exhibited excellent antifouling and antiprotease hydrolysis capabilities, and it possessed a linear range of 1.0 pg mL–1 to 1.0 μg mL–1, and a limit of detection of 0.24 pg mL–1. In addition, the biosensor was able to detect HER2 in real human serum samples without significant biofouling, and the assaying results were highly consistent with those measured by the enzyme-linked immunosorbent assay (ELISA), indicating the promising potential of the antifouling biosensor for clinical diagnosis.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Phospholipid Bilayer Integrated with Multifunctional Peptide for Ultralow-Fouling Electrochemical Detection of HER2 in Human Serum

Li,

Han,

Feng

et al. 2023

Anal. Chem.

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“…Nanomaterials with a large surface area can be used as carriers to load fluorescent dyes as signal labels for sandwich assays. Yu et al reported a MIP-coated MNP-based biosensor for the detection of glycoproteins using boronic acid-modified carbon nanotubes to load FITC molecules [ 184 ]. Bai et al developed a fluorescent sandwich biosensor for the detection of HRP and CEA [ 185 ].…”

Section: Boronate-affinity-based Fluorescent Assays and Imagingmentioning

confidence: 99%

Biosensors with Boronic Acid-Based Materials as the Recognition Elements and Signal Labels

Liu

Chang

et al. 2023

Biosensors

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It is of great importance to have sensitive and accurate detection of cis-diol-containing biologically related substances because of their important functions in the research fields of metabolomics, glycomics, and proteomics. Boronic acids can specifically and reversibly interact with 1,2- or 1,3-diols to form five or six cyclic esters. Based on this unique property, boronic acid-based materials have been used as synthetic receptors for the specific recognition and detection of cis-diol-containing species. This review critically summarizes the recent advances with boronic acid-based materials as recognition elements and signal labels for the detection of cis-diol-containing biological species, including ribonucleic acids, glycans, glycoproteins, bacteria, exosomes, and tumor cells. We also address the challenges and future perspectives for developing versatile boronic acid-based materials with various promising applications.

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An intelligent solvent-responsive surface molecularly imprinted membrane with switchable adsorption/desorption performance for selective separation of psoralen and recognition mechanism

Luo,

Xu,

Tian

et al. 2024

Industrial Crops and Products

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Linking peptide-oriented surface imprinting magnetic nanoparticle with carbon nanotube-based fluorescence signal output device for ultrasensitive detection of glycoprotein

Cited by 4 publications

References 40 publications

Phospholipid Bilayer Integrated with Multifunctional Peptide for Ultralow-Fouling Electrochemical Detection of HER2 in Human Serum

Phospholipid Bilayer Integrated with Multifunctional Peptide for Ultralow-Fouling Electrochemical Detection of HER2 in Human Serum

Biosensors with Boronic Acid-Based Materials as the Recognition Elements and Signal Labels

An intelligent solvent-responsive surface molecularly imprinted membrane with switchable adsorption/desorption performance for selective separation of psoralen and recognition mechanism

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